The EZ DNA Methylation-Gold™ Kit is a refinement of our popular EZ DNA Methylation™ Kit. The EZ DNA Methylation-Gold™ Kit integrates the DNA denaturation and bisulfite conversion processes into one step. This is accomplished using temperature denaturation to replace chemical denaturation with sodium hydroxide in the previous protocol. Also, the kit has been streamlined for high yield recovery of DNA following bisulfite treatment. Both kits are based on a three step reaction process between cytosine and sodium bisulfite resulting in cytosine being converted into uracil. The EZ DNA Methylation-Gold™ and EZ DNA Methylation™ Kits share innovative in-column desulphonation technology that eliminates cumbersome DNA precipitation steps while providing researchers consistent results every time. The kits have been designed to minimize template degradation, loss of DNA during treatment and clean-up, and to provide complete conversion of unmethylated cytosines. Recovered DNA is ideal for PCR amplification for downstream analyses including restriction endonuclease digestion, sequencing, microarrays, etc. The entire procedure can be completed in less than 3 hours.