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RNA |
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| RNA>Cells&Soft Tissue |
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Quick-RNA™ MicroPrep (R1050) |
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10 minute method for isolating RNA (up to 5 μg) from a wide range of cell types and tissue samples. |
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Fast-Spin column technology allows ultra clean RNA to be eluted into minimal volumes (≥6 μl). |
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Omits the use of organic denaturants and proteases. |
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Formats: Spin column. |
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Sample Sources: Cells from culture or tissue samples, buccal cells, buffy coat, plasma, serum, and other biological liquids. Not compatible with whole blood. (For purification of RNA from whole blood, see the ZR Whole-Blood RNA MiniPrep (R1020)) |
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Sample Size: 1 to 105 cells (≤100 μl plasma, serum). |
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RNA Recovery: Up to 5 μg RNA can be eluted into ≥6 μl RNase-free water allowing for a highly concentrated sample. |
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RNA Purity: High quality RNA (A260/A280 > 1.8, A260/A230 > 1.8) suitable for all downstream RNA-based manipulations. |
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RNA Storage: 70ºC |
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Equipment: Microcentrifuge. |
The Quick-RNA™ MicroPrep is an innovative product designed for the easy, reliable, and rapid isolation of up to 5 μg total RNA from cultured cells or tissue samples. The procedure combines a unique, single-step RNA extraction/binding buffer with Fast-Spin column technology to yield high quality RNA in about 10 minutes. The Quick-RNA™ MicroPrep allows for the efficient recovery of total RNA from 1 to 105 cells or tissue (up to ~10 mg). The method is easy: simply add the provided ZR RNA Buffer to extract total RNA from the cells of interest then purify the RNA using the provided Zymo-Spin™ IC Column. The result is highly-concentrated, purified RNA that is suitable for subsequent RNA-based methods including RT-PCR, hybridization, etc.
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PCR amplification of β-actin transcript (353 bp fragment shown) following RNA isolation from human epithelial cells (HCT 116) with the Quick-RNA™ MicroPrep: RT-PCR (lane 1, 2), PCR negative control (RNA template; lane 3, 4).
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| Product |
Catalog No. |
Size |
Price
(RMB)
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Protocol |
| Quick-RNA™ MicroPrep |
R1050 |
50 Preps. |
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