 |
Epigenetics |
 |
|
|
|
|
|
 |
| Epigenetics>【BioLegend】 |
| |
|
| |
| |
Methylated & Non-methylated pUC19 DNA Set™(A-D5017) |
| |
|
|
|
Methylated & Non-methylated pUC19 DNA Set™ 货号：A-D5017
背景资料：The Methylated & Non-methylated pUC19 DNA Set consists of two control DNAs (methylated and non-methylated) along with a set of specifically designed primers that can be used in conjunction with the EZ DNA Methylation™, EZ DNA Methylation-Gold™, and EZ DNA Methylation-Direct™ kits from Zymo Research to assess the efficiency of bisulfite-mediated conversion of DNA. These plasmids can be used in conjunction with genomic DNAs to provide internal controls to assess bisulfite conversion efficiency or to produce known mixtures of methylated and non-methylated DNA for assay calibration.
The Non-Methylated pUC19 DNA is pUC19 that was isolated from a methylation-negative strain of bacteria (Dam¯, Dcm¯) and can be used as a negative control for DNA methylation analysis. The Methylated pUC19 DNA is pUC19 that has been isolated from the same strain and has been enzymatically methylated at all cytosine positions comprising CG dinucleotides by M.SssI methyltransferase2 (EC 2.1.1.37; Figure 1) and can be used as a positive control for DNA methylation analysis.
产品特点：
|
Storage
|
Reagents in this kit are stored at - 20°C and are stable for 1 year. |
|
Transformation Efficiency
|
The primer set herein has been designed to amplify a fragment of the supplied pUC19 DNAs following bisulfite treatment. The methylated cytosines, comprising CG dinucleotides in the Methylated pUC19 DNA remain unconverted following bisulfite treatment, whereas nonmethylated cytosines are converted into uracil and detected as thymine after PCR. The supplied pUC19 DNA has been linearized at position 2177 using ScaI endonuclease.
|
保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分
定购信息：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| Methylated & Non-methylated pUC19 DNA Set™ |
1 set |
PDF |
A-D5017 |
 |
| Zymolyase - Yeast Lytic Enzyme |
2000 Units |
PDF |
A-E1005 |
|
| 细菌基因组DNA极速提取试剂盒（离心柱） |
200次 |
PDF |
A-D2001-200 |
|
表观遗传学产品全面解决方案列下：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| DNA羟甲基化定量试剂盒(荧光法) |
48 次 |
PDF |
A-P-1037-48 |
|
| DNA羟甲基化定量试剂盒(荧光法) |
96 次 |
PDF |
A-P-1037-96 |
|
| DNA羟甲基化定量试剂盒(比色法) |
48 次 |
PDF |
A-P-1036-48 |
|
| DNA羟甲基化定量试剂盒(比色法) |
96 次 |
PDF |
A-P-1036-96 |
|
| DNA甲基化定量试剂盒(荧光法) |
48 次 |
PDF |
A-P-1035-48 |
|
| DNA甲基化定量试剂盒(荧光法) |
96 次 |
PDF |
A-P-1035-96 |
|
| DNA甲基化定量试剂盒(比色法) |
48 次 |
PDF |
A-P-1034-48 |
|
| DNA甲基化定量试剂盒(比色法) |
96 次 |
PDF |
A-P-1034-96 |
|
| 通用DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1011-48 |
|
| 通用DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1011-96 |
|
| 超敏DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1021-48 |
|
| 超敏DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1021-96 |
|
| 总DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1014-48 |
|
| 总DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1014-96 |
|
推荐阅读：
关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPI研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶，并且区分5-hmC, 5-mC, 和 C，使得研究者能够重新评估他们的DNA甲基化信息，也能够在新样品中寻找DNA羟甲基化。
|
| |
|
| |
|
|
| |
|
