第二代快速DNA热启动聚合酶                                                                    货号：A-KK5523

KAPA2G Fast HotStart DNA Polymerase

背景资料：KAPA2G Fast DNA Polymerase, a second-generation enzyme derived through a process of molecular evolution. KAPA2G Fast DNA Polymerase was specifically engineered for higher processivity and speed, offering significantly faster extension rates than wild-type Taq polymerase. In the HotStart formulation, the enzyme is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step. This eliminates spurious amplification products resulting from non-specific priming events during reaction setup and initiation, and increases overall reaction efficiency.

产品描述：KAPA2G Fast HotStart is particularly well suited for high throughput Fast PCR, offering total reaction times 20 - 70% shorter than conventional PCR assays performed with wild-type Taq polymerase or hot start formulations thereof. In addition, KAPA2G Fast HotStart has been shown to achieve higher yields and sensitivity than competitor enzymes across a wide range of amplicon types. No specialized PCR consumables or thermocyclers are required.

产品特点：

1· The ultimate in speed and performance；KAPA2G Fast HotStart and optimized buffer system is capable of dramatically reduced extension times and exceptional performance.Hot start formulations of wild-type Taq do not perform satisfactorily when cycling times are reduced. In contrast, the unique properties of KAPA2G Fast HotStart allows for efficient amplification in total reaction times that are typically reduced by 40 - 70%

2·High speed and sensitivity；KAPA2G Fast HotStart is based on a second-generation polymerase with the intrinsic ability to synthesize DNA faster than wild-type Taq and other DNA polymerases. Fast PCR protocols using KAPA2G Fast HotStart are primarily based on reduced extension times that allow for a significant reduction in PCR cycling time without the risk of compromising reaction performance.Using KAPA2G Fast HotStart, a total extension time of 1 second per cycle is sufficient for the reproducible amplification of fragments <1 kb from as little as a single target copy of human genomic DNA. For amplicons >1 kb, 15 sec/kb extension time per cycle is used

3·Fast, more sensitive Multiplex PCR；With KAPA2G Fast HotStart, faster Multiplex PCR is also possible. Because of the complexity of multiplex assays, longer annealing times are maintained, but extension times may be reduced to 10 sec per cycle for fragments <1 kb. Extension at 65ºC instead of 72ºC may also improve results. With optimized KAPA2G Fast HotStart protocols, time savings of 35 - 70% can be achieved.Multiplex PCR assays typically employ a high template concentration. Using KAPA2G Fast HotStart in 1.5x KAPA2G HotStart buffer, improved sensitivity was achieved in all model systems tested .

保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分

定购信息：

产品名称	规格	操作手册	货号	询价
第二代快速DNA热启动聚合酶	100 U	PDF	A-KK5523
第二代快速DNA热启动聚合酶	250 U	PDF	A-KK5503
第二代快速DNA热启动聚合酶	500 U	PDF	A-KK5501
第二代快速DNA热启动聚合酶	2500 U	PDF	A-KK5519
Bio-Rad SYBR快速定量试剂盒	1 ml	PDF	A-KK4606
Bio-Rad SYBR快速定量试剂盒	5 ml	PDF	A-KK4607
Bio-Rad SYBR快速定量试剂盒	10 ml	PDF	A-KK4608
ABI Prism SYBR快速定量试剂盒	1 mL	PDF	A-KK4603
ABI Prism SYBR快速定量试剂盒	5 mL	PDF	A-KK4604
ABI Prism SYBR快速定量试剂盒	10 mL	PDF	A-KK4605
Paq5000 Hotstart PCR Master Mix	400 次	PDF	A-600872
Paq5000 Hotstart PCR Master Mix	100 次	PDF	A-600870
植物DNA小量提取试剂盒	50 次	PDF	A-DH813-01
植物DNA小量提取试剂盒	200 次	PDF	A-DH813-02
凝胶DNA纯化回收试剂盒	100 次	PDF	A-D2110-01
凝胶DNA纯化回收试剂盒	200 次	PDF	A-D2110-02
凝胶DNA纯化回收试剂盒	500 次	PDF	A-D2110-03
凝胶DNA纯化回收试剂盒	1000次	PDF	A-D2110-04
胶自动纯化回收试剂盒(磁珠法)	150 次	PDF	A-DM136-01
胶自动纯化回收试剂盒(磁珠法)	500 次	PDF	A-DM136-02
总RNA自动化富集纯化mRNA试剂盒(磁珠法)	30 次	PDF	A-RM911-01
植物自动化DNA大量提取试剂盒(磁珠法)	50 次	PDF	A-DM311-01
组织切片DNA极速提取试剂盒	100 次	PDF	A-P-1003-100

表观遗传学产品全面解决方案列下：

产品名称	规格	操作手册	货号	询价
DNA羟甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1037-48
DNA羟甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1037-96
DNA羟甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1036-48
DNA羟甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1036-96
DNA甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1035-48
DNA甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1035-96
DNA甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1034-48
DNA甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1034-96
通用DNA甲基化定量试剂盒	48 次	PDF	A-P-1011-48
通用DNA甲基化定量试剂盒	96 次	PDF	A-P-1011-96
超敏DNA甲基化定量试剂盒	48 次	PDF	A-P-1021-48
超敏DNA甲基化定量试剂盒	96 次	PDF	A-P-1021-96
总DNA甲基化定量试剂盒	48 次	PDF	A-P-1014-48
总DNA甲基化定量试剂盒	96 次	PDF	A-P-1014-96

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时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶，并且区分5-hmC, 5-mC, 和 C，使得研究者能够重新评估他们的DNA甲基化信息，也能够在新样品中寻找DNA羟甲基化。