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Epigenetics |
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| Epigenetics>【SBI】 |
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EZ-96 DNA Methylation™ Kit (A-D5003) |
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EZ-96 DNA Methylation™ Kit (Shallow Well Format)
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High throughput (96-well), proven procedure for bisulfite conversion of DNA. |
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96-well desulphonation and recovery of bisulfite-treated DNA. |
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Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc. |
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Format: Chemical denaturation of DNA followed by sodium bisulfite modification, Spin Plate. |
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Recovery Volume: Purified, bisulfite treated DNA is recovered in ≥30 µl provided elution buffer. |
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Input DNA: 500 pg - 2 µg DNA per treatment with 200 - 500 ng being optimal. |
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Sample Sources: Plasmid, genomic, and endonuclease digested DNAs. |
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Equipment Needed: Centrifuge with microplate carriers |
Descriptions
The EZ-96 DNA Methylation™ Kit features a high-throughput (96-well), simplified procedure that streamlines bisulfite conversion of DNA. The kit is based on the three-step reaction that takes place between cytosine and sodium bisulfite where cytosine is converted into uracil. The product’s innovative desulphonation technology eliminates otherwise cumbersome precipitations. The kit is designed to reduce template degradation, minimize DNA loss during treatment and clean-up, while ensuring complete conversion of the DNA. Purified, converted DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc. An outline comparing the EZ DNA Methylation™ Kit procedure to Zymo Research’s other methylation kits is shown to the right.
| Product |
Catalog No. |
Size |
Price(RMB) |
Protocol |
EZ-96 DNA Methylation™ Kit
(shallow well) |
A-D5003 |
2x96 Rxns. |
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PDF
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关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶，并且区分5-hmC, 5-mC, 和 C，使得研究者能够重新评估他们的DNA甲基化信息，也能够在新样品中寻找DNA羟甲基化。
表观遗传学产品全面解决方案列下：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| DNA羟甲基化定量试剂盒(比色法) |
48 次 |
PDF |
A-P-1036-48 |
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| DNA羟甲基化定量试剂盒(比色法) |
96 次 |
PDF |
A-P-1036-96 |
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| DNA甲基化定量试剂盒(荧光法) |
48 次 |
PDF |
A-P-1035-48 |
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| DNA甲基化定量试剂盒(荧光法) |
96 次 |
PDF |
A-P-1035-96 |
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| DNA甲基化定量试剂盒(比色法) |
48 次 |
PDF |
A-P-1034-48 |
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| DNA甲基化定量试剂盒(比色法) |
96 次 |
PDF |
A-P-1034-96 |
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| 通用DNA甲基化试剂盒(定性分析) |
48 次 |
PDF |
A-P-1011-48 |
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| 通用DNA甲基化试剂盒(定性分析) |
96 次 |
PDF |
A-P-1011-96 |
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| 超敏DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1021-48 |
|
| 超敏DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1021-96 |
|
| 总DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1014-48 |
|
| 总DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1014-96 |
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