Pico Methyl-Seq™ Library Prep Kit 货号:A-D5455
背景资料:The Pico Methyl-Seq™ Library Prep Kit provides a streamlined workflow for making WGBS libraries. Input DNA is randomly fragmented during the initial bisulfite treatment step followed by three rounds of amplification with uniquely designed primers. The procedure can accommodate as little as 10 pg input DNA (including that derived from FFPE samples), making it ideal for methylation analysis of precious, limited, and target-enriched samples.
产品描述:Agilent 2200 TapeStation D1K gel of libraries prepared (from B1-G1) using 10 pg, 20 pg, 100 pg, 1 ng, 10 ng, and 100 ng, respectively.
Sample Sources
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The protocol is designed for 10 pg – 100 ng genomic DNA input. DNA should be free of enzymatic inhibitors and can be suspended in water, TE, or a low salt buffer. DNA with low 260/280 or 260/230 ratios should be purified prior to processing using the Genomic DNA Clean & Concentrator™ (Cat. No. D4010) |
Equipment Needed
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Microcentrifuge, thermo-cycler |
Sequencing
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This system is compatible with Illumina’s TruSeq chemistries for the HiSeq™ and MiSeq™ sequencing platforms |
保存建议:请在接收到Pico Methyl-Seq™ Library Prep Kit产品后,按照产品操作说明书对试剂盒内不同组份进行适当的保存。
定购信息:
产品名称 |
规格 |
操作手册 |
货号 |
询价 |
Pico Methyl-Seq™ Library Prep Kit |
10 次 |
PDF |
A-D5455 |
|
Pico Methyl-Seq™ Library Prep Kit |
25 次 |
PDF |
A-D5456 |
|
RRHP™ 5-hmC Library Prep Kit |
12 次 |
PDF |
A-D5450 |
|
RRHP™ 5-hmC Library Prep Kit |
25 次 |
PDF |
A-D5451 |
|
Zymo-Spin™ ChIP Kit |
10 次 |
PDF |
A-D5209 |
|
Zymo-Spin™ ChIP Kit |
25 次 |
PDF |
A-D5210 |
|
Human Matched DNA Set |
1 Set |
PDF |
A-D5018 |
|
Quest 5-hmC™ DNA Enrichment Kit |
50 次 |
PDF |
A-D5421 |
|
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产品名称 |
规格 |
操作手册 |
货号 |
询价 |
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48 次 |
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A-P-1037-48 |
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96 次 |
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A-P-1037-96 |
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A-P-1036-48 |
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A-P-1034-48 |
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A-P-1011-96 |
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48 次 |
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A-P-1021-48 |
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96 次 |
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A-P-1021-96 |
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48 次 |
PDF |
A-P-1014-48 |
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总DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1014-96 |
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5-hmC是近年来在动物组织中发现的,由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能,有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息,也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前,我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
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