EpiNext RNA 亚硫酸盐-测序 试剂盒 货号：A-P-9006
EpiNext RNA Bisulfite-Seq Kit (Illumina)
背景资料：It has been observed that in humans, 5-methylcytosine occurs in various RNA molecules including tRNAs, rRNAs, mRNAs and non-coding RNAs (ncRNAs). At least 10,275 5-mC candidate sites were discovered in mRNAs and ncRNAs, which cover 10.6% of the total cytosine residues in the transcriptome. 5-mC seems to be enriched in some classes of ncRNA, but relatively depleted in mRNAs. However, the majority (83%) of their candidate sites were found in mRNAs. Within these transcripts 5-mC appears to be depleted within protein coding sequences but enriched in 5’ and 3’ UTRs. Two different methyltransferases, NSUN2 and DNMT2 are known to catalyze 5-mC modification in eukaryotic RNA. Recent data strongly suggest that RNA cytosine methylation affects the regulation of various biological processes such as RNA stability and mRNA translation. Furthermore, loss of 5-mC in vault RNAs causes aberrant processing into Argonaute-associated small RNA fragments that can function as microRNAs. Thus, impaired processing of vault ncRNA may contribute to the etiology of human disorders related to NSun2-deficiency. Bisulfite conversion of RNA followed by next generation sequencing yields reliable information about RNA cytosine methylation states on a transcriptome-wide scale.
产品描述：The EpiNext™ RNA Bisulfite-Seq Kit (Illumina) is a complete set of optimized reagents designed to carry out RNA bisulfite conversion, followed by a "post-bisulfite" library preparation process for Illumina platform-based bisulfite sequencing, all in one kit. Intended applications include whole transcriptome RNA bisulfite sequencing and various other RNA bisulfite-based next generation sequencing techniques for RNA methylation analysis. The optimized protocol and components of the kit allow the RNA to be bisulfite converted and fragmented simultaneously followed by quick non-barcoded (singleplexed) and barcoded (mutilplexed) library construction using low-nanogram quantities of bisulfite converted RNA.
产品特点：
1· Fast and streamlined procedure: The entire procedure can be finished in 6 hours. Gel-free size selection/purification saves time and prevents handling errors, as well as loss of valuable samples；
2·Complete conversion: The innovative reagent composition converts unmethylated cytosine into uracil at a level greater than 99.9%, with no or negligible inappropriate/error conversion of methylcytosine to thymine (<0.1%) when the indicated range of sample RNA is used；
3.High sensitivity, efficiency and flexibility: Can be used for both non-barcoded (singleplexed) and barcoded (multiplexed) library preparation. Optimized RNA bisulfite method and enhanced adaptor ligation eliminates loss of fragments and selection bias, which enables input RNA to be as low as 5 ng；
4.Extremely convenient: The kit contains all the required components for each step of the RNA library preparation process, which is sufficient for bisulfite conversion, ligation, clean-up, size selection, and library amplification, thereby allowing the bisulfite RNA library preparation to be streamlined for the most reliable and consistent results；
5.Minimal bias: Ultra HiFi amplification enables achievement of reproducibly high yields of bisulfite converted RNA libraries with minimal sequence bias and low error rates。
保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分。
定购信息：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| EpiNext RNA 亚硫酸盐-测序 试剂盒 |
12 次 |
PDF |
A-P-9006 |
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| EpiNext RNA 亚硫酸盐-测序 试剂盒 |
24 次 |
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A-P-9006 |
|
| EpiNext 高保真cDNA 第一链合成试剂盒 |
20 次 |
英文PDF-中文PDF |
A-P-9004 |
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| A&DNext 高保真cDNA第一链合成试剂盒 |
50 次 |
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A-P9006 |
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| 组织总RNA提取试剂盒 |
50 次 |
PDF |
A-RH114-01 |
|
| 组织总RNA提取试剂盒 |
200 次 |
PDF |
A-RH114-02 |
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| 组织/细胞总RNA提取试剂盒II |
50 次 |
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A-RH113-01 |
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| 组织/细胞总RNA提取试剂盒II |
200 次 |
PDF |
A-RH113-02 |
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| 血液总RNA快速提取试剂盒II |
50 次 |
PDF |
A-RH716-01 |
|
| 血液总RNA快速提取试剂盒II |
200 次 |
PDF |
A-RH716-02 |
|
| 多糖多酚植物总RNA快速提取试剂盒（离心柱型） |
50 次 |
PDF |
A-R3202 |
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| 通用植物总RNA大量提取试剂盒（离心柱） |
10 次 |
PDF |
A-R3312 |
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| 血液总RNA提取试剂盒（离心柱型） |
100次 |
PDF |
A-R4002 |
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表观遗传学产品全面解决方案列下：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| DNA羟甲基化定量试剂盒(荧光法) |
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A-P-1037-96 |
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| DNA羟甲基化定量试剂盒(比色法) |
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A-P-1036-48 |
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96 次 |
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| DNA甲基化定量试剂盒(荧光法) |
48 次 |
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A-P-1035-48 |
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| DNA甲基化定量试剂盒(荧光法) |
96 次 |
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A-P-1035-96 |
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| DNA甲基化定量试剂盒(比色法) |
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A-P-1034-48 |
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A-P-1011-96 |
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| 超敏DNA甲基化定量试剂盒 |
48 次 |
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A-P-1021-48 |
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| 超敏DNA甲基化定量试剂盒 |
96 次 |
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A-P-1021-96 |
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| 总DNA甲基化定量试剂盒 |
48 次 |
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A-P-1014-48 |
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| 总DNA甲基化定量试剂盒 |
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关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶，并且区分5-hmC, 5-mC, 和 C，使得研究者能够重新评估他们的DNA甲基化信息，也能够在新样品中寻找DNA羟甲基化。 |
