组蛋白H3多通道修饰分析试剂盒(比色法)                                                                                            货号：A-P-3100

EpiQuik Histone H3 Modification Multiplex Assay Kit (Colorimetric)

背景资料：Histone modifications have been defined as epigenetic modifiers. Post-translational modifications of histones include the acetylation of specific lysine residues by histone acetyltransferases (HATs), deacetylation by histone deacetylase (HDACs), the methylation of lysine and arginine residues by histone methytransferases (HMTs), the demethylation of lysine residues by histone demethylases (HDMTs), and the phosphorylation of specific serine groups by histone kinases (HKs). Additional histone modifications include the attachment of ubiquitin (Ub), small ubiquitin-like modifiers (SUMOs), and poly ADP-ribose (PAR) units. Next to DNA methylation, histone acetylation and histone methylation are the most well characterized epigenetic marks. Generally, tri-methylation at H3-K4, H3-K36, or H3-K79 results in an open chromatin configuration and is therefore characteristic of euchromatin. Euchromatin is also characterized by a high level of histone acetylation, which is mediated by histone acetyltransferases. Lysine residues can be mono-, di-, or tri-methylated, each of which can differentially regulate chromatin structure and transcription. Along with other histone modifications such as phosphorylation, this enormous variation leads to a multiplicity of possible combinations of different modifications. This may constitute a “histone code”, which can be read and interpreted by different cellular factors.

产品描述：In an assay with this kit, each histone H3 modified at specific sites will be captured by an antibody that is coated on the strip wells and specifically targets the appropriate histone modification pattern. The captured histone modified at specific sites will be detected with a detection antibody, followed by a color development reagent. The ratio of modified histone is proportional to the intensity of absorbance measured by a microplate reader at a wavelength of 450 nm。  

产品特点：

1·Simultaneously measure 21 different histone H3 modifications, which includes all of the most important and the most well-characterized patterns

2·Quick and efficient procedure, which can be finished within 2 hours and 30 minutes；

3·Innovative colorimetric assay without the need for radioactivity, electrophoresis, chromatography, or expensive equipment；

4.High sensitivity with a detection limit as low as 0.5 ng/well for each modification pattern and a detection range from 20 ng to 500 ng/well of histone extracts；

5.An assay control is conveniently included for quantification of each histone H3 modification；

6.Total histone H3 sets are included, which can be used for normalizing total histone H3 levels for relative comparison of histone H3 content between different samples or different treatment conditions ；

7.Strip microplate format makes the assay flexible: manual or high throughput, which enables analysis of a single modification or total 21 modification patterns within the same samples；

8.Two extra 8-well strips are included in the kit which can be used, if necessary, for sample amount pre-optimization to determine the input amount (ex: 50, 100, 200 ng/well) needed to fall within the detection limits of the assay. Extra strips may also be used as assay controls and total histone level controls if selective detection of some histone H3 modifications from the total 21 modification pattern is desired；

9.Simple, reliable, and consistent assay conditions。

保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分

定购信息：

产品名称	规格	操作手册	货号	询价
组蛋白H3多通道修饰分析试剂盒(比色法)	96 次	PDF	A-P-3100-96
高敏免疫沉淀试剂盒	48 次	PDF	A-P-2027-48
高敏免疫测序试剂盒	12 次	PDF	A-P-2030-12
高敏免疫测序试剂盒	24 次	PDF	A-P-2030-24
RNA甲基化修饰试剂盒	50 次	PDF	A-P-9003-050
RNA甲基化修饰试剂盒	200 次	PDF	A-P-9003-200
亚硫酸盐快速DNA文库制备试剂盒(illumina)	12 次	PDF	A-P-1055-12
亚硫酸盐快速DNA文库制备试剂盒(illumina)	24 次	PDF	A-P-1055-24
高敏感亚硫酸盐测序试剂盒(illumina)	12 次	PDF	A-P-1056-12
高敏感亚硫酸盐测序试剂盒(illumina)	24 次	PDF	A-P-1056-24
SETDB1特异基因敲除定量试剂盒-表观遗传调控因子	96 次	PDF	A-P-5002-SETDB1-96
通用乙酰化组蛋白H3K36定量分析试剂盒(荧光法)	96 次	PDF	A-P-4019-96

表观遗传学产品全面解决方案列下：

产品名称	规格	操作手册	货号	询价
DNA羟甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1037-48
DNA羟甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1037-96
DNA羟甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1036-48
DNA羟甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1036-96
DNA甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1035-48
DNA甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1035-96
DNA甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1034-48
DNA甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1034-96
通用DNA甲基化试剂盒(定性分析)	48 次	PDF	A-P-1011-48
通用DNA甲基化试剂盒(定性分析)	96 次	PDF	A-P-1011-96
超敏DNA甲基化定量试剂盒	48 次	PDF	A-P-1021-48
超敏DNA甲基化定量试剂盒	96 次	PDF	A-P-1021-96
总DNA甲基化定量试剂盒	48 次	PDF	A-P-1014-48
总DNA甲基化定量试剂盒	96 次	PDF	A-P-1014-96

推荐阅读：

关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC

5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶，并且区分5-hmC, 5-mC, 和 C，使得研究者能够重新评估他们的DNA甲基化信息，也能够在新样品中寻找DNA羟甲基化。