Zyppy™ Plasmid Maxiprep Kit 货号：A-D4027
背景资料：The Zyppy™ Plasmid Maxiprep Kit employs a modified alkaline lysis method in conjunction with spin-column purification to isolate high quality, endotoxin-free plasmid DNA in minutes. The innovative colored buffers included in the kit permits error-free visualization identification of complete bacterial cell lysis and neutralization. Additionally, the uniquely designed Zymo-Maxi Filter™ column permits lysate clearing without centrifugation while the high capacity DNA-binding Zymo-Spin™ VI column allows for low 2 - 3 ml elution volumes, eliminating the need for DNA precipitation and resuspension steps common to other column-based maxiprep procedures. The purified DNA is suitable for use in transfection, restriction endonuclease digestion, ligation, bacterial transformation, PCR amplification, sequencing, and other sensitive downstream applications.
产品描述：The Zyppy™ Plasmid Maxiprep Kit is designed for use with a centrifuge, a vacuum manifold, or a combination of both procedures and provides the greatest flexibility in large scale plasmid DNA purification from E. coli. An overview of the purification procedure is shown below.
|
Format
|
Spin Column |
|
Processing Time
|
30 min |
|
DNA Purity
|
Plasmid DNA which is well suited for ligation, sequencing, restriction endonuclease digestion, in vitro transcription, and other sensitive applications requiring pure DNA. Typical A(260/280) index is ≥1.8. |
|
Elution Volume
|
≥ 2 ml |
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Plasmid DNA Size
|
Up to 25 kb. |
|
Plasmid DNA Yield
|
≥ 500 µg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. Coli utilized. |
|
Procedure
|
Performed at room temperature (15-30°C) using a centrifuge and/or a vacuum manifold. |
保存建议：请在接收到Zyppy™ Plasmid Maxiprep Kit 产品后，按照产品操作说明书对试剂盒内不同组份进行适当的保存。
定购信息：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| Zyppy™ Plasmid Maxiprep Kit |
10 次 |
PDF |
A-D4027 |
 |
| Zyppy™ Plasmid Maxiprep Kit |
20 次 |
PDF |
A-D4028 |
|
| Pico Methyl-Seq™ Library Prep Kit |
10 次 |
PDF |
A-D5455 |
|
| Pico Methyl-Seq™ Library Prep Kit |
25 次 |
PDF |
A-D5456 |
|
| RRHP™ 5-hmC Library Prep Kit |
12 次 |
PDF |
A-D5450 |
|
| RRHP™ 5-hmC Library Prep Kit |
25 次 |
PDF |
A-D5451 |
|
| Zymo-Spin™ ChIP Kit |
10 次 |
PDF |
A-D5209 |
|
| Zymo-Spin™ ChIP Kit |
25 次 |
PDF |
A-D5210 |
|
| Human Matched DNA Set |
1 Set |
PDF |
A-D5018 |
|
| Quest 5-hmC™ DNA Enrichment Kit |
50 次 |
PDF |
A-D5421 |
|
表观遗传学产品全面解决方案列下：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| DNA羟甲基化定量试剂盒(荧光法) |
48 次 |
PDF |
A-P-1037-48 |
|
| DNA羟甲基化定量试剂盒(荧光法) |
96 次 |
PDF |
A-P-1037-96 |
|
| DNA羟甲基化定量试剂盒(比色法) |
48 次 |
PDF |
A-P-1036-48 |
|
| DNA羟甲基化定量试剂盒(比色法) |
96 次 |
PDF |
A-P-1036-96 |
|
| DNA甲基化定量试剂盒(荧光法) |
48 次 |
PDF |
A-P-1035-48 |
|
| DNA甲基化定量试剂盒(荧光法) |
96 次 |
PDF |
A-P-1035-96 |
|
| DNA甲基化定量试剂盒(比色法) |
48 次 |
PDF |
A-P-1034-48 |
|
| DNA甲基化定量试剂盒(比色法) |
96 次 |
PDF |
A-P-1034-96 |
|
| 通用DNA甲基化试剂盒(定性分析) |
48 次 |
PDF |
A-P-1011-48 |
|
| 通用DNA甲基化试剂盒(定性分析) |
96 次 |
PDF |
A-P-1011-96 |
|
| 超敏DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1021-48 |
|
| 超敏DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1021-96 |
|
| 总DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1014-48 |
|
| 总DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1014-96 |
|
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关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶，并且区分5-hmC, 5-mC, 和 C，使得研究者能够重新评估他们的DNA甲基化信息，也能够在新样品中寻找DNA羟甲基化。 |
