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Epigenetics |
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| Epigenetics>【SBI】 |
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EZ-96 DNA Methylation™ Kit (A-D5004) |
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EZ-96 DNA Methylation™ Kit (Deep Well Format)
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High throughput (96-well), proven procedure for bisulfite conversion of DNA. |
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96-well desulphonation and recovery of bisulfite-treated DNA. |
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Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc. |
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Format: Chemical denaturation of DNA followed by sodium bisulfite modification, Spin Plate. |
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Recovery Volume: Purified, bisulfite treated DNA is recovered in ≥15 µl provided elution buffer. |
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Input DNA: 500 pg - 2 µg DNA per treatment with 200 - 500 ng being optimal. |
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Sample Sources: Plasmid, genomic, and endonuclease digested DNAs. |
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Equipment Needed: Centrifuge with microplate carriers. |
Descriptions
The EZ-96 DNA Methylation™ Kit features a high-throughput (96-well), simplified procedure that streamlines bisulfite conversion of DNA. The kit is based on the three-step reaction that takes place between cytosine and sodium bisulfite where cytosine is converted into uracil. The product’s innovative desulphonation technology eliminates otherwise cumbersome precipitations. The kit is designed to reduce template degradation, minimize DNA loss during treatment and clean-up, while ensuring complete conversion of the DNA. Purified, converted DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc. An outline comparing the EZ DNA Methylation™ Kit procedure to Zymo Research’s other methylation kits is shown to the right.
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