组蛋白H4多通道修饰分析试剂盒(比色法)                                                                                            货号：A-P-3102

EpiQuik Histone H4 Modification Multiplex Assay Kit (Colorimetric)

背景资料：Histone modifications have been defined as epigenetic modifiers. Post-translational modifications of histones include the acetylation of specific lysine residues by histone acetyltransferases (HATs), deacetylation by histone deacetylase (HDACs), the methylation of lysine and arginine residues by histone methytransferases (HMTs), the demethylation of lysine residues by histone demethylases (HDMTs), and the phosphorylation of specific serine groups by histone kinases (HKs). Additional histone modifications include the attachment of ubiquitin (Ub), small ubiquitin-like modifiers (SUMOs), and poly ADP-ribose (PAR) units. Next to DNA methylation, histone acetylation and histone methylation are the most well characterized epigenetic marks. There are many known sites and types of post-translational modification on human histone H4 that include Ser-1 phosphorylation, Arg-3 methylation, lysine 5, 8, 12, and 16 acetylation and lysine 20 mono-, di-, and trimethylation. Generally, euchromatin is characterized by a high level of histone H4 acetylation, which is mediated by histone acetyltransferases. Histone deacetylases have the ability to remove this epigenetic mark, which leads to transcriptional repression. Condensed heterochromatin is enriched in methylation of H4K20. Lysine residues of histone H4 can be mono-, di-, or trimethylated, each of which can differentially regulate chromatin structure and transcription. Along with other histone modifications such as phosphorylation, this enormous variation leads to a multiplicity of possible combinations of different modifications. This may constitute a “histone code”, which can be read and interpreted by different cellular factors.

产品描述：The EpiQuik™ Histone H4 Modification Multiplex Assay Kit (Colorimetric) is designed for measuring multiple histone H4 modifications simultaneously. In an assay with this kit, each histone H4 modified at specific sites will be captured by an antibody that is coated on the strip wells and specifically targets the appropriate histone H4 modification pattern. The captured histone modified at specific sites will be detected with a detection antibody, followed by a color development reagent. The ratio of modified histone is proportional to the intensity of absorbance measured by a microplate reader at a wavelength of 450 nm。  

产品特点：

1·Simultaneously measure 10 different histone H4 modifications, which includes nearly all modified histone H4 sites. ；

2·Quick and efficient procedure, which can be finished within 2 hours and 30 minutes；

3·Innovative colorimetric assay without the need for radioactivity, electrophoresis, chromatography, or expensive equipment；

4.High sensitivity with a detection limit as low as 0.5 ng/well for each modification pattern and a detection range from 20 ng to 500 ng/well of histone extracts；

5.An assay control is conveniently included for quantification of each histone H4 modification；

6.Total histone H4 sets are included, which can be used for normalizing total histone H4 levels for relative comparison of histone H4 content between different samples or different treatment conditions ；

7.Strip-well microplate format makes the assay flexible: manual or high throughput, which enables analysis of a single modification or a total of 10 modification patterns within the same samples；

8.Two extra 8-well strips are included in the kit which can be used, if necessary, for sample amount pre-optimization to determine the input amount (ex: 50, 100, 200 ng/well) needed to fall within the detection limits of the assay. Extra strips may also be used as assay controls and total histone level controls if selective detection of some histone H4 modifications from the total 21 modification pattern is desired；

9.Simple, reliable, and consistent assay conditions。

保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分

定购信息：

产品名称	规格	操作手册	货号	询价
组蛋白H4多通道修饰分析试剂盒(比色法)	96 次	PDF	A-P-3102-96
高敏免疫沉淀试剂盒	48 次	PDF	A-P-2027-48
高敏免疫测序试剂盒	12 次	PDF	A-P-2030-12
高敏免疫测序试剂盒	24 次	PDF	A-P-2030-24
RNA甲基化修饰试剂盒	50 次	PDF	A-P-9003-050
RNA甲基化修饰试剂盒	200 次	PDF	A-P-9003-200
亚硫酸盐快速DNA文库制备试剂盒(illumina)	12 次	PDF	A-P-1055-12
亚硫酸盐快速DNA文库制备试剂盒(illumina)	24 次	PDF	A-P-1055-24
高敏感亚硫酸盐测序试剂盒(illumina)	12 次	PDF	A-P-1056-12
高敏感亚硫酸盐测序试剂盒(illumina)	24 次	PDF	A-P-1056-24
SETDB1特异基因敲除定量试剂盒-表观遗传调控因子	96 次	PDF	A-P-5002-SETDB1-96
通用乙酰化组蛋白H3K36定量分析试剂盒(荧光法)	96 次	PDF	A-P-4019-96

表观遗传学产品全面解决方案列下：

产品名称	规格	操作手册	货号	询价
DNA羟甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1037-48
DNA羟甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1037-96
DNA羟甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1036-48
DNA羟甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1036-96
DNA甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1035-48
DNA甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1035-96
DNA甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1034-48
DNA甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1034-96
通用DNA甲基化试剂盒(定性分析)	48 次	PDF	A-P-1011-48
通用DNA甲基化试剂盒(定性分析)	96 次	PDF	A-P-1011-96
超敏DNA甲基化定量试剂盒	48 次	PDF	A-P-1021-48
超敏DNA甲基化定量试剂盒	96 次	PDF	A-P-1021-96
总DNA甲基化定量试剂盒	48 次	PDF	A-P-1014-48
总DNA甲基化定量试剂盒	96 次	PDF	A-P-1014-96

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