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Epigenetics |
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| Epigenetics>【SBI】 |
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EZ-96 DNA Methylation-Gold™ Kit (A-D5007) |
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EZ-96 DNA Methylation-Gold™ Kit (Shallow Well Format)
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Complete bisulfite conversion of GC-rich DNA in less than 3 hours.
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A coupled DNA denaturation/bisulfite treatment step streamlines the conversion of unmethylated cytosines into uracil.
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DNA precipitations are omitted. Instead, DNA is cleaned and desulphonated in a single step.
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Eluted, ultra-pure DNA is ideal for use in subsequent molecular-based analyses.
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Format: Rapid heat denaturation of DNA followed by sodium bisulfite modification, Spin Plate. |
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Recovery Volume: Purified, bisulfite treated DNA is recovered in ≥30 µl provided elution buffer. |
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Input DNA: 500 pg - 2 µg DNA per treatment with 200 - 500 ng being optimal. |
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Sample Sources: Plasmid, genomic, and endonuclease digested DNAs. |
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Equipment Needed: Centrifuge with microplate carriers, thermalcycler. |
Descriptions
The 96-well format EZ-96 DNA Methylation-Gold™ Kit is a refinement of our popular EZ-96 DNA Methylation™Kit. The EZ-96 DNA Methylation-Gold™ Kit integrates the DNA denaturation and bisulfite conversion processes into one-step. This is accomplished using temperature denaturation to replace chemical denaturation with sodium hydroxide in the previous protocol. Also, the kit has been streamlined for high yield recovery of DNA following DNA bisulfite conversion. Both kits are based on a three-step reaction process between cytosine and sodium bisulfite resulting in cytosine being converted into uracil. The EZ-96 DNA Methylation-Gold™ and EZ-96 DNA Methylation™ Kits share innovative in-column desulphonation technology that eliminates cumbersome DNA precipitation steps while providing researchers consistent results every time. The kits have been designed to minimize template degradation, loss of DNA during treatment and clean-up, and to provide complete conversion of unmethylated cytosines. Recovered DNA is ideal for PCR amplification for downstream analyses including restriction endonuclease digestion, sequencing, microarrays, etc.
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Catalog No. |
Size |
Price(RMB) |
Protocol |
EZ-96 DNA Methylation-Gold™ Kit
(shallow well) |
A-D5007 |
2x96 Rxns. |
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