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| DNA>常用PCR相关/DNA Clean-up |
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A&D DNA Clean & Concentrator™-5 （D4003 D4013） |
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Simple, rapid recovery of ultra-pure DNA from PCR, endonuclease digestions, “crude” DNA preps., etc. Perfect for post-RT cDNA clean-up and purification of sequencing-ready DNA from M13 phage infected culture supernatants. |
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Column and deep-well filtration plate designs allow DNA to be eluted at high concentrations into minimal volumes of water or TE buffer. |
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Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, etc. |
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Format: Spin Column (capped/uncapped) |
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Sample Sources: DNA from PCR, restriction endonuclease digestions, “crude” DNA preparations, etc. |
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DNA Size Limits: 50 bp to 23 kb |
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DNA Recovery: Typically, ≤5 µg total DNA can be eluted with ≥6 µl water or low salt elution buffer (≥10 µl for ZR-96 DCC™-5). For DNA 50 bp to 10 kb the recovery is 70-95%. For DNA 11 kb to 23 kb the recovery is 50-70%. |
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DNA Purity: Highly purified DNA is eluted with water and is especially well suited for sequencing, ligation reactions, and restriction endonuclease digestions. |
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Detergent Tolerance: ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS |
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Equipment Needed: Microcentrifuge |
Descriptions
The DNA Clean & Concentrator™-5 (DCC™-5) and ZR-96 DNA Clean & Concentrator™-5 products provide hassle-free, rapid purification and concentration of up to 5 µg (per sample) of high quality DNA from PCR, endonuclease digestions, or "crude" DNA preparations. Both can also be used for post-RT cDNA clean-up and purification of sequencing-ready DNA from M13 phage. The DNA Clean & Concentrator™ procedure is easy: Simply add the specially formulated DNA Binding Buffer to your samples and transfer the mixtures to the supplied Zymo-Spin™ Column or Zymo-Spin™ Filtration Plate, then spin, wash, and elute the DNA. There is no need for organic denaturants or chloroform. Instead, the products feature unique silica-based matrix technologies to yield high quality, purified DNA that is especially well suited for DNA sequencing, microarray analysis, PCR, nucleotide blotting, restriction endonuclease digestion, etc. The entire DNA purification/concentration procedure can be performed in just minutes.
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Catalog No. |
Size |
Price |
Store |
DNA Clean & Concentrator™-5
(uncapped columns) |
D4003 |
50 Preps. |
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D4004 |
200 Preps. |
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DNA Clean & Concentrator™-5
(capped columns) |
D4013 |
50 Preps. |
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D4014 |
200 Preps. |
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Sample
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D4003S |
5 Preps. |
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