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Epigenetics |
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Epigenetics>【BioLegend】 |
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现货---Universal Methylated Human DNA Standard™ (A-D5011) |
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Universal Methylated Human DNA Standard™
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Format: Used as a control with Zymo Research’s EZ DNA Methylation™, EZ DNA Methylation-Gold™, and EZ DNA Methylation-Direct™ Kits. |
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Control DNA: 250 ng/µl in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0) |
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Primers: Two control primers are supplied at a concentration of 20 µM in 20 µl TE. |
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1 set can be used for up to 20 separate bisulfite treatments with either the EZ DNA Methylation™ Kit or the EZ DNA Methylation-Gold™ Kit. |
Descriptions
The Universal Methylated Human DNA Standard includes enzymatically methylated DNA together with a specially-designed primer set to be used in conjunction with Zymo Research’s EZ DNA Methylation™, EZ DNA Methylation-Gold™, and EZ DNA Methylation-Direct™ Kits to assess the efficiency of bisulfite-mediated conversion of DNA. The DNA was isolated from a human male liver and modified with SssI methyltransferase (EC 2.1.1.37). The DNA is methylated at cytosine positions comprising CG dinucleotides. The primer set herein has been designed to amplify a fragment of the supplied DNA following bisulfite treatment. The methylated cytosines comprising CG dinucleotides remain unconverted following bisulfite treatment, whereas nonmethylated cytosines are converted into uracil and detected as thymine following PCR.
Universal Methylated Human DNA Standard, 5 µg/20 µl. Source: DNA derived from male human liver. DNA has methylated cytosines (C5) at all CG dinucleotide positions.
Product |
Catalog No. |
Size |
Price(RMB) |
Protocol |
Universal Methylated Human DNA Standard™ |
D5011 |
1 set(20次) |
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表观遗传学产品全面解决方案列下:
产品名称 |
规格 |
操作手册 |
货号 |
询价 |
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英文PDF--中文PDF |
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英文PDF--中文PDF |
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英文PDF--中文PDF |
A-P-1037-48 |
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96 次 |
英文PDF--中文PDF |
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48 次 |
英文PDF--中文PDF |
A-P-1035-48 |
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96 次 |
英文PDF--中文PDF |
A-P-1035-96 |
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48 次 |
英文PDF--中文PDF |
A-P-1034-48 |
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96 次 |
英文PDF--中文PDF |
A-P-1034-96 |
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通用DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1011-48 |
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通用DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1011-96 |
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超敏DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1021-48 |
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超敏DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1021-96 |
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48 次 |
英文PDF--中文PDF |
A-P-1014B-48 |
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总DNA甲基化定量试剂盒 |
96 次 |
英文PDF--中文PDF |
A-P-1014B-96 |
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