NOMe-Seq Kit(核小体和DNA甲基化定位试剂盒) 货号：A-54000
NOMe-Seq Kit
背景资料：The NOMe-Seq assay works by treating fixed chromatin with a GpC methyltransferase enzyme to artificially methylate GpC dinucleotides that are not protected by nucleosomes or other proteins bound to the DNA. Due to the high abundance of GpC residues within the genome, the enzymatic treatment provides a high resolution footprint of nucleosome occupancy. Treated DNA is then subjected to bisulfite conversion and gene-specific loci are sequenced to establish the DNA methylation profile for a single DNA strand. Since GpC dinucleotides are not methylated in mammalian genomes, the endogenous CpG methylation can be distinguished from the artificially introduced GpC methylation. By combining the sequencing information for the GpC sites with the CpG sites, a profile of nucleosome occupancy and DNA methylation for a specific gene locus can be determined.
产品描述：NOMe-Seq stands for Nucleosome Occupancy and Methylome Sequencing. It is a technique that was developed by the Peter A. Jones lab at USC that allows researchers to interrogate the relationship between nucleosome occupancy, transcription factor binding and DNA methylation for specific gene loci.11-14 NOMe-Seq works by treating fixed chromatin with a GpC methyltransferase enzyme to artificially methylate GpC dinucleotides that are not protected by nucleosome or protein binding sites. The high abundance of GpC dinucleotides within the genome allows for better resolution of the nucleosome footprint such that even subtle changes in nucleosome position can be detected. Extracted DNA is then treated with sodium bisulfite to convert unmethylated cytosines into uracil while leaving methylated cytosine residues as cytosine. Following cloning and sequencing, the results can be mapped to compare the artificially methylated GpC and the endogenously methylated CpG residues and establish a temporal relationship between nucleosome occupancy and endogenous DNA methylation.
产品特点：
1·同时分析同一条DNA链上的核小体定位和CpG 甲基化；
2·了解DNA甲基化与核小体占位之间的瞬时关系；
3.足够的灵敏度可检测核小体位置上微妙的变化；
4.核酸酶消化分析表明无核小体占位偏向；
5.染色质固定也提供了转录因子结合位点的信息。
保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分。
定购信息：
| 产品名称 |
规格 |
操作手册 |
货号 |
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10 次 |
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