快速HRM PCR 试剂盒                                                                   货号：A-KK4201

KAPA HRM FAST PCR Kit

背景资料：The KAPA HRM FAST PCR Kit is a convenient, ready-to-use master mix designed for the high performance detection of DNA sequence variations by High Resolution Melt (HRM) analysis. The kit contains a novel DNA polymerase, engineered via a process of molecular evolution, for fast and efficient DNA amplification in the presence of high concentrations of intercalating fluorescent dyes. KAPA HRM FAST PCR Kits also contain EvaGreen®, a next-generation, saturating fluorescent dye which selectively binds to double-stranded DNA. In contrast to SYBR® Green I dye, EvaGreen® can be used at higher concentrations without PCR inhibition and shows equal binding affinity for GC-rich and AT-rich regions.

产品描述：The combination of an engineered DNA polymerase and EvaGreen® dye enables amplification and discrimination of even the most challenging sequence differences (i.e. Type IV) without sequence preference.

KAPA HRM FAST PCR Kits are optimized for endpoint HRM analysis and are not recommended for quantitative PCR. Kits are ideally suited for:

SNP genotyping

Mutation discovery

Species identification and genotyping

DNA fingerprinting

Genetic association studies

产品特点：

1.Accurate SNP genotyping with maximum sensitivity and speed.;KAPA HRM FAST PCR Kits are engineered to maximize differences in melting behavior between sequence variants. Kits contain a novel DNA polymerase and buffer system that allows for fast PCR cycling and improved sensitivity. Single nucleotide polymorphism (SNP) genotpying is the most common application for HRM. In this example, KAPA HRM FAST is compared to other HRM kits when genotyping Type I SNP rs12913832 (G/A).

2.Fast and reproducible detection of Type IV SNPs.;The Type IV SNP rs641805 is located on an intron of the dihydropyrimidine dehydrogenase (DPD) gene, which catalyzes the reduction of uracil and thymine and is associated with the degradation of certain chemotherapeutic drugs. Reactions (20 μl) contained 1X KAPA HRM FAST PCR Master Mix, 2.5 mM MgCl2, 0.2 μM of each primer (targeting a 124 bp amplicon surrounding the SNP of interest) and 10 ng of human genomic DNA (all three genotypes, confirmed by sequencing). PCR and HRM was performed with the Corbett Rotor-GeneTM 6000 instrument, using a fast, 2-step cycling protocol (40 cycles) with 5 sec denaturation (95 °C) and 20 sec annealing/extension (60 °C). The ABI MeltDoctor HRM Master Mix was used according to the manufacturer’s instructions, with the same template DNA samples. ABI MeltDoctorTM is unable to differentiate the Type IV SNP, while KAPA HRM FAST detects the SNP in 95% of the samples assayed

3.From cell to SNP: Rapid genotyping directly from buccal swabs.;The combination of the KAPA Express Extract DNA Extraction Kit (containing a novel thermostable protease) and the KAPA HRM FAST PCR Kit enables extraction, amplification and HRM in less than 2 hours. DNA extracts were prepared from buccal swabs obtained from 36 individuals using KAPA Express Extract DNA Extraction Kits (according to recommended protocols). DNA extracts (1 μl of each) were used directly in 20 μl KAPA HRM FAST reactions containing 1X KAPA HRM FAST Master Mix, 2.5 mM MgCl2 and 0.2 μM of each primer (targeting a 75 bp amplicon surrounding the SNP of interest). PCR and HRM was performed with the Corbett Rotor-GeneTM 6000 instrument, using a fast, 2-step cycling protocol (45 cycles) with 5 sec denaturation (95 °C) and 30 sec annealing/extension (60 °C).

保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分

定购信息：

产品名称	规格	操作手册	货号	询价
快速HRM PCR 试剂盒	1 ml	PDF	A-KK4201
快速HRM PCR 试剂盒	5 ml	PDF	A-KK4202
快速HRM PCR 试剂盒	2*5ml	PDF	A-KK4203
ABI SOLiD-SYBR Fast Roche Light Cycler480	1 set	PDF	A-KK4853
Bio-Rad SYBR快速定量试剂盒	1 ml	PDF	A-KK4606
Bio-Rad SYBR快速定量试剂盒	5 ml	PDF	A-KK4607
Bio-Rad SYBR快速定量试剂盒	10 ml	PDF	A-KK4608
ABI Prism SYBR快速定量试剂盒	1 mL	PDF	A-KK4603
ABI Prism SYBR快速定量试剂盒	5 mL	PDF	A-KK4604
ABI Prism SYBR快速定量试剂盒	10 mL	PDF	A-KK4605
Paq5000 Hotstart PCR Master Mix	400 次	PDF	A-600872
Paq5000 Hotstart PCR Master Mix	100 次	PDF	A-600870
植物DNA小量提取试剂盒	50 次	PDF	A-DH813-01
植物DNA小量提取试剂盒	200 次	PDF	A-DH813-02
凝胶DNA纯化回收试剂盒	100 次	PDF	A-D2110-01
凝胶DNA纯化回收试剂盒	200 次	PDF	A-D2110-02
凝胶DNA纯化回收试剂盒	500 次	PDF	A-D2110-03
凝胶DNA纯化回收试剂盒	1000次	PDF	A-D2110-04
胶自动纯化回收试剂盒(磁珠法)	150 次	PDF	A-DM136-01
胶自动纯化回收试剂盒(磁珠法)	500 次	PDF	A-DM136-02
总RNA自动化富集纯化mRNA试剂盒(磁珠法)	30 次	PDF	A-RM911-01
植物自动化DNA大量提取试剂盒(磁珠法)	50 次	PDF	A-DM311-01
组织切片DNA极速提取试剂盒	100 次	PDF	A-P-1003-100

表观遗传学产品全面解决方案列下：

产品名称	规格	操作手册	货号	询价
DNA羟甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1037-48
DNA羟甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1037-96
DNA羟甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1036-48
DNA羟甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1036-96
DNA甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1035-48
DNA甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1035-96
DNA甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1034-48
DNA甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1034-96
通用DNA甲基化定量试剂盒	48 次	PDF	A-P-1011-48
通用DNA甲基化定量试剂盒	96 次	PDF	A-P-1011-96
超敏DNA甲基化定量试剂盒	48 次	PDF	A-P-1021-48
超敏DNA甲基化定量试剂盒	96 次	PDF	A-P-1021-96
总DNA甲基化定量试剂盒	48 次	PDF	A-P-1014-48
总DNA甲基化定量试剂盒	96 次	PDF	A-P-1014-96

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时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶，并且区分5-hmC, 5-mC, 和 C，使得研究者能够重新评估他们的DNA甲基化信息，也能够在新样品中寻找DNA羟甲基化。