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RNA |
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| RNA>RNA Clean-up |
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ZR-96 RNA Clean & Concentrator™ (A-R1080) |
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ZR-96 RNA Clean & Concentrator™
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Quick (20 minute) high-throughput (96-well) method for cleaning and concentrating RNA. |
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Fast-Spin plate technology allows RNA to be eluted into minimal volumes (≥10 µl). |
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Eluted RNA is ultra clean and ready for subsequent analysis and molecular manipulation. |
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Format: 96-well Plate |
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Sample Sources: RNA fragments (≥ 17 nucleotides): DNase treated RNA, labeled RNA fragments, in vitro transcription products, etc. |
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RNA Recovery: Typically, RNA is eluted into as little as ≥10 µl RNase-free water allowing for a highly concentrated sample. The RNA binding capacity of the supplied Zymo-Spin™ I-96 Plate is ~25 µg/well. |
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RNA Purity: High quality RNA (A260/A280 > 1.8, A260/A230 > 1.8) suitable for all downstream RNA-based manipulations. |
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RNA Storage: RNA is eluted with RNase-free water and can be stored at ≤ -70°C. The addition of RNase inhibitors is optional but highly recommended for prolonged storage. |
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Equipment Needed: Microcentrifuge |
Descriptions
The ZR-96 RNA Clean & Concentrator™ provides a simple and reliable method for large-scale (96-well) purification and concentration of up to ~25 µg (per well) of high-quality RT-PCR-ready RNA. The procedure is based on the use of a unique single-buffer system and Fast-Spin plate technology.
The procedure features a single-buffer system that allows for efficient RNA adsorption from up to 96 samples onto the matrices of the supplied Zymo-Spin™ I-96 Plate. The RNA is washed twice then eluted and concentrated into ≥10 µl of RNase-free water.
The kit allows for small RNA recovery or removal by alternating the first two steps of the purification procedure. RNA fragments (≥17 bases) can be safely treated and recovered using this kit. The result is highly-concentrated purified RNA that is suitable for subsequent RNA-based methods including RT-PCR, hybridization, etc.
The entire RNA isolation procedure typically takes about 20 minutes.
| Product |
Catalog No. |
Size |
Price(RMB) |
Protocol |
| ZR-96 RNA Clean & Concentrator™ |
R1080 |
2x 96 Preps. |
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