L-FABP(人)ELISA试剂盒                                           货号：E-HK404

L-FABP, Human, ELISA kit

背景资料：The human L-FABP ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3·5 hours· The efficient format of 2 plates with twelve disposable 8-well strips allows free choice of batch size for the assay· Samples and standards are incubated in microtiter wells coated with antibodies recognizing human L-FABP· Biotinylated tracer antibody will bind to captured human L-FABP· Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody· Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB)· The enzyme reaction is stopped by the addition of citric acid· The absorbance at 450 nm is measured with a spectrophotometer· A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human L-FABP standards (log)· The human L-FABP concentration of samples, which are run concurrently with the standards, can be determined from the standard curve·

产品描述：Fatty acid-binding proteins (FABPs) are a class of cytoplasmic proteins that bind long chain fatty acids· FABPs are small intracellular proteins (~13-14 kDa) with a high degree of tissue specificity· They are abundantly present in various cell types and play an important role in the intracellular utilization of fatty acids, transport and metabolism· There are at least nine distinct types of FABP, each showing a specific pattern of tissue expression· Due to its small size, FABP leaks rapidly out of ischemically damaged necrotic cells leading to a rise in serum levels· Ischemically damaged tissues are characterized histologically by absence (or low presence) of FABP facilitating recognition of such areas· Liver-type fatty acid binding protein (L-FABP, FABP1) is predominantly expressed in liver· The L-FABP protein is derived from the human FABP1 gene· L-FABP is a sensitive marker for cell damage of liver cells in vitro and in vivo· L-FABP is also a marker for rapid hepatocyte lysis in vitro (as for example in toxicology assays) and for detection of liver damage during and after transplantation· Serum/plasma and urine of healthy individuals contains approximately 12 ng/ml and 16 ng/ml L-FABP, respectively·

产品特点：The human L-FABP ELISA has been developed for the quantitative measurement of natural and recombinant human L-FABP in cell culture medium, serum, plasma and urine· In serum or plasma samples human L-FABP can be measured accurately if serum or plasma samples are diluted at least 20 times· The cross reactivity with Human H-FABP and Human I-FABP was negative·.

保存建议：推荐4℃保存，有效期一年。

定购信息： 

产品名称	规格	操作手册	货号	询价
L-FABP(人)ELISA试剂盒	1 x 96 det	PDF	E-HK404-01
L-FABP(人)ELISA试剂盒	2 x 96 det	PDF	E-HK404-02
VisionBlue快速细胞活力分析试剂盒	500 次	PDF	C-K303-500

表观遗传学产品全面解决方案列下：

产品名称	规格	操作手册	货号	询价
DNA羟甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1037-48
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DNA羟甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1036-48
DNA羟甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1036-96
DNA甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1035-48
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DNA甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1034-48
DNA甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1034-96
通用DNA甲基化试剂盒(定性分析)	48 次	PDF	A-P-1011-48
通用DNA甲基化试剂盒(定性分析)	96 次	PDF	A-P-1011-96
超敏DNA甲基化定量试剂盒	48 次	PDF	A-P-1021-48
超敏DNA甲基化定量试剂盒	96 次	PDF	A-P-1021-96
总DNA甲基化定量试剂盒	48 次	PDF	A-P-1014-48
总DNA甲基化定量试剂盒	96 次	PDF	A-P-1014-96

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