鼠基因分型试剂盒 货号：A-KK7302
KAPA Mouse Genotyping Kit
背景资料：KAPA Mouse Genotyping Kits include KAPA Express Extract, a novel thermostable protease and buffer system that allows the extraction of PCR-ready DNA from mouse tissue in as little as 15 minutes, and KAPA2G Fast Genotyping Mix with dye, containing a DNA polymerase engineered via a process of molecular evolution for high processivity and extreme speed. The combination of KAPA Express Extract and KAPA2G Fast Genotyping Mix allows for the reliable extraction and amplification of DNA fragments from mouse tissue in 1 hour, as compared to ≥1 day with conventional protocols.
产品描述：The KAPA Express Extract system has been designed for optimal tissue lysis and sample preservation. Unlike existing protocols that rely on proteinase K digestion, extractions using KAPA Express Extract are conveniently performed in a single-tube, without the need for hazardous chemicals and multiple washing steps.
产品特点：
1· Current workflows for the extraction and amplification of DNA for mouse genotyping can benefit from improvements in throughput, turnaround time and reliability；Template DNA for mouse genotyping is routinely prepared from ear or tail biopsy samples. Typically, samples are incubated for several hours or overnight with Proteinase K, followed by purification of the DNA to remove salts and detergents. Alternatively, DNA is released from the mouse tissue by heating the sample in an alkaline solution, followed by neutralization in a Tris-HCl buffer with an acidic pH. Proteinase K protocols yield extracts with a higher DNA concentration and quality, but are laborious and time-consuming. Heat lysis protocols allow for rapid generation of PCR-ready DNA, but samples vary in quality and the concentration of amplifiable template, leading to reduced PCR success rates with wild-type DNA polymerases
2·The KAPA Mouse Genotyping Kit outperforms crude DNA extraction methods used for mouse genotyping, in significantly shorter turnaround times；
3·The KAPA Mouse Genotyping Kit performs as well or better than other commercial kits used for mouse genotyping at lower cost.
保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分
定购信息：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| 鼠基因分型试剂盒 |
500 次 |
PDF |
A-KK7302 |
 |
| 鼠基因分型试剂盒 |
500 次 |
PDF |
A-KK7352 |
|
| Bio-Rad SYBR快速定量试剂盒 |
1 ml |
PDF |
A-KK4606 |
|
| Bio-Rad SYBR快速定量试剂盒 |
5 ml |
PDF |
A-KK4607 |
|
| Bio-Rad SYBR快速定量试剂盒 |
10 ml |
PDF |
A-KK4608 |
|
| ABI Prism SYBR快速定量试剂盒 |
1 mL |
PDF |
A-KK4603 |
|
| ABI Prism SYBR快速定量试剂盒 |
5 mL |
PDF |
A-KK4604 |
|
| ABI Prism SYBR快速定量试剂盒 |
10 mL |
PDF |
A-KK4605 |
|
| Paq5000 Hotstart PCR Master Mix |
400 次 |
PDF |
A-600872 |
|
| Paq5000 Hotstart PCR Master Mix |
100 次 |
PDF |
A-600870 |
|
| 植物DNA小量提取试剂盒 |
50 次 |
PDF |
A-DH813-01 |
|
| 植物DNA小量提取试剂盒 |
200 次 |
PDF |
A-DH813-02 |
|
| 凝胶DNA纯化回收试剂盒 |
100 次 |
PDF |
A-D2110-01 |
|
| 凝胶DNA纯化回收试剂盒 |
200 次 |
PDF |
A-D2110-02 |
|
| 凝胶DNA纯化回收试剂盒 |
500 次 |
PDF |
A-D2110-03 |
|
| 凝胶DNA纯化回收试剂盒 |
1000次 |
PDF |
A-D2110-04 |
|
| 胶自动纯化回收试剂盒(磁珠法) |
150 次 |
PDF |
A-DM136-01 |
|
| 胶自动纯化回收试剂盒(磁珠法) |
500 次 |
PDF |
A-DM136-02 |
|
| 总RNA自动化富集纯化mRNA试剂盒(磁珠法) |
30 次 |
PDF |
A-RM911-01 |
|
| 植物自动化DNA大量提取试剂盒(磁珠法) |
50 次 |
PDF |
A-DM311-01 |
|
| 组织切片DNA极速提取试剂盒 |
100 次 |
PDF |
A-P-1003-100 |
|
表观遗传学产品全面解决方案列下：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| DNA羟甲基化定量试剂盒(荧光法) |
48 次 |
PDF |
A-P-1037-48 |
|
| DNA羟甲基化定量试剂盒(荧光法) |
96 次 |
PDF |
A-P-1037-96 |
|
| DNA羟甲基化定量试剂盒(比色法) |
48 次 |
PDF |
A-P-1036-48 |
|
| DNA羟甲基化定量试剂盒(比色法) |
96 次 |
PDF |
A-P-1036-96 |
|
| DNA甲基化定量试剂盒(荧光法) |
48 次 |
PDF |
A-P-1035-48 |
|
| DNA甲基化定量试剂盒(荧光法) |
96 次 |
PDF |
A-P-1035-96 |
|
| DNA甲基化定量试剂盒(比色法) |
48 次 |
PDF |
A-P-1034-48 |
|
| DNA甲基化定量试剂盒(比色法) |
96 次 |
PDF |
A-P-1034-96 |
|
| 通用DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1011-48 |
|
| 通用DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1011-96 |
|
| 超敏DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1021-48 |
|
| 超敏DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1021-96 |
|
| 总DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1014-48 |
|
| 总DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1014-96 |
|
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Extracted DNA is then amplified with KAPA2G Fast Genotyping Mix (2X) with dye, a ready-to-use master mix containing all components for Fast PCR, except primers and template. The master mix is designed for routine Fast PCR and improved performance, compared to wild-type Taq DNA polymerase. The master mix with dye additionally contains two inert dyes, which allow for the analysis of reaction products by gel electrophoresis directly after completion of the PCR, i.e. without the need to add a DNA loading solution. |
