Genomic DNA Clean & Concentrator™
| • |
Quick (5 minute) spin column recovery of large-sized DNA (e.g., genomic, mitochondrial, plasmid
(BAC/PAC), viral, phage, (wga)DNA, etc.) from any enzymatic reaction or impure preparation (e.g.,
Proteinase K digestion). No messy precipitations! |
| • |
Unique spin column for low volume (≥10 μl) elution of ultra-pure, high-yield DNA. |
| • |
Eluted DNA is ideal for PCR, endonuclease digestion, sequencing, etc. |
| • |
Format: Spin Column (capped) |
| • |
Sample Sources: DNA from impure preparations of genomic DNA (e.g., Proteinase K digestions), plasmid DNA (including BAC), viral DNA, and whole genome amplified (wga) DNA.
Can also be used for the purification of lower molecular weight DNA (50 bp to 10 kb)
from PCR, endonuclease digestion, post-RT cDNA synthesis, etc.
|
| • |
DNA Size Limits: 50 bp to ~200 kb |
| • |
DNA Recovery: Typically, up to 10 μg total DNA per column can be eluted with ≥10 μl water. For DNA 50 bp to 200 kb the typical recovery is 70-95%.
|
| • |
DNA Purity: Ultra-pure (A(260/280) ≥1.8) high molecular weight DNA is eluted in water and is especially well suited for PCR, sequencing, endonuclease digestion, etc. |
| • |
Detergent Tolerance: ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS |
| • |
Equipment Needed: Microcentrifuge |
Descriptions
The Genomic DNA Clean & Concentrator™ (DCC™) is for the quick (5 minute) recovery of ultra-pure, large-sized DNA (e.g., genomic, mitochondrial, plasmid(BAC/PAC), viral, phage, (wga) DNA, etc.) from any enzymatic reaction or impurepreparation (e.g., Proteinase K digestion). There is no need for organic denaturants,chloroform, or messy precipitations: simply add the specially formulated ChIP DNA Binding Buffer to a sample and then transfer the mixture to the supplied Zymo-Spin™ Column. Eluted DNA is suitable for sequencing, PCR, endonuclease digestion, and other enzymatic procedures. The product is also compatible with smaller DNAs (50 bp to 10 kb) from PCR, digestions, crude plasmid preparations, cDNA synthesis, etc.
|
Figure 1. Lambda phage DNA (48.5 kb) is effectively recovered from various concentrations of starting material using the Genomic DCC™.
|
|
Figure 2. High molecular weight DNA is efficiently purified using the Genomic DCC™. Porcine gDNA (~35-50 kb), T4 phage DNA (170 kb), and lambda phage DNA (48.5 kb) were purified (in duplicate) from input material using the Genomic DCC™. Eluted DNAs were analyzed in a 0.8% (w/v) TAE/agarose/EtBr gel (shown above). The size marker “M” is a 1 kb ladder (Zymo Research).
|
| Product |
Catalog No. |
Size |
Price(RMB) |
Protocol |
| Genomic DNA Clean & Concentrator™ |
A-D4010 |
25 Preps. |
 |
|
| |
A-D4011 |
100 Preps. |
|
|
| |
表观遗传学产品全面解决方案列下：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| DNA羟甲基化定量试剂盒(荧光法) |
48 次 |
PDF |
A-P-1037-48 |
 |
| DNA羟甲基化定量试剂盒(荧光法) |
96 次 |
PDF |
A-P-1037-96 |
|
| DNA羟甲基化定量试剂盒(比色法) |
48 次 |
PDF |
A-P-1036-48 |
|
| DNA羟甲基化定量试剂盒(比色法) |
96 次 |
PDF |
A-P-1036-96 |
|
| DNA甲基化定量试剂盒(荧光法) |
48 次 |
PDF |
A-P-1035-48 |
|
| DNA甲基化定量试剂盒(荧光法) |
96 次 |
PDF |
A-P-1035-96 |
|
| DNA甲基化定量试剂盒(比色法) |
48 次 |
PDF |
A-P-1034-48 |
|
| DNA甲基化定量试剂盒(比色法) |
96 次 |
PDF |
A-P-1034-96 |
|
| 通用DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1011-48 |
|
| 通用DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1011-96 |
|
| 超敏DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1021-48 |
|
| 超敏DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1021-96 |
|
| 总DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1014-48 |
|
| 总DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1014-96 |
|
推荐阅读：
关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶，并且区分5-hmC, 5-mC, 和 C，使得研究者能够重新评估他们的DNA甲基化信息，也能够在新样品中寻找DNA羟甲基化。 |
