ZR DNA-Card Extraction Kit™ 货号：A-D6040
背景资料：DNA can be notoriously difficult to elute from some cellulose-based storage papers (cards). The ZR DNA-Card Extraction Kit™ is designed for simple and rapid purification of inhibitor-free, PCR-quality DNA from blood, saliva, and cells collected onto Guthrie, FTA®, and other storage papers (cards). The procedure is easy and can be completed can be completed in minutes: card punches are added directly to a ZR BashingBead™ Lysis Tube and thoroughly homogenized by bead beating (e.g., Xpedition™ Sample Processor, FastPrep®-24, or similar). Following Proteinase K digestion, the DNA is purified using innovative column technology (see schematic of procedure below). Eluted DNA is ideal for PCR, genotyping, etc.
产品描述：Figure 1. DNA can be effectively purified from blood spotted onto Guthrie and FTA® cards using the ZR DNA-Card Extraction Kit. Briefly, two punches (3.0 mm) from blood spotted onto a Guthrie (C1) and FTA® (D1) card (>1 yr. old) was used for processing with the kit. Eluted DNA was analyzed with a TapeStation (Agilent) shown in the image above. A1 is a molecular weight marker and B1 input DNA from a fresh blood preparation.
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Step 1
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Add card samples (punches) to a ZR BashingBead™ Lysis Tube. Add 400 µl Lysis Solution to the tube.
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Step 2
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Secure lysis tube in a bead beater fitted with a 2 ml tube holder assembly and process at maximum speed.
Note: Processing times may be as little as 40 seconds when using high-speed disrupters (e.g., FastPrep®-24, or similar). See manufacturer’s literature for operating instructions
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Step 3
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Centrifuge the ZR BashingBead™ Lysis Tube for 1 minute |
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Step 4
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To the lysate in the ZR Bashing Bead™ Lysis Tube, add:
2X Digestion Buffer 390 µl
Proteinase K 10 µl
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保存建议：请在接收到ZR DNA-Card Extraction Kit™产品后，按照产品操作说明书对试剂盒内不同组份进行适当的保存。
定购信息：
| 产品名称 |
规格 |
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货号 |
询价 |
| ZR DNA-Card Extraction Kit™ |
50 次 |
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