SP-D(人)ELISA试剂盒                                             货号：E-HK335

SP-D, Human, ELISA kit

背景资料：The human SP-D ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3·5 hours· The efficient format of 2 plates with twelve disposable 8-well strips allows free choice of batch size for the assay· Samples and standards are incubated in microtiter wells coated with antibodies recognizing human SP-D· Biotinylated tracer antibody will bind to captured human SP-D· Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody· Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB)· The enzyme reaction is stopped by the addition of oxalic acid· The absorbance at 450 nm is measured with a spectrophotometer· A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human SP-D standards (log)· The human SP-D concentration of samples, which are run concurrently with the standards, can be determined from the standard curve··

产品描述：Surfactant protein D (SP-D) belongs to the collectin familiy· These proteins are oligomeric proteins composed of carbohydrate-recognition domains (CRD) attached to collagenous regions· Collectins are structurally similar to the ficolins although they make use of different CRD structures: C-type lectin domain for the collectins· The lung is the major site of synthesis of SP-D, where the molecules are produced and secreted onto the epithelial surface by alveolar type II cells and unciliated bronchial epithelial cells· SP-D is also found in different epithelial cells of the gastrointestinal tract and in epithelial cells of various exocrine glands· SP-D is an important factor in the pulmonary anti-microbial defense· The anti-microbial defense mechanisms of SP-D are direct opsonization, neutralization and agglutination· This results in limiting the infection and concurrently orchestrating the subsequent adaptive immune response· SP-D synthesis and secretion increase significantly during inflammatory stress· In inflamed lungs increased amounts of SP-D in bronchial lavage fluid and tissue are found, particularly in type II pneumocytes, Clara cells and hyperplastic goblet cells are found in inflamed lungs· The localization of SP-D in endocytic vesicles and in lysosomal granules of alveolar macrophages suggests a receptor-mediated uptake· SP-D is also involved the apoptotic process· SP-D binds to apoptotic neutrophils and enhances their clearance by alveolar macrophages· Although complete deficiency of SP-D has not been described in man, basal levels are highly variable and are largely genetically determined· SP-D levels in healthy individuals vary from 200 ng/ml to 5200 ng/ml· Mean SP-D levels increase from 700 ng/ml in children to 1100 ng/ml in adults· Bronchoalveolar fluid SP-D control levels range from 7 ng/ml to 28 um/ml· SP-D levels can rise dramatically during acute pulmonary infections and are raised in certain non-infectious lung diseases· The ELISA is not influenced by the presence of lipopolysaccharides··

产品特点：Detection level 6·3 ng/ml

保存建议：推荐4℃保存，有效期一年。

定购信息： 

产品名称	规格	操作手册	货号	询价
BPI(人)ELISA试剂盒	1 x 96 det	PDF	E-HK335-01
BPI(人)ELISA试剂盒	2 x 96 det	PDF	E-HK335-02
VisionBlue快速细胞活力分析试剂盒	500 次	PDF	C-K303-500

表观遗传学产品全面解决方案列下：

产品名称	规格	操作手册	货号	询价
DNA羟甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1037-48
DNA羟甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1037-96
DNA羟甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1036-48
DNA羟甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1036-96
DNA甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1035-48
DNA甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1035-96
DNA甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1034-48
DNA甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1034-96
通用DNA甲基化试剂盒(定性分析)	48 次	PDF	A-P-1011-48
通用DNA甲基化试剂盒(定性分析)	96 次	PDF	A-P-1011-96
超敏DNA甲基化定量试剂盒	48 次	PDF	A-P-1021-48
超敏DNA甲基化定量试剂盒	96 次	PDF	A-P-1021-96
总DNA甲基化定量试剂盒	48 次	PDF	A-P-1014-48
总DNA甲基化定量试剂盒	96 次	PDF	A-P-1014-96

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