第二代超强DNA聚合酶                                                                     货号：A-KK5023

KAPA2G Robust DNA Polymerase

背景资料：The KAPA2G Robust DNA Polymerase is a highly robust and versatile second-generation enzyme derived through a process of molecular evolution. The novel amino acid mutations in KAPA2G Robust DNA Polymerase offer higher processivity and specific activity, which translates to robust performance across a wide range of GC- and AT-rich templates and amplicons, as well as improved tolerance to common PCR inhibitors.

In the HotStart formulation, the enzyme is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step. This eliminates spurious amplification products resulting from non-specific priming events during reaction setup and initiation, and increases overall reaction efficiency.

产品描述：KAPA2G Robust Kits supplied with KAPA2G Buffer A and KAPA2G Buffer B and the proprietary additive, KAPA Enhancer 1, offer extended optimization options for diverse and difficult templates. Kits also contain KAPA2G GC Buffer, a novel buffer formulated specifically for GC-rich templates and amplicons. Like wild-type Taq, KAPA2G Robust DNA Polymerase has 5’-3’ polymerase and exonuclease activities, but no 3’-5’ exonuclease (proofreading activity). The fidelity of KAPA2G Robust DNA Polymerase is similar to that of wild-type Taq

产品特点：

1· Consolidate PCR protocols and increase success rates with a single enzyme；The improved processivity and tolerance to common PCR inhibitors of the KAPA2G Robust DNA Polymerase offers consistent amplification, high yields and wide coverage of both easy and challenging amplicons. The unique features of the enzyme supports versatile and robust amplification of a broad range of AT- and GC-rich targets and allows for the simplification of PCR workflows, through the consolidation of reagents and protocols, while increasing success rates and turnaround time

2·Greatly improved tolerance to common PCR inhibitors；KAPA2G Robust DNA Polymerase was engineered for high performance in chemically complex reaction conditions. The result is superior tolerance to a wide range of common PCR inhibitors, when compared to wild-type Taq polymerase and so-called “robust” polymerase blends. In addition to the examples below, KAPA2G Robust HotStart DNA Polymerase shows improved tolerance to other inhibitors, including KCl, sodium acetate, isopropanol and phenol (at high template and enzyme concentrations).

3·Unrivalled performance in Colony PCR；The improved inhibitor tolerance of KAPA2G Robust translates into unrivalled performance in Colony PCR, a PCR application that is prone to failure and inconsistency due to the presence of inhibitors. With the KAPA2G Robust PCR kit, a 100% success rate in Colony PCR is achievable, starting from plated bacterial colonies or overnight cultures

4.The higher processivity and specific activity of KAPA2G Robust DNA Polymerase results in higher yields per unit of enzyme, which often translates into improved sensitivity. Higher yields and sensitivity are also achieved through the use of novel buffers, which have been formulated for optimal annealing specificity. For certain assays, specificity and efficiency may be improved further through the use of KAPAEnhancer 1 in combination with KAPA2G Buffer A or KAPA2G Buffer B.

保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分

定购信息：

产品名称	规格	操作手册	货号	询价
第二代超强DNA聚合酶	100 U	PDF	A-KK5023
第二代超强DNA聚合酶	250 U	PDF	A-KK5024
Bio-Rad SYBR快速定量试剂盒	1 ml	PDF	A-KK4606
Bio-Rad SYBR快速定量试剂盒	5 ml	PDF	A-KK4607
Bio-Rad SYBR快速定量试剂盒	10 ml	PDF	A-KK4608
ABI Prism SYBR快速定量试剂盒	1 mL	PDF	A-KK4603
ABI Prism SYBR快速定量试剂盒	5 mL	PDF	A-KK4604
ABI Prism SYBR快速定量试剂盒	10 mL	PDF	A-KK4605
Paq5000 Hotstart PCR Master Mix	400 次	PDF	A-600872
Paq5000 Hotstart PCR Master Mix	100 次	PDF	A-600870
植物DNA小量提取试剂盒	50 次	PDF	A-DH813-01
植物DNA小量提取试剂盒	200 次	PDF	A-DH813-02
凝胶DNA纯化回收试剂盒	100 次	PDF	A-D2110-01
凝胶DNA纯化回收试剂盒	200 次	PDF	A-D2110-02
凝胶DNA纯化回收试剂盒	500 次	PDF	A-D2110-03
凝胶DNA纯化回收试剂盒	1000次	PDF	A-D2110-04
胶自动纯化回收试剂盒(磁珠法)	150 次	PDF	A-DM136-01
胶自动纯化回收试剂盒(磁珠法)	500 次	PDF	A-DM136-02
总RNA自动化富集纯化mRNA试剂盒(磁珠法)	30 次	PDF	A-RM911-01
植物自动化DNA大量提取试剂盒(磁珠法)	50 次	PDF	A-DM311-01
组织切片DNA极速提取试剂盒	100 次	PDF	A-P-1003-100

表观遗传学产品全面解决方案列下：

产品名称	规格	操作手册	货号	询价
DNA羟甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1037-48
DNA羟甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1037-96
DNA羟甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1036-48
DNA羟甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1036-96
DNA甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1035-48
DNA甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1035-96
DNA甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1034-48
DNA甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1034-96
通用DNA甲基化定量试剂盒	48 次	PDF	A-P-1011-48
通用DNA甲基化定量试剂盒	96 次	PDF	A-P-1011-96
超敏DNA甲基化定量试剂盒	48 次	PDF	A-P-1021-48
超敏DNA甲基化定量试剂盒	96 次	PDF	A-P-1021-96
总DNA甲基化定量试剂盒	48 次	PDF	A-P-1014-48
总DNA甲基化定量试剂盒	96 次	PDF	A-P-1014-96

推荐阅读：

关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC

5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶，并且区分5-hmC, 5-mC, 和 C，使得研究者能够重新评估他们的DNA甲基化信息，也能够在新样品中寻找DNA羟甲基化。