高保真二代测序文库实时定量扩增试剂盒                                                                    货号：A-KK2701

KAPA Real-Time Library Amplification Kit

背景资料：KAPA Real-Time Library Amplification Kits contain KAPA HiFi HotStart Real-Time PCR Master Mix (2X), a ready-to-use cocktail containing all components for PCR, except primers and template. The 2X Master Mix contains KAPA HiFi HotStart DNA Polymerase in a proprietary reaction buffer, dNTPs, MgCl2 (2.5 mM at 1X), SYBR® Green I dye and stabilizers. Four fluorescent standards are supplied, and are used to define a window for optimal amplification.

产品描述：KAPA HiFi HotStart DNA Polymerase is an antibody-based hot start formulation of KAPA HiFi DNA Polymerase, a novel B-family DNA polymerase exhibiting industry-leading performance in comparison with other high-fidelity (B-family) DNA polymerases and polymerase blends. KAPA HiFi DNA Polymerase was engineered for increased affinity to DNA, without the need for accessory protein domains. The intrinsic high processivity of the enzyme results in significant improvements in yield, sensitivity, speed, target length and the ability to amplify difficult amplicons. These enhancements result in lower amplification bias which leads to more uniform sequence coverage. In the HotStart formulation, a proprietary antibody inactivates the polymerase until the first denaturation step. This eliminates spurious amplification products resulting from non-specific priming events during reaction setup and initiation, and increases overall reaction efficiency.

产品特点：

1· Real-time, high fidelity amplification of next-generation DNA sequencing libraries；Enzyme bias – along with other artifacts such as PCR-induced errors, adaptor dimers, PCR duplicates, and chimeras – is exacerbated by over-amplification, while under-amplification results in insufficient yields. Inherent uncertainty in the outcome of end-point PCR often demands downstream validation of library quality by electrophoresis. KAPA Real-Time PCR Library Amplification Kits provide a high fidelity, real-time PCR method for rapid and convenient amplification of libraries. Precise control over the number of cycles required for optimal amplification eliminates expensive and time-consuming post- enrichment gel electrophoresis

2·KAPA Real-Time PCR Library Amplification Kit workflow；

保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分

定购信息：

产品名称	规格	操作手册	货号	询价
高保真二代测序文库实时定量扩增试剂盒	50 rxn	PDF	A-KK2701
高保真二代测序文库实时定量扩增试剂盒	250 rxn	PDF	A-KK2702
Bio-Rad SYBR快速定量试剂盒	1 ml	PDF	A-KK4606
Bio-Rad SYBR快速定量试剂盒	5 ml	PDF	A-KK4607
Bio-Rad SYBR快速定量试剂盒	10 ml	PDF	A-KK4608
ABI Prism SYBR快速定量试剂盒	1 mL	PDF	A-KK4603
ABI Prism SYBR快速定量试剂盒	5 mL	PDF	A-KK4604
ABI Prism SYBR快速定量试剂盒	10 mL	PDF	A-KK4605
Paq5000 Hotstart PCR Master Mix	400 次	PDF	A-600872
Paq5000 Hotstart PCR Master Mix	100 次	PDF	A-600870
植物DNA小量提取试剂盒	50 次	PDF	A-DH813-01
植物DNA小量提取试剂盒	200 次	PDF	A-DH813-02
凝胶DNA纯化回收试剂盒	100 次	PDF	A-D2110-01
凝胶DNA纯化回收试剂盒	200 次	PDF	A-D2110-02
凝胶DNA纯化回收试剂盒	500 次	PDF	A-D2110-03
凝胶DNA纯化回收试剂盒	1000次	PDF	A-D2110-04
胶自动纯化回收试剂盒(磁珠法)	150 次	PDF	A-DM136-01
胶自动纯化回收试剂盒(磁珠法)	500 次	PDF	A-DM136-02
总RNA自动化富集纯化mRNA试剂盒(磁珠法)	30 次	PDF	A-RM911-01
植物自动化DNA大量提取试剂盒(磁珠法)	50 次	PDF	A-DM311-01
组织切片DNA极速提取试剂盒	100 次	PDF	A-P-1003-100

表观遗传学产品全面解决方案列下：

产品名称	规格	操作手册	货号	询价
DNA羟甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1037-48
DNA羟甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1037-96
DNA羟甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1036-48
DNA羟甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1036-96
DNA甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1035-48
DNA甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1035-96
DNA甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1034-48
DNA甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1034-96
通用DNA甲基化定量试剂盒	48 次	PDF	A-P-1011-48
通用DNA甲基化定量试剂盒	96 次	PDF	A-P-1011-96
超敏DNA甲基化定量试剂盒	48 次	PDF	A-P-1021-48
超敏DNA甲基化定量试剂盒	96 次	PDF	A-P-1021-96
总DNA甲基化定量试剂盒	48 次	PDF	A-P-1014-48
总DNA甲基化定量试剂盒	96 次	PDF	A-P-1014-96

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关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC

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时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶，并且区分5-hmC, 5-mC, 和 C，使得研究者能够重新评估他们的DNA甲基化信息，也能够在新样品中寻找DNA羟甲基化。