I-FABP(人)ELISA试剂盒 货号：E-HK406
I-FABP, Human, ELISA kit
背景资料：The human intestinal FABP ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3·5 hours· The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay· Samples and standards are incubated in microtiter wells coated with antibo璬ies recognizing human I-FABP Biotinylated tracer antibody will bind to captured human I-FABP· Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody· Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB)· The enzyme reaction is stopped by the addition of citric acid· The absorbance at 450 nm is measured with a spectrophotometer· A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human I-FABP standards (log)· The human I-FABP concentration of samples, which are run concurrently with the standards, can be determined from the standard curve··
产品描述：Fatty acid-binding proteins (FABPs) are a class of cytoplasmic proteins that bind long chain fatty acids· FABPs are small intracellular proteins (~13-14 kDa) with a high degree of tissue specificity· They are abundantly present in various cell types and play an important role in the intracellular utilization of fatty acids, transport and metabolism· There are at least nine distinct types of FABP, each showing a specific pattern of tissue expression· Due to its small size, FABP leaks rapidly out of ischemically damaged necrotic cells leading to a rise in serum levels· Ischemically damaged tissues are characterized histologically by absence (or low presence) of FABP facilitating recognition of such areas· Intestinal FABP (I-FABP) is specifically localized in the epithelium cells of the small bowel· The I-FABP protein is derived from the human FABP2 gene· Normally, I-FABP is undetectable in serum· Many observations indicate that I-FABP is a useful biochemical marker for intestinal cell damage both in vivo and in vitro· Ischemically damaged cells are characterized histologically by absence (or low presence) of FABP facilitating recognition of areas of ischemically damaged cells··
产品特点：The human I-FABP ELISA kit is intended for the quantitative measure璵ent of human I-FABP in cell culture medium, serum, plasma and urine· In serum or plasma samples, human I-FABP can be measured accurately if serum or plasma samples are diluted at least 2 times·
保存建议：推荐4℃保存，有效期一年。
定购信息：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| I-FABP(人)ELISA试剂盒 |
1 x 96 det |
PDF |
E-HK406-01 |
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| I-FABP(人)ELISA试剂盒 |
2 x 96 det |
PDF |
E-HK406-02 |
|
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