LBP ELISA试剂盒 货号：E-HK503
LBP, various species, ELISA kit
背景资料：The LBP ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3·5 hours· The efficient format of 1 plate with twelve disposable 8-well strips allows free choice of batch size for the assay· Samples and standards are incubated in microtiter wells coated with antibo璬ies recognizing LBP· Biotinylated LPS will bind to captured LBP· Streptavidin-peroxidase conjugate will bind to the biotinylated LPS· Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB)· The enzyme reaction is stopped by the addition of citric acid· The absorbance at 450 nm is measured with a spectrophotometer· A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the LBP standards (log)· The LBP concentration of samples, which are run concurrently with the human LBP standards, can be determined from the human LBP standard curve··
产品描述：Lipopolysacharide (LPS) Binding Protein (LBP) is a type 1 acute phase protein that is constitutively produced by the liver and rapidly upregulated during the acute phase response· LBP plays a central role in the response to LPS· The protein catalyses the monomerization of LPS and its transfer to (s)CD14 and to lipoproteins· In this way LBP has both a role in the activation pathway of LPS: activation of monocytes by LPS leading to release of inflammatory mediators and in the neutralization of LPS i·e· the uptake of LPS by lipoprotein and subsequent clearing· The assay can be used to quantify functional LBP in tissue culture supernatants of cell cultures, plasma and serum samples of several species· In plasma of healthy individuals LBP is present at levels which increase during acute phase responses·
产品特点：The LBP (lipopolysaccharide binding protein) kit is intended for the relative quantitative measure璵ent of natural and recombinant LBP of a wide variety of species in cell culture medium, plasma or serum· Measurable species are: bovine, canine, human, monkey, rabbit, rat, sheep and swine· For quantification human LBP is used as reference standard· In serum or plasma samples LBP can be measured accurately if serum or plasma samples are diluted at least 3 times; dilution depends on the species measured·
保存建议：推荐4℃保存，有效期一年。
定购信息：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| LBP ELISA试剂盒 |
1 x 96 det |
PDF |
E-HK503 |
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