H-FABP(大鼠)ELISA试剂盒                                           货号：E-HK414

H-FABP, Rat, ELISA kit

背景资料：The rat H-FABP ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 2·5 hours for Rat H-FABP· The efficient format of 2 plates with twelve disposable 8-well strips allows free choice of batch size for the assay· Samples and standards are captured by a solid bound specific antibody· Biotinylated tracer antibody will bind to captured rat H-FABP· Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody· Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB)· The enzyme reaction is stopped by the addition of citric acid· The absorbance at 450 nm is measured with a spectrophotometer· A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the mouse/rat H-FABP standards (log)· The H-FABP concentration of samples, which are run concurrently with the standards, can be determined from the standard curve··

产品描述：Fatty acid-binding proteins (FABPs) are a class of cytoplasmic proteins that bind long chain fatty acids· FABPs are small intracellular proteins (~13-14 kDa) with a high degree of tissue specificity· They are abundantly present in various cell types and play an important role in the intracellular utilization of fatty acids, transport and metabolism· There are at least nine distinct types of FABP, each showing a specific pattern of tissue expression· Due to its small size, FABP leaks rapidly out of ischemically damaged necrotic cells leading to a rise in serum levels· Ischemically damaged tissues are characterized histologically by absence (or low presence) of FABP facilitating recognition of such areas· The H-FABP protein is derived from the FABP3 gene· The FABP content of rat heart muscle (H-FABP) is markedly high, 10-20 mol% of cytoplasmic proteins· Following acute myocardial infarction (AMI) the small protein H-FABP is rapidly released in to the circulation· Significantly elevated serum/plasma concentrations are found within 3 h after AMI which generally return to normal values within 12 to 24 h· These features make H-FABP a useful research tool for the early assessment or exclusion of AMI, and for the monitoring of a recurrent infarction· Constitutive H-FABP released from the heart after AMI is quantitatively recovered in serum/plasma· Thus assessment of H-FABP is also a very effective tool for the estimation of the infarct size· The Rat H-FABP kit can also be used for measurement of brain-type FABP, a marker for brain injury detection· In serum/plasma of healthy individuals approximately 1·6 ng/ml of H-FABP is present·

产品特点：The rat H-FABP ELISA has been developed for the quantitative measurement of natural rat heart FABP in serum and plasma· The ELISA can be used to detect heart damage· In serum or plasma samples, rat H-FABP can be measured accurately if serum or plasma samples are diluted at least 2 times·

保存建议：推荐4℃保存，有效期一年。

定购信息： 

产品名称	规格	操作手册	货号	询价
H-FABP(大鼠)ELISA试剂盒	1 x 96 det	PDF	E-HK414-01
H-FABP(大鼠)ELISA试剂盒	2 x 96 det	PDF	E-HK414-02
VisionBlue快速细胞活力分析试剂盒	500 次	PDF	C-K303-500

表观遗传学产品全面解决方案列下：

产品名称	规格	操作手册	货号	询价
DNA羟甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1037-48
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DNA羟甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1036-48
DNA羟甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1036-96
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DNA甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1034-48
DNA甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1034-96
通用DNA甲基化试剂盒(定性分析)	48 次	PDF	A-P-1011-48
通用DNA甲基化试剂盒(定性分析)	96 次	PDF	A-P-1011-96
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超敏DNA甲基化定量试剂盒	96 次	PDF	A-P-1021-96
总DNA甲基化定量试剂盒	48 次	PDF	A-P-1014-48
总DNA甲基化定量试剂盒	96 次	PDF	A-P-1014-96

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