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DNA |
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DNA>Plasmid/Gel Recovery/M13 |
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Zymoclean™ Large Fragment DNA Recovery Kit (D4045) |
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Zymoclean™ Large Fragment DNA Recovery Kit
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Quick (15 minute) spin column recovery of large-sized DNA (e.g., genomic, plasmid (BAC/PAC),
viral, phage, etc.) from agarose gels. No messy precipitations! |
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Unique column design for low volume (≥10 μl) elution of ultra-pure, high-yield DNA. |
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Eluted DNA is well suited for use in PCR, sequencing, endonuclease digestion, ligation, etc. |
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Format: Spin Column |
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Sample Sources: DNA excised from TAE and TBE-buffered agarose gels. |
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DNA Size Limits: 50 bp to ~200 kb |
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DNA Recovery: Typically, up to 10 μg total DNA per column can be eluted into ≥10 μl water. For DNA 50 bp to 23 kb the recovery is 70-90%. For DNA ≥23 kb, the recovery is 50-70%. |
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DNA Purity: High-quality, purified DNA is eluted with water making it especially well suited for sequencing and ligation reactions. |
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Detergent Tolerance: ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS |
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Equipment Needed: Microcentrifuge |
Descriptions
The Zymoclean™ Large Fragment DNA Recovery Kit provides a streamlined method for the rapid purification and concentration of high-quality large-sized DNA from agarose gels. Simply add the specially formulated Agarose Dissolving Buffer (ADB) to the gel slice containing a DNA sample, let dissolve, and then transfer to the supplied Zymo-Spin™ IC-XL Column. There is no need for organic denaturants or chloroform. Instead, the product utilizes unique spin column technology to yield high-quality, purified DNA in just minutes. DNA purified using the Zymoclean™ Large Fragment DNA Recovery Kit is ideal for PCR, sequencing, endonuclease digestion, ligation, etc. The entire procedure typically takes about 15 minutes.
DNA recovery (lanes 1-6) from HindIII-digested lambda DNA (lane C) and lambda DNA (λ) using the Zymoclean™ Large Fragment DNA Recovery Kit. Lanes 1, 3: 23 kb and 2, 4: 9 kb bands, respectively. Lanes 5, 6: ~48 kb bands.
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Product |
Catalog No. |
Size |
Price(Price) |
Protocol |
Zymoclean™ Large Fragment DNA Recovery Kit |
D4045 |
25 Preps. |
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D4046 |
100 Preps. |
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