|
DNA |
|
|
|
|
|
|
DNA>Plasmid/Gel Recovery/M13 |
|
|
|
|
Zymoclean™ Gel DNA Recovery Kit (D4001 D4002) |
|
|
|
• |
Quick (15 minute) recovery of ultra-pure DNA from agarose gels. |
• |
Column design allows DNA to be eluted at high concentrations into minimal volumes of solvent. |
• |
Eluted DNA is well suited for use in DNA ligation reactions, sequencing, DNA labeling reactions, PCR, etc. |
• |
Omits the use of glass beads, organic denaturants, and proteinases. |
• |
Format: Spin Column |
• |
Sample Sources: DNA excised from TAE/TBE-buffered agarose gels. |
• |
DNA Size Limits: 75 bp to 23 kb |
• |
DNA Recovery: Typically, up to 5 µg total DNA per column can be eluted with ≥6 µl water. For DNA 75 bp to 10 kb the recovery is 70-90%. For DNA 11 kb to 23 kb the recovery is 50-70%. |
• |
DNA Purity: High quality, purified DNA is eluted with water which is especially well suited for sequencing, ligation reactions, andrestriction endonuclease digestions. |
• |
Detergent Tolerance: ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS |
• |
Equipment Needed: Microcentrifuge |
Descriptions
The Zymoclean™ Gel DNA Recovery Kit and ZR-96 Zymoclean™ Gel DNA Recovery Kit provide simple, rapid methods for the purification and concentration of high quality DNA from agarose gel slices. Both products feature a single buffer system that allows for efficient dissolving of agarose gel slices and the subsequent adsorption of DNA onto the matrices of the supplied Zymo-Spin™ I Columns or Zymo-Spin™ I-96 Filtration Plate. The DNA is washed twice then eluted with a small volume of water. The entire DNA purification/concentration procedure typically takes about 15-20 minutes. The procedure is easy: simply add the specially formulated Agarose Dissolving Buffer (ADB™) to the gel slice containing your DNA sample, let dissolve, and transfer the mixture to the supplied column or filtration plate, then spin, wash, and elute the DNA. There is no need for organic denaturants or chloroform. Instead, the products utilize unique column and filtration plate technologies to yield high quality, purified DNA in just minutes. DNA purified using the Zymoclean™ Gel DNA Recovery Kit and ZR-96 Zymoclean™ Gel DNA Recovery Kit is perfectly suited for use in DNA ligation reactions, sequencing, DNA labeling reactions, PCR, etc.
Product |
Catalog No. |
Size |
Price(RMB) |
Prococol |
Zymoclean™ Gel DNA Recovery Kit
(uncapped columns) |
D4001 |
50 Preps. |
|
|
|
D4002 |
200 Preps. |
|
|
Zymoclean™ Gel DNA Recovery Kit
(capped columns) |
D4007 |
50 Preps. |
|
|
|
D4008 |
200 Preps. |
|
|
Free Sample
|
D4001S |
10 Preps. |
|
|
|
|
|
|
|
|