穿透素3(人)ELISA试剂盒                                            货号：E-HK347

Pentraxin 3, Human, ELISA kit

背景资料：he human pentraxin 3 ELISA has been developed for the quantitative measurement of natural and recombinant human pentraxin 3 in plasma and cell culture medium· In plasma samples human pentraxin 3 can be measured accurately if serum or plasma samples are diluted at least 4 times·

产品描述：Pentraxins are a superfamily of acute phase reactants charactarized by a pentameric structure· The C-reactive protein (CRP) and serum amyloid P-component (SAP) are wellcharactarized short Pentraxins, which are produced in the liver in response to inflammatory mediators· Pentraxin 3 (PTX3), also known as Tumor necrosis factor-Stimulated Gene 14 (TSG-14), is the prototypic long Pentraxin, with a high degree of conservation from mouse to man· Human PTX3 encodes a 45 kDa secretory glycoprotein with a 162 amino acid N-terminal extension and a 202 amino acid C-terminal Pentraxin domain· The protein is locally produced and released by a variety of cell types including macrophages, neutrophils, myeloid-derived mesangial cells, synovial cells, smooth muscle cells, alveolar epithelium, and glial cells· PTX3 is induced in response to either inflammatory cytokines interleukin-1 β (IL-1 β) and tumor necrosis factor α (TNFα) or the selected associated molecular patterns (PAMPs)· PTX3 has a dual role in the regulation of the innate immune response· Via its C-terminal Pentraxin domain, immobilized PTX3 will bind complement component C1q to induce classical complement activation· However, fluid-phase PTX3 inhibits classical complement activation· Furthermore PTX3 participates in the clearance of apoptic cells· PTX3 is elevated in critically ill patients, with a gradient from systematic inflammatory resonse syndrome to septic shock, and in several other diseases, such as mycocardial infarction, rheumatoid arthritis, atherosclerosis, small vessel vasculitis and psoriasis· Furthermore serum levels of PTX3 are higher in preeclamsia compared with normal pregancies··

产品特点：The human pentraxin 3 ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3·5 hours· The efficient format of 2 plates with twelve disposable 8-well strips allows free choice of batch size for the assay· Samples and standards are captured by a solid bound specific antibody· Biotinylated tracer antibody will bind to captured human pentraxin 3· Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody· Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB)· The enzyme reaction is stopped by the addition of citric acid· The absorbance at 450 nm is measured with a spectrophotometer· A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human pentraxin 3 standards (log)· The human pentraxin 3 concentration of samples, which are run concurrently with the standards, can be determined from the standard curve·

保存建议：推荐4℃保存，有效期一年。

定购信息： 

产品名称	规格	操作手册	货号	询价
穿透素3(人)ELISA试剂盒	1 x 96 det	PDF	E-HK347-01
穿透素3(人)ELISA试剂盒	2 x 96 det	PDF	E-HK347-02
VisionBlue快速细胞活力分析试剂盒	500 次	PDF	C-K303-500

表观遗传学产品全面解决方案列下：

产品名称	规格	操作手册	货号	询价
DNA羟甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1037-48
DNA羟甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1037-96
DNA羟甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1036-48
DNA羟甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1036-96
DNA甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1035-48
DNA甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1035-96
DNA甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1034-48
DNA甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1034-96
通用DNA甲基化试剂盒(定性分析)	48 次	PDF	A-P-1011-48
通用DNA甲基化试剂盒(定性分析)	96 次	PDF	A-P-1011-96
超敏DNA甲基化定量试剂盒	48 次	PDF	A-P-1021-48
超敏DNA甲基化定量试剂盒	96 次	PDF	A-P-1021-96
总DNA甲基化定量试剂盒	48 次	PDF	A-P-1014-48
总DNA甲基化定量试剂盒	96 次	PDF	A-P-1014-96

推荐阅读：

关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC

5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶，并且区分5-hmC, 5-mC, 和 C，使得研究者能够重新评估他们的DNA甲基化信息，也能够在新样品中寻找DNA羟甲基化。