C5a(人)ELISA试剂盒 货号：E-HK349
C5a, Human, ELISA kit
背景资料：The human C5a ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3·5 hours· The efficient format of 2 plates with twelve disposable 8-well strips allows free choice of batch size for the assay· Samples and standards are incubated in microtiter wells coated with antibodies recognizing human C5a· Biotinylated tracer antibody will bind to the captured human C5a· Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody· Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB)· The enzyme reaction is stopped by the addition of oxalic acid· The absorbance at 450 nm is measured with a spectrophotometer· A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human C5a standards (log)· The human C5a concentration of samples, which are run concurrently with the standards, can be determined from the standard curve··
产品描述：The complement system is an important factor in innate immunity· During complement activation of complement protein 5 (C5), C5 is proteolytically cleaved and the anaphylatoxic peptide C5a is generated· C5a is a small polypeptide consisting of 74 aminoacids (11kDa) and is derived from the N-terminus of the alpha chain· C5a itself is very short-lived and in serum is cleaved rapidly into the more stable, though biologically still active C5a-desArg (also called acylation stimulating protein, ASP· Therefore, measurement of C5a-desArg allows reliable conclusions about the level of complement activation in the test samples· For convenience, both forms will be referred to in the following text as C5a· C5a acts as a potent anaphylatoxin causing smooth muscle contraction, vasodilation, increased vascular permeability, basophil and mast cell degranulation and lysosomal enzyme release· In addition, C5a is a potent chemotactic factor for eosinophils, basophils and monocytes·
产品特点：Human C5a can be measured accurately if serum or plasma samples are diluted at least 4x and For activated serum human C5a can be measured accurately if serum samples are diluted at least 100x·Human C5a can be measured accurately if CSF samples are diluted at least 2x and if sputum samples are diluted at least 2x · The cross reactivity with Human C3a was negative·
保存建议：推荐4℃保存，有效期一年。
定购信息：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| C5a(人)ELISA试剂盒 |
1 x 96 det |
PDF |
E-HK349-01 |
 |
| C5a(人)ELISA试剂盒 |
2 x 96 det |
PDF |
E-HK349-02 |
|
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