血液直接扩增试剂盒B                                                                      货号：A-KK7003

KAPA Blood PCR Mix B

背景资料：KAPA Blood PCR Kits are available in two optimized, easy-to-use 2x formats containing all PCR components except primers and template (whole blood).

产品描述：KAPA Blood PCR Mix A is preferred for assays employing highly sensitive fluorescent detection systems. KAPA Blood Mix B is formulated for higher yields and GC-rich amplicons and is recommended when analysis is by agarose gel electrophoresis and ethidium bromide staining. Products generated with KAPA Blood PCR Mixes A or B may be analyzed by restriction endonuclease digestion, DNA sequencing or dHPLC analysis.

产品特点：

1· Reduce contamination risk, turnaround time and cost of genetic testing；High-throughput genetic testing directly from blood has not been feasible with wild-type polymerases, due to the presence of multiple PCR inhibitors in whole blood. KAPA Blood DNA Polymerase was evolved in this environment and offers the ability to amplify DNA fragments directly from blood, without any pretreatment of blood samples or DNA isolation. This not only reduces the cost and turnaround time of genetic testing, but also significantly reduces the risk of sample cross-contamination associated with the need for DNA extraction

2·Compatibility with existing workflows and detection methods；KAPA Blood DNA Polymerase is supplied in two optimized, convenient 2x PCR Mixes, which are easily integrated with existing protocols and detection methods. Reaction products are centrifuged to recover amplified DNA from hematocyte debris

3·Paternity testing using fluorescent capillary electrophoresis；Typical result obtained in a PowerPlex16® (Promega Corporation) paternity test using KAPA Blood PCR Mix A, with blood collected on Whatman 903® Specimen Collection Paper (“Guthrie cards”) as template. The test is based on the amplification of 16 loci (15 STR loci and amelogenin) in a single Multiplex PCR using primers labelled with three different fluorophores. Reactions were set up and performed according to manufacturers’ recommendations. Reaction products were centrifuged and cleared supernatants were analyzed directly by fluorescent capillary electrophoresis using standard protocols. Image courtesy of Unistel Medical Laboratories

4.Genetic testing based on RE digestion of DNA fragments amplified directly from whole blood;

5.Direct amplification from the blood of non-human species;KAPA Blood PCR Kits may also be used for the direct amplification of DNA from the blood of non-human species, such as other mammals and birds, thereby offering cost and time savings in PCR-based veterinary testing. Blood from other species may be collected in the same manner as human blood. The optimal amount of blood used in a KAPA Blood PCR must be determined empirically for each species. With mouse blood, best results have been obtained with a lower concentration of blood in the PCR (0.1 - 5% v/v) than recommended for humans. For bird species with nucleated erythrocytes, blood should be diluted to more closely approximate the DNA concentration in the same volume of human blood.

保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分

定购信息：

产品名称	规格	操作手册	货号	询价
血液直接扩增试剂盒B	500 rxn	PDF	A-KK7003
血液直接扩增试剂盒B	1000 rxn	PDF	A-KK7005
Bio-Rad SYBR快速定量试剂盒	1 ml	PDF	A-KK4606
Bio-Rad SYBR快速定量试剂盒	5 ml	PDF	A-KK4607
Bio-Rad SYBR快速定量试剂盒	10 ml	PDF	A-KK4608
ABI Prism SYBR快速定量试剂盒	1 mL	PDF	A-KK4603
ABI Prism SYBR快速定量试剂盒	5 mL	PDF	A-KK4604
ABI Prism SYBR快速定量试剂盒	10 mL	PDF	A-KK4605
Paq5000 Hotstart PCR Master Mix	400 次	PDF	A-600872
Paq5000 Hotstart PCR Master Mix	100 次	PDF	A-600870
植物DNA小量提取试剂盒	50 次	PDF	A-DH813-01
植物DNA小量提取试剂盒	200 次	PDF	A-DH813-02
凝胶DNA纯化回收试剂盒	100 次	PDF	A-D2110-01
凝胶DNA纯化回收试剂盒	200 次	PDF	A-D2110-02
凝胶DNA纯化回收试剂盒	500 次	PDF	A-D2110-03
凝胶DNA纯化回收试剂盒	1000次	PDF	A-D2110-04
胶自动纯化回收试剂盒(磁珠法)	150 次	PDF	A-DM136-01
胶自动纯化回收试剂盒(磁珠法)	500 次	PDF	A-DM136-02
总RNA自动化富集纯化mRNA试剂盒(磁珠法)	30 次	PDF	A-RM911-01
植物自动化DNA大量提取试剂盒(磁珠法)	50 次	PDF	A-DM311-01
组织切片DNA极速提取试剂盒	100 次	PDF	A-P-1003-100

表观遗传学产品全面解决方案列下：

产品名称	规格	操作手册	货号	询价
DNA羟甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1037-48
DNA羟甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1037-96
DNA羟甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1036-48
DNA羟甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1036-96
DNA甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1035-48
DNA甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1035-96
DNA甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1034-48
DNA甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1034-96
通用DNA甲基化定量试剂盒	48 次	PDF	A-P-1011-48
通用DNA甲基化定量试剂盒	96 次	PDF	A-P-1011-96
超敏DNA甲基化定量试剂盒	48 次	PDF	A-P-1021-48
超敏DNA甲基化定量试剂盒	96 次	PDF	A-P-1021-96
总DNA甲基化定量试剂盒	48 次	PDF	A-P-1014-48
总DNA甲基化定量试剂盒	96 次	PDF	A-P-1014-96

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5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC

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时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶，并且区分5-hmC, 5-mC, 和 C，使得研究者能够重新评估他们的DNA甲基化信息，也能够在新样品中寻找DNA羟甲基化。