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  Zymoclean™ Large Fragment DNA Recovery Kit (D4045)
   
Zymoclean™ Large Fragment DNA Recovery Kit
 

Quick (15 minute) spin column recovery of large-sized DNA (e.g., genomic, plasmid (BAC/PAC),
viral, phage, etc.) from agarose gels. No messy precipitations!
Unique column design for low volume (≥10 μl) elution of ultra-pure, high-yield DNA.
Eluted DNA is well suited for use in PCR, sequencing, endonuclease digestion, ligation, etc.

 
 

Format: Spin Column
Sample Sources: DNA excised from TAE and TBE-buffered agarose gels.
DNA Size Limits: 50 bp to ~200 kb
DNA Recovery: Typically, up to 10 μg total DNA per column can be eluted into ≥10 μl water. For DNA 50 bp to 23 kb the recovery is 70-90%. For DNA ≥23 kb, the recovery is 50-70%.
DNA Purity: High-quality, purified DNA is eluted with water making it especially well suited for sequencing and ligation reactions.
Detergent Tolerance: ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS
Equipment Needed: Microcentrifuge

 

 

Descriptions

The Zymoclean™ Large Fragment DNA Recovery Kit provides a streamlined method for the rapid purification and concentration of high-quality large-sized DNA from agarose gels. Simply add the specially formulated Agarose Dissolving Buffer (ADB) to the gel slice containing a DNA sample, let dissolve, and then transfer to the supplied Zymo-Spin™ IC-XL Column. There is no need for organic denaturants or chloroform. Instead, the product utilizes unique spin column technology to yield high-quality, purified DNA in just minutes. DNA purified using the Zymoclean™ Large Fragment DNA Recovery Kit is ideal for PCR, sequencing, endonuclease digestion, ligation, etc. The entire procedure typically takes about 15 minutes.

 

 

DNA recovery (lanes 1-6) from HindIII-digested lambda DNA (lane C) and lambda DNA (λ) using the Zymoclean™ Large Fragment DNA Recovery Kit. Lanes 1, 3: 23 kb and 2, 4: 9 kb bands, respectively. Lanes 5, 6: ~48 kb bands.

 
 

Product Catalog No. Size Price(Price) Protocol
Zymoclean™ Large Fragment DNA Recovery Kit D4045 25 Preps. 点击这里给我发消息
  D4046 100 Preps.
 
 

 

 
 
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