RNA Clean & Concentrator™-25 provides a simple and reliable method for the rapid preparation of up to ~25 µg (per prep.) of high-quality RT-PCR-ready RNA. This simple procedure is based on the use of a unique single-buffer system and Fast-Spin column technology.
The procedure is easy: just add the buffer to your sample, adjust the conditions for binding by adding ethanol, and the cleaned RNA is then concentrated into ≥25 µl of RNase-free water using a Zymo-Spin™ IIC Column. RNA fragments (≥17 bases) can be safely treated and recovered using this kit. The result is highly-concentrated, purified RNA that is suitable for subsequent RNA-based methods including RT-PCR, hybridization, etc.
The entire procedure typically takes about 5 minutes.