鼠尾直接PCR裂解试剂 货号:A-102-T
DirectPCR Lysis Reagent (mouse tail)
背景资料:Viagen DirectPCR® DNA Extraction System is a single-tube system for rapid preparation of DNA from mouse tails, ear pieces, yolk sac, and culture cells. The patent-pending components developed by scientists at Viagen Biotech Inc. allow the resulting DNA extracts to be compatible with genomic PCR for genotyping. Crude extracts of biological samples are not compatible with many molecular biology-grade reactions such as polymerase chain reaction (PCR), in part due to inhibitors contained in crude extracts. The DirectPCR reagents not only mediate the rapid lysis of biological samples but also contain inhibitors that effectively suppress the inhibitory activities of crude lysates for PCR amplification, while maximally maintaining the integrity of released genomic DNA. The patent-pending simple procedure completely eliminate any solution transfer or tube-opening steps, providing you with substancial extra time.
产品描述:
1. Lyse tails in DirectPCR® Lysis Reagent.
2. Incubate for 45 min at 85°C.
3. PCR genotyping with 1 ml lysates)
产品特点:
Detailed protocols: Tail, Ear, Yolk Sac, and Cultured cells.
DirectPCR® system offers advantages over conventional protocols that include:
· Time saving: Virtually no hands-on time. Crude tail lysates for PCR!
· Money saving:
· Safe: No organic reagents.
· Environmental: No wastes (organic reagents, tubes, tips, etc...)
· Reliable and efficient: Virtually 100% success rate with high yields
保存建议:在接收到艾德寄出的试剂盒后请按照说明书建议,使用不同的保存条件或温度来保存试剂盒内组分
定购信息:
产品名称 |
规格 |
操作手册 |
货号 |
询价 |
鼠尾直接PCR裂解试剂 |
100 ml |
PDF |
A-102-T |
|
鼠尾直接PCR裂解试剂 |
50 ml |
PDF |
A-101-T |
|
肝素抗凝血直接PCR试剂盒 |
50 次 |
PDF |
A-TP01231 |
|
肝素抗凝血直接PCR试剂盒 |
100 次 |
PDF |
A-TP01232 |
|
肝素抗凝血直接PCR试剂盒 |
500 次 |
PDF |
A-TP01233 |
|
肝素抗凝血直接PCR试剂盒 |
2000 次 |
PDF |
A-TP01234 |
|
全血/血清直接PCR试剂盒 |
200 次 |
PDF |
A-P7605-200 |
|
表观遗传学产品全面解决方案列下:
产品名称 |
规格 |
操作手册 |
货号 |
询价 |
DNA羟甲基化定量试剂盒(比色法) |
48 次 |
PDF |
A-P-1035-48 |
|
DNA羟甲基化定量试剂盒(比色法) |
96 次 |
PDF |
A-P-1035-96 |
|
DNA甲基化定量试剂盒(荧光法) |
48 次 |
PDF |
A-P-1036-48 |
|
DNA甲基化定量试剂盒(荧光法) |
96 次 |
PDF |
A-P-1036-96 |
|
DNA甲基化定量试剂盒(比色法) |
48 次 |
PDF |
A-P-1034-48 |
|
DNA甲基化定量试剂盒(比色法) |
96 次 |
PDF |
A-P-1034-96 |
|
通用DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1011-48 |
|
通用DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1011-96 |
|
超敏DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1021-48 |
|
超敏DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1021-96 |
|
总DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1014-48 |
|
总DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1014-96 |
|
推荐阅读:
关于5-hmC
5-hmC是近年来在动物组织中发现的,由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能,有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息,也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前,我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序,这些技术都不适合用来检测5-hmC,因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题,EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶,并且区分5-hmC, 5-mC, 和 C,使得研究者能够重新评估他们的DNA甲基化信息,也能够在新样品中寻找DNA羟甲基化。 |