 |
RNA |
 |
|
|
|
|
|
 |
| RNA>Solid&FFPE Tissue |
| |
|
| |
| |
Pinpoint™ Slide RNA Isolation System I (A-R1003) |
| |
|
|
| • |
Allows for the isolation of total RNA from tissue mounted on glass slides. |
| • |
Simple procedure combines Pinpoint™ tissue sampling technology with a onestep RNA extraction/purification method. |
| • |
Isolates RNA that is suitable for use in RNA-based procedures including RT-PCR. |
| • |
Omits the use of organic denaturants as well as proteinases. |
| • |
Format: Pinpoint™ Sampling, Spin Column. |
| • |
Recovery: Typically, up to 5 µg RNA is eluted into ≥6 µl elution buffer allowing for a highly concentrated sample. Recovery is approximately 80-95% of input RNA. |
| • |
RNA Storage: Recommended that 1 U/10 µl RNase inhibitor be added to the RNA prior to storage at -70°C. |
| • |
RNA Size Limits: From 200 bases up to 35 kb. |
| • |
Sample Sources: Cells from tissue sections on glass slides. |
Descriptions
The Pinpoint™ Slide RNA Isolation System I is an innovative product designed by those at Zymo Research to isolate RNA from any targeted area of tissue on a microscopic slide. The system combines a powerful Pinpoint™ tissue sampling method with a unique, single-step RNA extraction/binding buffer that includes Fast-Spin column technology to yield high quality purified RNA. This product makes targeted tissue RNA isolation simple and quick. There is no use of organic solvents or other toxic reagents used in this method. There is also no need for expensive specialized equipment or computer software. This kit allows for the efficient recovery of RNA from fresh tissue sections for subsequent RNA analyses including RT-PCR.
| Product |
Catalog No. |
Size |
Price(RMB) |
Protocol |
| Pinpoint™ Slide RNA Isolation System I |
A-R1003 |
50 Preps. |
 |
|
| |
表观遗传学产品全面解决方案列下：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| DNA羟甲基化定量试剂盒(荧光法) |
48 次 |
PDF |
A-P-1037-48 |
 |
| DNA羟甲基化定量试剂盒(荧光法) |
96 次 |
PDF |
A-P-1037-96 |
|
| DNA羟甲基化定量试剂盒(比色法) |
48 次 |
PDF |
A-P-1036-48 |
|
| DNA羟甲基化定量试剂盒(比色法) |
96 次 |
PDF |
A-P-1036-96 |
|
| DNA甲基化定量试剂盒(荧光法) |
48 次 |
PDF |
A-P-1035-48 |
|
| DNA甲基化定量试剂盒(荧光法) |
96 次 |
PDF |
A-P-1035-96 |
|
| DNA甲基化定量试剂盒(比色法) |
48 次 |
PDF |
A-P-1034-48 |
|
| DNA甲基化定量试剂盒(比色法) |
96 次 |
PDF |
A-P-1034-96 |
|
| 通用DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1011-48 |
|
| 通用DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1011-96 |
|
| 超敏DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1021-48 |
|
| 超敏DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1021-96 |
|
| 总DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1014-48 |
|
| 总DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1014-96 |
|
推荐阅读：
关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶，并且区分5-hmC, 5-mC, 和 C，使得研究者能够重新评估他们的DNA甲基化信息，也能够在新样品中寻找DNA羟甲基化。
|
| |
|
|
| |
|
