通用型一步法SYBR Fast 试剂盒                                                                      货号：A-KK4650

KAPA SYBR® FAST One-Step qRT-PCR Universal

背景资料：The KAPA SYBR® FAST One-Step qRT-PCR Kit is a sensitive and convenient solution for real-time PCR using RNA as template. The kit is composed of KAPA SYBR® FAST Master Mix (2X), KAPA RT Mix (50X) and dUTP (10 mM). The KAPA RT Mix (50X) is comprised of wild-type M-MuLV Reverse Transcriptase and RNase Inhibitor and is optimized for rapid one-step, one-tube RNA quantification.

产品描述：

The KAPA SYBR® FAST Master Mix (2X) contains a novel DNA polymerase engineered via molecular evolution and optimized for qPCR using SYBR® Green I dye chemistry. The 2X Master Mix is a ready-to-use cocktail containing all components except primers and template for the amplification and detection of cDNA. KAPA SYBR® FAST One-Step qRT-PCR Universal kits contain ROX reference dye supplied separately. KAPA SYBR® FAST One-Step qRT-PCR ABI Prism kits contain 2X Master Mix with ROX reference dye included in the mix. KAPA SYBR® FAST One-Step qRT-PCR Bio-Rad iCycler Kits contain 2X Master Mix with fluorescein reference dye included in the mix. 

dUTP (10 mM) is also supplied in the kit. The use of dUTP at the recommended final concentration, results in amplicons that can be degraded using Uracil-DNA Glycosylase (UDG). UDG treatment is performed in subsequent reactions in order to minimize carry over PCR contamination downstream. Use of dUTP in this system is optional. UDG is not supplied in this kit.

产品特点：

1· Superior signal and reaction efficiency；The improved speed, processivity and robustness of KAPA SYBR® FAST One-Step qRT-PCR kits results in consistently high amplification efficiencies required for accurate relative quantification. To demonstrate the high performance of KAPA SYBR® FAST One-Step qRT-PCR Kit for gene expression analysis, the kit was compared to leading competitor kits across a wide range of input RNA concentrations. The KAPA SYBR® FAST One-Step qRT-PCR Kit achieved higher fluorescence, earlier Ct values and improved amplification efficiency when compared to competitor one-step qRT-PCR kits

2·Robust amplification across a broad range of target lengths；To demonstrate the robust performance of the KAPA SYBR® FAST One-Step qRT-PCR Kit, MS2 bacteriophage RNA targets of increasing length were amplified at a concentration of 0.8 pg/20 μl using the KAPA SYBR® FAST One-Step qRT-PCR Kit (green) or Competitor T (red), Competitor I (blue), and Competitor Q (purple). Increased amplicon length led to a significant decrease in competitor performance as indicated by delayed Ct values or complete reaction failure. cDNA synthesis and qPCR amplification were performed using the competitors recommend protocols on the Eppendorf® MasterCycler™ ep realplex4 S real-time PCR cycler

3·High reproducibility and sensitivity across a broad range of concentrations；The high reproducibility and sensitivity of the KAPA SYBR® FAST One-Step qRT-PCR Kit enable accurate interrogation of a broad range of RNA template concentrations. A 279 bp fragment was amplified in replicates of six from log-fold serial dilutions of commercial MS2 bacteriophage RNA (80 pg - 0.8 fg/20 μl). cDNA synthesis was performed at 42 oC for 5 min followed by 95 ºC for 5 min and 40 cycles of 3 sec denaturation at 95 ºC and 30 sec combined annealing/extension at 60 ºC. The calculated reaction efficiency of 95% was obtained using all six replicates per data point. Reactions were performed using the competitors recommend protocols on the Eppendorf® MasterCycler™ ep realplex4 S real-time PCR cycler.

保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分

定购信息：

产品名称	规格	操作手册	货号	询价
通用型一步法SYBR Fast 试剂盒	1 ml	PDF	A-KK4650
通用型一步法SYBR Fast 试剂盒	5 ml	PDF	A-KK4651
通用型一步法SYBR Fast 试剂盒	10 ml	PDF	A-KK4652
Bio-Rad SYBR快速定量试剂盒	1 ml	PDF	A-KK4606
Bio-Rad SYBR快速定量试剂盒	5 ml	PDF	A-KK4607
Bio-Rad SYBR快速定量试剂盒	10 ml	PDF	A-KK4608
ABI Prism SYBR快速定量试剂盒	1 mL	PDF	A-KK4603
ABI Prism SYBR快速定量试剂盒	5 mL	PDF	A-KK4604
ABI Prism SYBR快速定量试剂盒	10 mL	PDF	A-KK4605
Paq5000 Hotstart PCR Master Mix	400 次	PDF	A-600872
Paq5000 Hotstart PCR Master Mix	100 次	PDF	A-600870
植物DNA小量提取试剂盒	50 次	PDF	A-DH813-01
植物DNA小量提取试剂盒	200 次	PDF	A-DH813-02
凝胶DNA纯化回收试剂盒	100 次	PDF	A-D2110-01
凝胶DNA纯化回收试剂盒	200 次	PDF	A-D2110-02
凝胶DNA纯化回收试剂盒	500 次	PDF	A-D2110-03
凝胶DNA纯化回收试剂盒	1000次	PDF	A-D2110-04
胶自动纯化回收试剂盒(磁珠法)	150 次	PDF	A-DM136-01
胶自动纯化回收试剂盒(磁珠法)	500 次	PDF	A-DM136-02
总RNA自动化富集纯化mRNA试剂盒(磁珠法)	30 次	PDF	A-RM911-01
植物自动化DNA大量提取试剂盒(磁珠法)	50 次	PDF	A-DM311-01
组织切片DNA极速提取试剂盒	100 次	PDF	A-P-1003-100

表观遗传学产品全面解决方案列下：

产品名称	规格	操作手册	货号	询价
DNA羟甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1037-48
DNA羟甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1037-96
DNA羟甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1036-48
DNA羟甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1036-96
DNA甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1035-48
DNA甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1035-96
DNA甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1034-48
DNA甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1034-96
通用DNA甲基化定量试剂盒	48 次	PDF	A-P-1011-48
通用DNA甲基化定量试剂盒	96 次	PDF	A-P-1011-96
超敏DNA甲基化定量试剂盒	48 次	PDF	A-P-1021-48
超敏DNA甲基化定量试剂盒	96 次	PDF	A-P-1021-96
总DNA甲基化定量试剂盒	48 次	PDF	A-P-1014-48
总DNA甲基化定量试剂盒	96 次	PDF	A-P-1014-96

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时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶，并且区分5-hmC, 5-mC, 和 C，使得研究者能够重新评估他们的DNA甲基化信息，也能够在新样品中寻找DNA羟甲基化。