Roche 480 SYBR快速定量试剂盒                                                                    货号：A-KK4609

KAPA SYBR® FAST Roche LightCycler® 480 2X qPCR Master Mix

背景资料：The KAPA SYBR® DNA Polymerase was engineered to perform optimally in stringent real-time qPCR reaction conditions, exhibiting dramatic improvements to signal-to-noise ratio (fluorescence), cycle threshold (Ct), linearity, and sensitivity. The KAPA SYBR® DNA Polymerase and proprietary buffer system enhances the amplification efficiency of difficult templates, including both GC-rich and AT-rich templates.

产品描述：KAPA SYBR® FAST qPCR Kits are supplied as a ready-to-use cocktail containing all components except primers and template, for the amplification and detection of DNA in qPCR..

The KAPA SYBR® FAST qPCR Kit is supplied as a 2x Master Mix with integrated antibody-mediated hot start, SYBR® Green I fluorescent dye, MgCl2, dNTPs, and stabilizers. ROX reference dye is included in the ABI Prism® Master Mix (2X), and supplied in separate tubes in the Universal Master Mix (2X). Fluorescein reference dye is included in the Bio-Rad iCycler Master Mix (2x). The KAPA SYBR® FAST qPCR Master Mix (2X) optimized for the Roche LightCycler® 480 does not contain reference dye

产品特点：

1· High performance gene expression analysis ；The improved speed, processivity and robustness of KAPA SYBR® FAST qPCR Kits results in consistently high amplification efficiencies required for accurate relative quantification. To demonstrate the high performance of KAPA SYBR® FAST for gene expression analysis, the reaction efficiencies obtained for ten commonly used housekeeping genes in the human breast cancer cell line, MCF-7, were compared. The KAPA SYBR® FAST qPCR Kit achieved consistently high amplification efficiencies (95 – 104%) across all ten genes, despite differences in amplicon length

2·Superior signals, Ct values, and reaction efficiencies；The high performance of the KAPA SYBR® FAST qPCR kit can been seen when compared to other leading Fast qPCR Mater Mixes. KAPA SYBR® FAST consistently produces higher signal, earlier Cts, and 100% reaction efficiencies

3·High speed and high performance；High speed does not compromise performance with the KAPA SYBR® FAST qPCR kits. As can been seen from the amplification plots and standard curves there is no loss in performance between fast and conventional cycling conditions even with complex targets

4.Highest performance on Roche LightCycler® 480。

5.Highest fluorescence and earliest Ct.

保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分

定购信息：

产品名称	规格	操作手册	货号	询价
Roche 480 SYBR快速定量试剂盒	1 ml	PDF	A-KK4609
Roche 480 SYBR快速定量试剂盒	5 ml	PDF	A-KK4610
Roche 480 SYBR快速定量试剂盒	10 ml	PDF	A-KK4611
Bio-Rad SYBR快速定量试剂盒	1 ml	PDF	A-KK4606
Bio-Rad SYBR快速定量试剂盒	5 ml	PDF	A-KK4607
Bio-Rad SYBR快速定量试剂盒	10 ml	PDF	A-KK4608
ABI Prism SYBR快速定量试剂盒	1 mL	PDF	A-KK4603
ABI Prism SYBR快速定量试剂盒	5 mL	PDF	A-KK4604
ABI Prism SYBR快速定量试剂盒	10 mL	PDF	A-KK4605
Paq5000 Hotstart PCR Master Mix	400 次	PDF	A-600872
Paq5000 Hotstart PCR Master Mix	100 次	PDF	A-600870
植物DNA小量提取试剂盒	50 次	PDF	A-DH813-01
植物DNA小量提取试剂盒	200 次	PDF	A-DH813-02
凝胶DNA纯化回收试剂盒	100 次	PDF	A-D2110-01
凝胶DNA纯化回收试剂盒	200 次	PDF	A-D2110-02
凝胶DNA纯化回收试剂盒	500 次	PDF	A-D2110-03
凝胶DNA纯化回收试剂盒	1000次	PDF	A-D2110-04
胶自动纯化回收试剂盒(磁珠法)	150 次	PDF	A-DM136-01
胶自动纯化回收试剂盒(磁珠法)	500 次	PDF	A-DM136-02
总RNA自动化富集纯化mRNA试剂盒(磁珠法)	30 次	PDF	A-RM911-01
植物自动化DNA大量提取试剂盒(磁珠法)	50 次	PDF	A-DM311-01
组织切片DNA极速提取试剂盒	100 次	PDF	A-P-1003-100

表观遗传学产品全面解决方案列下：

产品名称	规格	操作手册	货号	询价
DNA羟甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1037-48
DNA羟甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1037-96
DNA羟甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1036-48
DNA羟甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1036-96
DNA甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1035-48
DNA甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1035-96
DNA甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1034-48
DNA甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1034-96
通用DNA甲基化定量试剂盒	48 次	PDF	A-P-1011-48
通用DNA甲基化定量试剂盒	96 次	PDF	A-P-1011-96
超敏DNA甲基化定量试剂盒	48 次	PDF	A-P-1021-48
超敏DNA甲基化定量试剂盒	96 次	PDF	A-P-1021-96
总DNA甲基化定量试剂盒	48 次	PDF	A-P-1014-48
总DNA甲基化定量试剂盒	96 次	PDF	A-P-1014-96

推荐阅读：

关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC

5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶，并且区分5-hmC, 5-mC, 和 C，使得研究者能够重新评估他们的DNA甲基化信息，也能够在新样品中寻找DNA羟甲基化。