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PfuTurbo Cx Hotstart DNA Polymerase 货号：A-600412
PfuTurbo Cx Hotstart DNA Polymerase
背景资料：Deamination of cytosine to uracil has been shown to occur during PCR and to impact the performance of proofreading archaeal DNA polymerases by a phenomenon known as PCR poisoning. Cytosine deamination at elevated temperatures can occur either in the nucleotide pool (dCTP®dUTP) or in the template strand (dCMP®dUMP). The dUTP that arises during PCR is efficiently incorporated by archaeal DNA polymerases (e.g., Pfu DNA polymerase, Vent DNA polymerase, Tgo DNA polymerase). However, this group of polymerases also exhibits the unique tendency of stalling replication opposite uracil in the template strand, thereby limiting PCR product yield. Unlike dUTP incorporation opposite dAMP, which is a non-mutagenic event (A/T®A/U), deamination of dCMP to dUMP in DNA is a major promutagenic event, generating G/C®A/T mutations if not repaired before DNA replication. Although uracil stalling limits PCR product yield, this unique property is expected to contribute to the high fidelity of Pfu DNA polymerase.
产品描述：The PfuTurbo Cx hotstart DNA polymerase is formulated with a mutant of Pfu DNA polymerase that overcomes uracil stalling completely, allowing the polymerase to read through uracil located in the template strand or incorporated into the extending strand. Since uracil read-through can introduce mutations that adversely impact fidelity, we have further engineered the mutant polymerase to increase the level of proofreading activity, thereby reducing the error rate of PfuTurbo Cx hotstart DNA polymerase to that of the original PfuTurbo DNA polymerase. (See Table I for a comparison of the fidelity characteristics of many commercially available DNA polymerases.) Thus PfuTurbo Cx hotstart DNA polymerase improves the overall reliability of high-fidelity PCR and exhibits more robust performance, affording higher product yields from templates of 5–10 kb in length as well as from more difficult systems including targets with high GC-content, without sacrificing accuracy. Although most systems amplify successfully without the need for additives, DMSO is included with PfuTurbo Cx hotstart DNA polymerase to further enhance yields of long, high-complexity, or GC-rich target sequences.
产品特点：
1· World’s only high-fidelity polymerase that efficiently reads through and incorporates dUTP；
2·Ideal for bi-sulfite sequencing, UNG decontamination protocols, and DNA methylation studies；
3·Amplifies 5-10 kb targets with high GC content；
4.DMSO provided separately to assist in optimizing PCR conditions。
保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分
定购信息：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| PfuTurbo Cx Hotstart DNA Polymerase |
1000 U |
PDF |
A-600414 |
 |
| PfuTurbo Cx Hotstart DNA Polymerase |
500 U |
PDF |
A-600412 |
|
| PfuTurbo Cx Hotstart DNA Polymerase |
100 U |
PDF |
A-600410 |
|
| Paq5000 Hotstart PCR Master Mix |
100 次 |
PDF |
A-600870 |
|
| 植物DNA小量提取试剂盒 |
50 次 |
PDF |
A-DH813-01 |
|
| 植物DNA小量提取试剂盒 |
200 次 |
PDF |
A-DH813-02 |
|
| 凝胶DNA纯化回收试剂盒 |
100 次 |
PDF |
A-D2110-01 |
|
| 凝胶DNA纯化回收试剂盒 |
200 次 |
PDF |
A-D2110-02 |
|
| 凝胶DNA纯化回收试剂盒 |
500 次 |
PDF |
A-D2110-03 |
|
| 凝胶DNA纯化回收试剂盒 |
1000次 |
PDF |
A-D2110-04 |
|
| 胶自动纯化回收试剂盒(磁珠法) |
150 次 |
PDF |
A-DM136-01 |
|
| 胶自动纯化回收试剂盒(磁珠法) |
500 次 |
PDF |
A-DM136-02 |
|
| 总RNA自动化富集纯化mRNA试剂盒(磁珠法) |
30 次 |
PDF |
A-RM911-01 |
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| 植物自动化DNA大量提取试剂盒(磁珠法) |
50 次 |
PDF |
A-DM311-01 |
|
| 组织切片DNA极速提取试剂盒 |
100 次 |
PDF |
A-P-1003-100 |
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表观遗传学产品全面解决方案列下：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| DNA羟甲基化定量试剂盒(荧光法) |
48 次 |
PDF |
A-P-1037-48 |
|
| DNA羟甲基化定量试剂盒(荧光法) |
96 次 |
PDF |
A-P-1037-96 |
|
| DNA羟甲基化定量试剂盒(比色法) |
48 次 |
PDF |
A-P-1036-48 |
|
| DNA羟甲基化定量试剂盒(比色法) |
96 次 |
PDF |
A-P-1036-96 |
|
| DNA甲基化定量试剂盒(荧光法) |
48 次 |
PDF |
A-P-1035-48 |
|
| DNA甲基化定量试剂盒(荧光法) |
96 次 |
PDF |
A-P-1035-96 |
|
| DNA甲基化定量试剂盒(比色法) |
48 次 |
PDF |
A-P-1034-48 |
|
| DNA甲基化定量试剂盒(比色法) |
96 次 |
PDF |
A-P-1034-96 |
|
| 通用DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1011-48 |
|
| 通用DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1011-96 |
|
| 超敏DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1021-48 |
|
| 超敏DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1021-96 |
|
| 总DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1014-48 |
|
| 总DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1014-96 |
|
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