高保真二代测序文库扩增试剂盒 货号：A-KK2611
KAPA HiFi Library Amplification Kit
背景资料：High fidelity PCR is used to selectively enrich library fragments carrying appropriate adaptor sequences and to amplify the amount of DNA prior to sequencing. During PCR enrichment, the polymerase does not synthesize all library fragments with equal efficiency. This amplification bias exacerbates uneven sequence coverage.
产品描述：GC content is known to be an important factor in NGS library amplification bias (Aird et al. Genome Biology 2011, 12:R18). DNA polymerases commonly used for library amplification display significant bias against GC- and AT-rich regions. Genomes with extreme average GC content are particularly susceptible to amplification bias. High-GC content elements such as 5’ UTRs, CpG islands and first exons, contained within the balanced human genome, are also vulnerable to amplification bias (Zhang L et al. PNAS 2004; 101:16855-16860).
产品特点：
1· Effect of high-GC content on coverage depth for libraries amplified using common proof-reading (B-family) polymerases；
2·Percentage of the M. tuberculosis genome not represented in sequence data when using different PCR reagents for library amplification；
3·Low GC-content libraries result in variable bias depending on the polymerase used for amplification；
4.Library amplification can dramatically affect coverage uniformity。
5.Superior accuracy for high fidelity library amplification.
保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分
定购信息：
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规格 |
操作手册 |
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50 rxn |
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A-KK2611 |
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KAPA HiFi Library Amplification Kits have been designed to address PCR-induced bias. Kits contain the novel KAPA HiFi DNA Polymerase, engineered for high fidelity and processivity and capable of balanced amplification of complex library DNA. Kits are supplied as a ready-to-use master mix (2X) containing all components for PCR, except primers and template.
KAPA HiFi HotStart DNA Polymerase is an antibody-based hot start formulation of KAPA HiFi DNA Polymerase, a novel B-family DNA polymerase exhibiting industry-leading performance in comparison with other high-fidelity (B-family) DNA polymerases and polymerase blends. KAPA HiFi DNA Polymerase was engineered for increased affinity to DNA, without the need for accessory protein domains. The intrinsic high processivity of the enzyme results in significant improvements in yield, sensitivity, speed, target length and the ability to amplify difficult amplicons. These enhancements result in lower amplification bias which leads to more uniform sequence coverage. In the HotStart formulation, a proprietary antibody inactivates the polymerase until the first denaturation step. This eliminates spurious amplification products resulting from non-specific priming events during reaction setup and initiation, and increases overall reaction efficiency
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