DNA-Free RNA Kit™ provides a simple and reliable method for the rapid preparation of up to ~5 μg (per prep.) of high-quality RT-PCR-ready, DNA-free RNA. This simple procedure is based on the use of a unique single-buffer system and Fast-Spin column technology. The procedure is easy: treat your RNA sample with DNase I, add binding buffer, adjust the conditions by adding ethanol, and the cleaned RNA is then concentrated into ≥6 μl of RNase-free water using the Zymo-Spin™ IC Column. RNA fragments (≥17 bases) can be safely treated and recovered using this kit. The result is highlyconcentrated, purified RNA that is suitable for subsequent RNA-based methods including RT-PCR, hybridization, etc. The purification procedure after the DNase treatment typically takes about 5 minutes.

Purification of RNA samples containing genomic and plasmid DNA by using the DNA-Free RNA Kit™. (1) RNA/DNA sample purified without prior DNase treatment, (2) DNase treated, purified RNA sample.