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Epigenetics |
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| Epigenetics>【BioLegend】 |
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Bisulfite Converted Universal Methylated Human DNA Standard( |
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Bisulfite Converted Universal Methylated Human DNA Standard
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Purified male human DNA that has been methylated at all cytosine positions within the CG dinucleotide context by M.SssI methyltransferase and treated with sodium bisulfite for direct downstream methylation applications. |
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Control primer pair is designed to amplify a fragment of the human MLH1 mismatch repair gene following bisulfite conversion. |
The Bisulfite Converted Universal Human DNA Standard contains bisulfite-treated DNA derived from the Universal Methylated Human DNA Standard. The supplied DNA has been methylated at all cytosine positions within the CG dinucleotide context using M.SssI methyltransferase (EC 2.1.1.37). Following methylation, the DNA was treated with sodium bisulfite according to the protocol described in our EZ DNA Methylation-Direct™ Kit. In this kit the methylated cytosines are unmodified by sodium bisulfite, whereas all non-methylated cytosines are converted to uracil, which are detected as thymine in subsequent PCR.
The included primer set is designed to amplify a fragment of the human MLH1 mismatch repair gene (GenBank Accession# U83845, nucleotides 804 through 986) and can be used directly with the Bisulfite Converted Universal Methylated Human DNA Standard.
| Product |
Catalog No. |
Size |
Price(RMB) |
Protocol |
| Bisulfite Converted Universal Methylated Human DNA Standard |
A-D5015 |
1 set(50次) |
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PDF
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表观遗传学产品全面解决方案列下：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| DNA羟甲基化定量试剂盒(比色法) |
48 次 |
英文PDF--中文PDF |
A-P-1036-48 |
 |
| DNA羟甲基化定量试剂盒(比色法) |
96 次 |
英文PDF--中文PDF |
A-P-1036-96 |
|
| DNA羟甲基化定量试剂盒(荧光法) |
48 次 |
英文PDF--中文PDF |
A-P-1037-48 |
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| DNA羟甲基化定量试剂盒(荧光法) |
96 次 |
英文PDF--中文PDF |
A-P-1037-96 |
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| DNA甲基化定量试剂盒(荧光法) |
48 次 |
英文PDF--中文PDF |
A-P-1035-48 |
|
| DNA甲基化定量试剂盒(荧光法) |
96 次 |
英文PDF--中文PDF |
A-P-1035-96 |
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| DNA甲基化定量试剂盒(比色法) |
48 次 |
英文PDF--中文PDF |
A-P-1034-48 |
|
| DNA甲基化定量试剂盒(比色法) |
96 次 |
英文PDF--中文PDF |
A-P-1034-96 |
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| 通用DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1011-48 |
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| 通用DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1011-96 |
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| 超敏DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1021-48 |
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| 超敏DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1021-96 |
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| 总DNA甲基化定量试剂盒 |
48 次 |
英文PDF--中文PDF |
A-P-1014B-48 |
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| 总DNA甲基化定量试剂盒 |
96 次 |
英文PDF--中文PDF |
A-P-1014B-96 |
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推荐阅读：
关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶，并且区分5-hmC, 5-mC, 和 C，使得研究者能够重新评估他们的DNA甲基化信息，也能够在新样品中寻找DNA羟甲基化。 |
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