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Epigenetics |
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| Epigenetics>【SBI】 |
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EZ-96 DNA Methylation-Direct™ Kit (A-D5023) |
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EZ-96 DNA Methylation-Direct™ Kit (Deep Well Format)
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High throughput (96-well), complete bisulfite conversion of DNA directly* from blood, tissue, or cells. |
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Compatible with small sample inputs – as few as 10 cells or 50 pg DNA. |
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Well-suited for FFPE and LCM-derived samples. |
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Cells: Compatible with cells from solid tissue, tissue culture, whole blood, buffy coat, biopsies, LCM (Laser-Capture Micro-Dissection) and FFPE samples, etc. The number of cells per standard treatment can range from 10 - 105 cells. For optimal results, the cell number should be from 1 x 103 - 8 x 104 cells. |
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Purified DNA: Samples containing 50 pg - 2 µg of DNA. For optimal results, the amount of input DNA should be from 200 to 500 ng. |
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Conversion Efficiency: > 99.5% of non-methylated C residues are converted to U; > 99.5% protection of methylated cytosines. |
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DNA Recovery: > 80% |
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Sensitivity of Detection (Lower Limit): 10 cells for successful PCR amplification. |
Descriptions
The EZ DNA Methylation-Direct™ Kit is a further refinement of our popular EZ DNA Methylation™ and EZ DNA Methylation-Gold™ Kits. The EZ DNA Methylation-Direct™ Kit features simple and reliable DNA bisulfite conversion directly from blood, tissue, and cells without the prerequisite for DNA purification. The increased sensitivity of this kit makes it possible to amplify bisulfite converted DNA from as few as 10 cells or 50 pg DNA. Like the EZ DNA Methylation-Gold™ Kit, DNA denaturation and bisulfite conversion processes are combined into a single step (see below). All kits streamline the three step process of bisulfite conversion of non-methylated cytosine in DNA into uracil. In addition the methylation kits share innovative in-column desulphonation technology that eliminates otherwise cumbersome DNA precipitation steps while ensuring researchers consistent results every time. The kits have been designed to minimize template degradation, loss of DNA during treatment and clean-up, and to provide complete conversion of unmethylated cytosines. Recovered DNA is ideal for PCR amplification for downstream analyses including restriction endonuclease digestion, sequencing, microarrays, etc.
An outline comparing the EZ DNA Methylation-Direct™ Kit procedure to Zymo Research’s other methylation kits is shown below.
| Product |
Catalog No. |
Size |
Price(RMB) |
Protocol |
EZ-96 DNA Methylation-Direct™ Kit
(deep-well) |
A-D5023 |
2x96 Rxns. |
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PDF |
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表观遗传学产品全面解决方案列下：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| DNA羟甲基化定量试剂盒(比色法) |
48 次 |
PDF |
A-P-1036-48 |
 |
| DNA羟甲基化定量试剂盒(比色法) |
96 次 |
PDF |
A-P-1036-96 |
|
| DNA甲基化定量试剂盒(荧光法) |
48 次 |
PDF |
A-P-1035-48 |
|
| DNA甲基化定量试剂盒(荧光法) |
96 次 |
PDF |
A-P-1035-96 |
|
| DNA甲基化定量试剂盒(比色法) |
48 次 |
PDF |
A-P-1034-48 |
|
| DNA甲基化定量试剂盒(比色法) |
96 次 |
PDF |
A-P-1034-96 |
|
| 通用DNA甲基化试剂盒(定性分析) |
48 次 |
PDF |
A-P-1011-48 |
|
| 通用DNA甲基化试剂盒(定性分析) |
96 次 |
PDF |
A-P-1011-96 |
|
| 超敏DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1021-48 |
|
| 超敏DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1021-96 |
|
| 总DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1014-48 |
|
| 总DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1014-96 |
|
推荐阅读：
关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶，并且区分5-hmC, 5-mC, 和 C，使得研究者能够重新评估他们的DNA甲基化信息，也能够在新样品中寻找DNA羟甲基化。 |
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