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RNA |
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| RNA>RNA Clean-up |
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RNA Clean & Concentrator™-25 (A-R1017 A-R1018) |
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Quick (5 minute) method for cleaning and concentrating RNA. |
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Fast-Spin column technology allows RNA to be eluted into minimal volumes (≥25 µl). |
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Eluted RNA is ultra clean and ready for subsequent analysis and molecular manipulation. |
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Format: Spin Column |
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Sample Sources: RNA fragments (≥17 nucleotides): DNase treated RNA, labeled RNA fragments, in vitro transcription products, etc. |
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RNA Recovery: Typically, RNA is eluted into as little as 25 µl RNase-free water allowing for a highly concentrated sample. The RNA binding capacity of the supplied Zymo-Spin™ IIC Columns is ~25 µg. |
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RNA Purity: High quality RNA (A260/A280 >1.8, A260/A230 >1.8) suitable for all downstream RNA-based manipulations. |
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RNA Storage: RNA is eluted with RNase-free water and can be stored at ≤ -70°C. The addition of RNase inhibitors is optional but highly recommended for prolonged storage. |
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Equipment Needed: microcentrifuge |
Descriptions
RNA Clean & Concentrator™-25 provides a simple and reliable method for the rapid preparation of up to ~25 µg (per prep.) of high-quality RT-PCR-ready RNA. This simple procedure is based on the use of a unique single-buffer system and Fast-Spin column technology.
The procedure is easy: just add the buffer to your sample, adjust the conditions for binding by adding ethanol, and the cleaned RNA is then concentrated into ≥25 µl of RNase-free water using a Zymo-Spin™ IIC Column. RNA fragments (≥17 bases) can be safely treated and recovered using this kit. The result is highly-concentrated, purified RNA that is suitable for subsequent RNA-based methods including RT-PCR, hybridization, etc.
The entire procedure typically takes about 5 minutes.
| Product |
Catalog No. |
Size |
Price(RMB) |
Protocol |
| RNA Clean & Concentrator™-25 |
A-R1017 |
50 Preps. |
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A-R1018 |
100 Preps. |
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