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RNA |
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| RNA>PAGE/Gel RNA Recovery |
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Zymoclean™ Gel RNA Recovery Kit (A-R1011) |
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Zymoclean™ Gel RNA Recovery Kit 货号：A-R1011
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Quick (30 minute) recovery of purified RNA fragments from agarose gels. |
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Fast-Spin column technology allows RNA to be eluted into minimal volumes (≥6 µl). |
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Eluted RNA is ultra clean and ready for subsequent analysis and molecular manipulation. |
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Format: Spin Column |
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Sample Sources: Single- or double-stranded RNA fragments (≥200 nucleotides) resolved in MOPS, TAE and TBE buffered agarose gels. |
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RNA Recovery: The recovery rate for fragments ≥500 nucleotides is ≥80 %. Total binding capacity of the supplied Zymo-Spin IC™ Columns is ≥5 µg. |
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RNA Purity: High quality RNA (A260/A280 > 1.8, A260/A230 > 1.8) suitable for all downstream RNA-based manipulations. |
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RNA Storage: RNA is eluted with RNase-free water and can be stored at ≤ -70°C. The addition of RNase inhibitors is optional but highly recommended for prolonged storage. |
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Equipment Needed: Microcentrifuge, 37 to 65ºC heat source. |
Descriptions
Zymoclean™ Gel RNA Recovery Kit provides a quick and efficient purification method for recovery of RNA fragments from agarose gels. The procedure combines a unique, single-step agarose dissolving/RNA binding buffer with Fast-Spin column technology to yield high quality, purified RNA in just minutes. The purified RNA eluted with RNase-free water into small volumes is highly concentrated and suitable for subsequent RNA-based manipulations including RT-PCR.
| Product |
Catalog No. |
Size |
Price(RMB) |
Protocol |
| Zymoclean™ Gel RNA Recovery Kit |
A-R1011 |
50 Preps. |
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Sample
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A-R1011S |
5 Preps. |
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表观遗传学产品全面解决方案列下：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| DNA羟甲基化定量试剂盒(荧光法) |
48 次 |
PDF |
A-P-1037-48 |
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| DNA羟甲基化定量试剂盒(荧光法) |
96 次 |
PDF |
A-P-1037-96 |
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| DNA羟甲基化定量试剂盒(比色法) |
48 次 |
PDF |
A-P-1036-48 |
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| DNA羟甲基化定量试剂盒(比色法) |
96 次 |
PDF |
A-P-1036-96 |
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| DNA甲基化定量试剂盒(荧光法) |
48 次 |
PDF |
A-P-1035-48 |
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| DNA甲基化定量试剂盒(荧光法) |
96 次 |
PDF |
A-P-1035-96 |
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| DNA甲基化定量试剂盒(比色法) |
48 次 |
PDF |
A-P-1034-48 |
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| DNA甲基化定量试剂盒(比色法) |
96 次 |
PDF |
A-P-1034-96 |
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| 通用DNA甲基化试剂盒(定性分析) |
48 次 |
PDF |
A-P-1011-48 |
|
| 通用DNA甲基化试剂盒(定性分析) |
96 次 |
PDF |
A-P-1011-96 |
|
| 超敏DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1021-48 |
|
| 超敏DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1021-96 |
|
| 总DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1014-48 |
|
| 总DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1014-96 |
|
推荐阅读：
关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶，并且区分5-hmC, 5-mC, 和 C，使得研究者能够重新评估他们的DNA甲基化信息，也能够在新样品中寻找DNA羟甲基化。
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