H-无花果酶(人)ELISA试剂盒                                            货号：E-HK340

H-ficolin, Human, ELISA kit

背景资料：The human H-ficolin ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3·5 hours· The efficient format of 2 plates with twelve disposable 8-well strips allows free choice of batch size for the assay· Samples and standards are captured by a solid bound specific antibody· Biotinylated tracer antibody will bind to captured H-ficolin· Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody· Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB)· The enzyme reaction is stopped by the addition of citric acid· The absorbance at 450 nm is measured with a spectrophotometer· A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the H-Ficolin standards (log)· The human H-ficolin concentration of samples, which are run concurrently with the standards, can be determined from the standard curve·.

产品描述：Ficolins are a group of proteins containing both a collagen-like domain and a fibrinogen-like domain· Three forms of Ficolin have been identified in humans: L-ficolin, M-ficolin and H-ficolin· H-ficolin, also known as Hakata-antigen or Ficolin-3, is composed by a collagen-like strand and three C-terminal recognition domains which bind to acetyl groups on microbial surfaces such as GlcNAc or GalNAc· H-Ficolin is synthesized both in the liver, from where it is secreted into the blood circulation, and in the lung· Similar to MBL and L-ficolin, H-ficolin relies on MBL-associated serine protease 2 (MASP-2) for activation of the complement system· After binding of H-ficolin/MASP-2 complexes to microbial surfaces, MASP-2 sequentially cleaves C4 and C2, thereby generating the C3 convertase C4bC2b, which finally leads to opsonization and direct lysis of pathogens and recruitment of inflammatory cells· H-ficolin is present in serum at mean concentration of 15 ug/ml, with only minor variations· H-ficolin was present in all sera from more than 150,000 individuals tested, except in some systemic lupus erythromatosis patients· Approximately 5% of systemic lupus erythromatosis patients were found to be H-ficolin negative, probably owing to the presence of anti-H-ficolin autoantibody· In 398 patients with other autoimmune diseases, H-ficolin was always present· In liver disease the serum levels decreased with increasing severity of cirrhosis· H-ficolin bound to a population of late apoptotic cells, while a strong and uniform binding to necrotic cells was observed· The binding properties differed from those of MBL and L-ficolin· H-ficolin binding to late apoptotic cells resulted in a significant increase in adhesion/uptake by macrophages··

产品特点：The human H-ficolin ELISA has been developed for the quantitative measurement of natural H-ficolin in plasma, and serum· In plasma samples, H-ficolin can be measured accurately if samples are diluted at least 150 times·

保存建议：推荐4℃保存，有效期一年。

定购信息： 

产品名称	规格	操作手册	货号	询价
H-无花果酶(人)ELISA试剂盒	1 x 96 det	PDF	E-HK340-01
H-无花果酶(人)ELISA试剂盒	2 x 96 det	PDF	E-HK340-02
VisionBlue快速细胞活力分析试剂盒	500 次	PDF	C-K303-500

表观遗传学产品全面解决方案列下：

产品名称	规格	操作手册	货号	询价
DNA羟甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1037-48
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DNA羟甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1036-48
DNA羟甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1036-96
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DNA甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1034-96
通用DNA甲基化试剂盒(定性分析)	48 次	PDF	A-P-1011-48
通用DNA甲基化试剂盒(定性分析)	96 次	PDF	A-P-1011-96
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超敏DNA甲基化定量试剂盒	96 次	PDF	A-P-1021-96
总DNA甲基化定量试剂盒	48 次	PDF	A-P-1014-48
总DNA甲基化定量试剂盒	96 次	PDF	A-P-1014-96

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