Roche 454 FLX-SYBR Fast Universal 货号：A-KK4820
Roche 454 FLX-SYBR Fast Universal
背景资料：Standard methods for quantifying next-generation sequencing libraries have a number of important disadvantages. Electrophoresis and spectrophotometry measure total nucleic acid concentrations, whereas optimal cluster density or template-to-bead ratio depend on the appropriate concentration of PCR-amplifiable DNA molecules. These methods also have low sensitivity, consuming nanograms of precious samples, and are not suitable for high-throughput workflows.
产品描述：KAPA Library Quantification Kits are optimized for the Illumina Genome Analyzer, Roche 454 Titanium series, and Roche 454 FLX series platforms and include highly reproducible and precisely defined sets of serially diluted DNA concentration standards and state-of-the-art qPCR reagents, which contain a DNA polymerase specifically engineered for robust, SYBR® Green I-tolerant amplification of long and difficult templates.
产品特点：
1· Reliable quantification results in consistent cluster density；
2·Reliable DNA concentration standards with minimal variability from lot-to-lot；
3·qPCR library quantification results in streamlined workflows；
4.Efficient amplification of a wide range of templates during qPCR.
保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分
定购信息：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| Roche 454 FLX-SYBR Fast Universal |
1 set |
PDF |
A-KK4820 |
 |
| Roche 454 FLX-SYBR Fast Universal |
1 set |
PDF |
A-KK4821 |
|
| Bio-Rad SYBR快速定量试剂盒 |
1 ml |
PDF |
A-KK4606 |
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| Bio-Rad SYBR快速定量试剂盒 |
5 ml |
PDF |
A-KK4607 |
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| Bio-Rad SYBR快速定量试剂盒 |
10 ml |
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A-KK4608 |
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| ABI Prism SYBR快速定量试剂盒 |
1 mL |
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A-KK4603 |
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| ABI Prism SYBR快速定量试剂盒 |
5 mL |
PDF |
A-KK4604 |
|
| ABI Prism SYBR快速定量试剂盒 |
10 mL |
PDF |
A-KK4605 |
|
| Paq5000 Hotstart PCR Master Mix |
400 次 |
PDF |
A-600872 |
|
| Paq5000 Hotstart PCR Master Mix |
100 次 |
PDF |
A-600870 |
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| 植物DNA小量提取试剂盒 |
50 次 |
PDF |
A-DH813-01 |
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| 植物DNA小量提取试剂盒 |
200 次 |
PDF |
A-DH813-02 |
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| 凝胶DNA纯化回收试剂盒 |
100 次 |
PDF |
A-D2110-01 |
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| 凝胶DNA纯化回收试剂盒 |
200 次 |
PDF |
A-D2110-02 |
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| 凝胶DNA纯化回收试剂盒 |
500 次 |
PDF |
A-D2110-03 |
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| 凝胶DNA纯化回收试剂盒 |
1000次 |
PDF |
A-D2110-04 |
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| 胶自动纯化回收试剂盒(磁珠法) |
150 次 |
PDF |
A-DM136-01 |
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500 次 |
PDF |
A-DM136-02 |
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30 次 |
PDF |
A-RM911-01 |
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| 植物自动化DNA大量提取试剂盒(磁珠法) |
50 次 |
PDF |
A-DM311-01 |
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| 组织切片DNA极速提取试剂盒 |
100 次 |
PDF |
A-P-1003-100 |
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表观遗传学产品全面解决方案列下：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| DNA羟甲基化定量试剂盒(荧光法) |
48 次 |
PDF |
A-P-1037-48 |
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| DNA羟甲基化定量试剂盒(荧光法) |
96 次 |
PDF |
A-P-1037-96 |
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| DNA羟甲基化定量试剂盒(比色法) |
48 次 |
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A-P-1036-48 |
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| DNA羟甲基化定量试剂盒(比色法) |
96 次 |
PDF |
A-P-1036-96 |
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| DNA甲基化定量试剂盒(荧光法) |
48 次 |
PDF |
A-P-1035-48 |
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| DNA甲基化定量试剂盒(荧光法) |
96 次 |
PDF |
A-P-1035-96 |
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| DNA甲基化定量试剂盒(比色法) |
48 次 |
PDF |
A-P-1034-48 |
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| DNA甲基化定量试剂盒(比色法) |
96 次 |
PDF |
A-P-1034-96 |
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48 次 |
PDF |
A-P-1011-48 |
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| 通用DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1011-96 |
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| 超敏DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1021-48 |
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| 超敏DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1021-96 |
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| 总DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1014-48 |
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| 总DNA甲基化定量试剂盒 |
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Accurate quantification of PCR-competent sequencing templates is crucial for reliable clonal amplification via either emulsion PCR (emPCR) or bridge PCR (bPCR) - underestimation results in non-clonality, while overestimation leads to inefficiency via poor yields of clonally amplified templates.
KAPA Library Quantification kits combine a quality-controlled set of DNA quantification standards with the unmatched performance of KAPA SYBR® FAST qPCR reagents to provide a rapid, sensitive, and reliable method for quantifying amplifiable molecules in next-generation sequencing DNA libraries.
All kits contain 5 mL KAPA SYBR® FAST qPCR Master Mix (2X), 1 mL Primer Premix, and 6 x 80 µL DNA Quantification Standards. Kits contain primers, DNA standards, and qPCR reagents specific for both DNA sequencing platform (Illumina GA, Roche 454 Titanium, Roche 454 FLX) and qPCR instrument (Universal, ABI Prism®, Bio-Rad iCycler™, and Roche LightCycler® 480). Primer Premix and DNA Quantification Standards are also sold separately |
