体内总蛋白SUMO化修饰检测试剂盒                                                                         货号：A-P-8001

EpiQuik™ In Vivo Protein Sumoylation Assay Ultra Kit 

背景资料：SUMOylation is a post-translational modification involved in various cellular processes, such as nuclear-cytosolic transport, transcriptional regulation, apoptosis, protein stability, response to stress, and progression through the cell cycle. SUMO proteins are similar to ubiquitin. There are 3 confirmed SUMO isoforms in humans: SUMO-1, SUMO-2, and SUMO-3. SUMO-2/3 show a high degree of similarity to each other and are distinct from SUMO-1. SUMOylation is directed by an enzymatic cascade analogous to that involved in ubiquitination. SUMOylation of target proteins in vivo has been shown to cause a number of different outcomes, including altered localization and binding partners. Detection of in vivo protein SUMOylation (SUMO conjugation) would provide useful information for understanding SUMO modification that emerges as an important control mechanism regulating the activity of many nuclear proteins。

产品描述：In an assay with this kit, the antibodies specific to the targeted proteins are stably bound to the strip wells and the targeted proteins are captured by these antibodies. SUMOylation of the targeted proteins are detected by recognition of SUMO conjugated to these proteins with an anti-SUMO antibody. The ratio or intensity of the SUMOylation, which is proportional to the conjugated SUMO amount, can be quantified through the signal report-color development system by using a microplate reader at 450 nm wavelength；The EpiQuik™ In Vivo Protein Sumoylation Assay Ultra Kit is a complete set of optimized reagents designed for measuring in vivo protein SUMOylation from multiple mammalian cells/tissues including human, mouse, and rat 。  

产品特点：

1·Fast procedure, which can be finished within 5 hours；操作十分快捷，5小时内可完成；

2·Direct colorimetric assay without the need for affinity chromatography or Western blot；利用一步比色度检测方法，不接触放射性物质，无需进行色谱分析和Western blotting；

3·Flexible choice of antibody of interest allows for the detection of SUMOylation of multiple target proteins simultaneously；柔性抗体可以同时检测发生类小泛素修饰作用的各种靶蛋白；

4·Use of optimized detection antibody eliminates the step for detection solution preparation, thereby increasing detection sensitivity and assay convenience；

5.The positive control (SUMO protein) and negative control (un-SUMOylated non-immune IgG protein) allows protein SUMOylation to be quantified；附有SUMO蛋白作为阳性对照和非SUMO蛋白non-immune IgG protein，来定量发生了类小泛素修饰作用的蛋白；

6.Strip microplate format makes the assay flexible: manual or high throughput analysis；96孔板模式使研究人员能根据自己需要选择手工或是高通量分析；

7·Reliable and consistent assay conditions；操作简便、结果可靠、统一的分析条件。

保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分

定购信息：

产品名称	规格	操作手册	货号	询价
体内总蛋白SUMO化超敏分析检测试剂盒（比色法）	48 次	PDF	A-P-8003-48
体内总蛋白SUMO化超敏分析检测试剂盒（比色法）	96 次	PDF	A-P-8003-96
体内总蛋白SUMO化修饰检测试剂盒	48 次	PDF	A-P-8001-48
体内总蛋白SUMO化修饰检测试剂盒	96 次	PDF	A-P-8001-96

表观遗传学产品全面解决方案列下：

产品名称	规格	操作手册	货号	询价
DNA羟甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1037-48
DNA羟甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1037-96
DNA羟甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1036-48
DNA羟甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1036-96
DNA甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1035-48
DNA甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1035-96
DNA甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1034-48
DNA甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1034-96
通用DNA甲基化试剂盒(定性分析)	48 次	PDF	A-P-1011-48
通用DNA甲基化试剂盒(定性分析)	96 次	PDF	A-P-1011-96
超敏DNA甲基化定量试剂盒	48 次	PDF	A-P-1021-48
超敏DNA甲基化定量试剂盒	96 次	PDF	A-P-1021-96
总DNA甲基化定量试剂盒	48 次	PDF	A-P-1014-48
总DNA甲基化定量试剂盒	96 次	PDF	A-P-1014-96

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