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043.Abbkine精品推荐------DyLight 405蓝色荧光二抗!

DyLigh™系列荧光标记是一种近年来被广泛应用的新型荧光染料,由于具有很好的光谱宽度、更强的荧光强度,更高的光学耐受性(抗淬灭性)、对pH不敏感、分子较小而渗透性 更好的优势,越来越受到科研客户的喜爱。DyLight™种类齐全,如Dylight 405是蓝色荧光,替代Alexa 405;DyLight 405是绿色荧光,可完全替代FITC/Cy2/Alexa 488;完全替代Cy3/Rhodamin/Alexa 546/Alexa 555的Dylight 549;替代Texas Red/Alexa 594的Dylight 594;Dylight 633和Dylight 649(可替代Cy5/Alexa 647)则是更强的红色荧光;另外,Dylight还有适配Odyssey系统的近红外和远红外二抗Dylight 680和DyLight 800。

 

 

荧光三标图示—Dylight 405用于荧光三标
   
荧光四标图示—Dylight 405用于荧光四标 
图 1(荧光三标图示):小鼠视网膜组织使用兔源GFAP一抗和DyLight 405抗兔IgG(假绿色)标记,同时DyLight 405抗小鼠IgG(假红色)配合小鼠源neurofilament抗体标记神经丝,Cy3驴抗鸡IgG(假蓝色)与鸡源collagen IV抗体标记出蓝色的胶原蛋白IV。以N-没食子酸丙酯-甘油封片,并以配有405nm激光和氪/氩激光的Olympus Flouview 1000激光扫描共聚焦显微镜拍摄。     图 2(荧光四标图示):小鼠视网膜组织使用兔源GFAP一抗和DyLight 405抗兔IgG(假绿色)标记,同时DyLight 405抗小鼠IgG(假红色)配合小鼠源neurofilament抗体标记神经丝,Cy3驴抗鸡IgG(假蓝色)与鸡源vimentin抗体标记出蓝色的波形蛋白,DyLight 649抗山羊二抗与山羊源collagen IV抗体标记胶原蛋白IV。以N-没食子酸丙酯-甘油封片,并以配有405nm激光和氪/氩激光的Olympus Flouview 1000激光扫描共聚焦显微镜拍摄。
替代文本
   
图 3:使用 FACSCalibur流式细胞仪,分析抗-CD3和其他荧光素标记的人外周血淋巴细胞图谱。DyLight 405 (绿色)、FITC (黄色)、Cy2 (蓝色)标记的二抗都来自Jackson公司。另外Alexa Fluor 488购自Invitrogen。箭头所指处显示Alexa Fluor 488在这个实验中有轻微的背景。    

图 4:以时间为横坐标,初始荧光强度的百分比为纵坐标绘制的图谱,HEp-2细胞以ANA(Bio-Rad)和各种山羊抗人IgG(H+L)的二抗标记, 细胞用不含抗淬灭剂的PBS-90%甘油封片。显示DyLight 405和Alexa 488均有较好的光学稳定性,FITC最容易淬灭,Cy2次之。

 

 

DyLight 405标记的抗体比传统的蓝色Cascade Blue标记的更亮,与Alexa Fluor 405 标记的亮度相当,但背景更低;从光稳定性上来说,DyLight 405与Alexa 405均表现优异,均强于Cascade Blue。另外,DyLight荧光标记的二抗比传统的荧光标记以及Alexa都具有更高的性价比。另外,蓝色的DyLight 405可以配合绿色DyLight 488、DyLight 549(或DyLight 594)等做出三标荧光实验,更能与DyLight 405、DyLight 549(或DyLight 594)、DyLight 649做出四标荧光实验。

 

我们向您推荐美国Abbkine原装进口的液体形式的小包装常用DyLight系列二抗产品,以及Jackson各种规格的全系列DyLight二抗产品。 Abbkine是美国专业生产免疫试剂并提供细胞学研究解决方案的公司,其产品主要销往到全球的的大学、科研院所等研究机构。其中Abbkine为您提供 最常用的DyLight 405荧光标记二抗产品:

 

 

品名 品牌 货号 规格
DyLight 405 AffiniPure Goat Anti-Mouse IgG(H+L) Abbkine A23110 100μl/500μl
DyLight 405 AffiniPure Goat Anti-Rabbit IgG(H+L) Abbkine A23120 100μl/500μl
DyLight 405 AffiniPure Rabbit Anti-Goat IgG(H+L) Abbkine A23130 100μl/500μl
DyLight 405 AffiniPure Goat Anti-Rat IgG(H+L) Abbkine A23140 100μl/500μl

 

 

Jackson 是全球专业从事生产和销售亲和纯化的二抗和纯化免疫球蛋白的公司,其抗体产品线完全,有全球最大的二抗产品库,能提供多种应用类型和需要的二抗产品,其二 抗产品本身也具有极高的产品质量和稳定性,其产品主要销往到世界各地的大学科研所和医院里的动物研究以及生物医学研究机构的科学家。Jackson能为您 提供最全的DyLight 405荧光标记二抗:

 

1、Jackson DyLight 405抗小鼠IgG二抗产品如下:

 

 

品名 品牌 货号 规格
DyLight 405 AffiniPure Donkey Anti-Mouse IgG (H+L) (min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Rb, Shp Sr Prot) Jackson 715-475-150 0.5mg
DyLight 405 AffiniPure Donkey Anti-Mouse IgG (H+L) (min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Rb, Rat, Shp Sr Prot) Jackson 715-475-151 0.5mg
DyLight 405 AffiniPure Donkey Anti-Mouse IgM, µ chain specific Jackson 715-475-020 2.0mg
DyLight 405 AffiniPure Donkey Anti-Mouse IgM, µ chain specific (min X Hu, Bov, Hrs, Rat Sr Prot) Jackson 715-475-140 0.5mg
DyLight 405 AffiniPure Goat Anti-Mouse IgG (H+L) Jackson 115-475-003 2.0mg
DyLight 405 AffiniPure Goat Anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) Jackson 115-475-062 1.5mg
DyLight 405 AffiniPure Goat Anti-Mouse IgG (H+L)  (min X Hu, Bov, Hrs, Rb, Sw Sr Prot) Jackson 115-475-146 1.5mg
DyLight 405 AffiniPure Goat Anti-Mouse IgG (H+L) (min X Rat, Hu, Bov, Hrs, Rb, Sr Prot) Jackson 115-475-166 0.5mg
DyLight 405 AffiniPure Goat Anti-Mouse IgG,  Light Chain Specific for Western blotting (min X Bov, Gt, Hrs, Hu, Rb, Rat, Shp Ig) Jackson 115-475-174 0.5mg
DyLight 405 AffiniPure Goat Anti-Mouse IgG, Fcγ fragment specific Jackson 115-475-008 2.0mg
DyLight 405 AffiniPure Goat Anti-Mouse IgG, Fcγ fragment specific (min X Hu, Bov, Hrs Sr Prot) Jackson 115-475-071 1.5mg
DyLight 405 AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 1 specific (min X Hu, Bov, Rb Sr Prot) Jackson 115-475-205 0.5mg
DyLight 405 AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2a specific (min X  Hu, Bov, Rb Sr Prot) Jackson 115-475-206 0.5mg
DyLight 405 AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2b specific (min X  Hu, Bov, Rb Sr Prot) Jackson 115-475-207 0.5mg
DyLight 405 AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2c specific (min X  Hu, Bov, Rb Sr Prot) Jackson 115-475-208 0.5mg
DyLight 405 AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 3 specific (min X  Hu, Bov, Rb Sr Prot) Jackson 115-475-209 0.5mg
DyLight 405 AffiniPure Goat Anti-Mouse IgG (subclasses 1+2a+2b+3), Fcγ fragment specific (min X Hu,Bov, Rb Sr Prot) Jackson 115-475-164 1.0mg
DyLight 405 AffiniPure Goat Anti-Mouse IgG, F(ab')2 fragment specific Jackson 115-475-006 2.0mg
DyLight 405 AffiniPure Goat Anti-Mouse IgG, F(ab')2 fragment specific (min X Hu, Bov, Hrs Sr Prot) Jackson 115-475-072 1.5mg
DyLight 405 AffiniPure Goat Anti-Mouse IgG + IgM (H+L) Jackson 115-475-044 2.0mg
DyLight 405 AffiniPure Goat Anti-Mouse IgG + IgM(H+L) (min X Hu, Bov,Hrs Sr Prot) Jackson 115-475-068 1.5mg
DyLight 405 AffiniPure Goat Anti-Mouse IgM, µ chain specific Jackson 115-475-020 2.0mg
DyLight 405 AffiniPure Goat Anti-Mouse IgM, µ chain specific  (min X Hu, Bov, Hrs Sr Prot) Jackson 115-475-075 1.0mg
DyLight 405 AffiniPure Rabbit Anti-Mouse IgG (H+L) Jackson 315-475-003 1.5mg
DyLight 405 AffiniPure Rabbit Anti-Mouse IgG (H+L) (min X Hu Sr Prot) Jackson 315-475-045 1.0mg
DyLight 405 AffiniPure Rabbit Anti-Mouse IgG, Fcγ fragment specific Jackson 315-475-008 1.5mg
DyLight 405 AffiniPure Rabbit Anti-Mouse IgG, Fcγ fragment specific (min X Hu Sr Prot) Jackson 315-475-046 1.0mg
DyLight 405 AffiniPure Rabbit Anti-Mouse IgG, F(ab')2 fragment specific Jackson 315-475-006 1.5mg
DyLight 405 AffiniPure Rabbit Anti-Mouse IgG, F(ab')2 fragment specific (min X Hu Sr Prot) Jackson 315-475-047 1.0mg
DyLight 405 AffiniPure Rabbit Anti-Mouse IgG + IgM (H+L) Jackson 315-475-044 1.5mg
DyLight 405 AffiniPure Rabbit Anti-Mouse IgG + IgM (H+L) (min X Hu Sr Prot) Jackson 315-475-048 1.0mg
DyLight 405 AffiniPure Rabbit Anti-Mouse IgM, µ chain specific Jackson 315-475-020 1.5mg
DyLight 405 AffiniPure Rabbit Anti-Mouse IgM, µ chain specific (min X Hu Sr Prot) Jackson 315-475-049 1.0mg
DyLight 405 AffiniPure Rat Anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs, Rat, Rb Sr Prot) Jackson 415-475-166 1.0mg
DyLight 405 AffiniPure Sheep Anti-Mouse IgG (H+L) Jackson 515-475-003 2.0mg
DyLight 405 AffiniPure Sheep Anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) Jackson 515-475-062 1.5mg
DyLight 405 AffiniPure Sheep Anti-Mouse IgG, Fcγ fragment specific (min X Hu, Bov, Hrs Sr Prot) Jackson 515-475-071 1.5mg
DyLight 405 AffiniPure Sheep Anti-Mouse IgG, F(ab')2 fragment specific (min X Hu, Bov, Hrs Sr Prot) Jackson 515-475-072 1.5mg

 

 

2、Jackson DyLight 405抗兔IgG二抗产品如下:

 

 

品名 品牌 货号 规格
DyLight 405 AffiniPure Donkey Anti-Rabbit IgG (H+L) (min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Ms, Rat, Shp Sr Prot) Jackson 711-475-152 0.5mg
DyLight 405 AffiniPure Goat Anti-Rabbit IgG (H+L) Jackson 111-475-003 2.0mg
DyLight 405 AffiniPure Goat Anti-Rabbit IgG (H+L) (min X Hu Sr Prot) Jackson 111-475-045 1.5mg
DyLight 405 AffiniPure Goat Anti-Rabbit IgG (H+L) (min X Hu, Ms, Rat Sr Prot) Jackson 111-475-144 1.5mg
DyLight 405 AffiniPure IgG Fraction Monoclonal Mouse Anti-Rabbit IgG, Light Chain Specific for Western blotting (min X Bov, Gt, Ar Hms, Hrs, Hu, Ms, Rat, Shp Ig) Jackson 211-472-171 0.5mg
DyLight 405 AffiniPure Goat Anti-Rabbit IgG, Fc fragment specific Jackson 111-475-008 2.0mg
DyLight 405 AffiniPure Goat Anti-Rabbit IgG, Fc fragment specific (min X Hu Sr Prot) Jackson 111-475-046 1.5mg
DyLight 405 AffiniPure Goat Anti-Rabbit IgG, F(ab')2 fragment specific Jackson 111-475-006 2.0mg
DyLight 405 AffiniPure Goat Anti-Rabbit IgG, F(ab')2 fragment specific (min X Hu Sr Prot) Jackson 111-475-047 1.5mg
DyLight 405 AffiniPure Polyclonal Mouse Anti-Rabbit IgG (H+L) (min X Hu, Gt, Ms, Shp Sr Prot) Jackson 211-475-109 1.0mg

 

 

3、Jackson DyLight 405抗山羊二抗产品如下:

[

 

品名 品牌 货号 规格
DyLight 405 AffiniPure Bovine Anti-Goat IgG (H+L) (min X Bov, Ck, GP, Sy Hms, Hrs, Hu, Ms, Rb, Rat Sr Prot) Jackson 805-475-180 0.5mg
DyLight 405 AffiniPure Donkey Anti-Goat IgG (H+L) Jackson 705-475-003 1.0mg
DyLight 405 AffiniPure Donkey Anti-Goat IgG (H+L) (min X Ck, GP, Sy Hms, Hrs, Hu, Ms, Rb, Rat Sr Prot) Jackson 705-475-147 0.5mg
DyLight 405 AffiniPure Mouse Anti-Goat IgG (H+L) (min X Ms, Hu, Rb Sr Prot) Jackson 205-475-108 1.0mg
DyLight 405 AffiniPure Rabbit Anti-Goat IgG (H+L) Jackson 305-475-003 1.5mg
DyLight 405 AffiniPure Rabbit Anti-Goat IgG (H+L) (min X Hu Sr Prot) Jackson 305-475-045 1.0mg
DyLight 405 AffiniPure Rabbit Anti-Goat IgG, Fc fragment specific Jackson 305-475-008 1.5mg
DyLight 405 AffiniPure Rabbit Anti-Goat IgG, Fc fragment specific (min X Hu Sr Prot) Jackson 305-475-046 1.0mg
DyLight 405 AffiniPure Rabbit Anti-Goat IgG, F(ab')2 fragment specific Jackson 305-475-006 1.5mg
DyLight 405 AffiniPure Rabbit Anti-Goat IgG, F(ab')2 fragment specific (min X Hu Sr Prot) Jackson 305-475-047 1.0mg

 

 

4、Jackson DyLight 405抗大鼠二抗产品如下:

 

 

品名 品牌 货号 规格
DyLight 405 AffiniPure Donkey Anti-Rat IgG (H+L)  (min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Rb, Shp Sr Prot) Jackson 712-475-150 0.5mg
DyLight 405 AffiniPure Donkey Anti-Rat IgG (H+L) (min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Ms, Rb, Shp Sr Prot) Jackson 712-475-153 0.5mg
DyLight 405 AffiniPure Goat Anti-Rat IgG (H+L) Jackson 112-475-003 2.0mg
DyLight 405 AffiniPure Goat Anti-Rat IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) Jackson 112-475-062 1.5mg
DyLight 405 AffiniPure Goat Anti-Rat IgG (H+L) (min X Hu, Bov, Hrs, Rb Sr Prot) Jackson 112-475-143 1.5mg
DyLight 405 AffiniPure Goat Anti-Rat IgG (H+L) (min X Ms, Hu, Bov, Hrs, Rb Sr Prot) Jackson 112-475-167 0.5mg
DyLight 405 AffiniPure Goat Anti-Rat IgG, Light Chain Specific for Western blotting (min X Bov, Gt, Hrs, Hu, Ms, Rat, Shp Ig) Jackson 112-475-175 0.5mg
DyLight 405 AffiniPure Goat Anti-Rat IgG, Fcγ fragment specific Jackson 112-475-008 2.0mg
DyLight 405 AffiniPure Goat Anti-Rat IgG, Fcγ fragment specific (min X Hu, Bov, Hrs Sr Prot) Jackson 112-475-071 1.5mg
DyLight 405 AffiniPure Goat Anti-Rat IgG, F(ab')2 fragment specific Jackson 112-475-006 2.0mg
DyLight 405 AffiniPure Goat Anti-Rat IgG, F(ab')2 fragment specific (min X Hu, Bov, Hrs Sr Prot) Jackson 112-475-072 1.5mg
DyLight 405 AffiniPure Goat Anti-Rat IgG + IgM (H+L) Jackson 112-475-044 2.0mg
DyLight 405 AffiniPure Goat Anti-Rat IgG + IgM (H+L) (min X Hu, Bov, Hrs Sr Prot) Jackson 112-475-068 1.5mg
DyLight 405 AffiniPure Goat Anti-Rat IgM, µ chain specific Jackson 112-475-020 2.0mg
DyLight 405 AffiniPure Goat Anti-Rat IgM, µ chain specific (min X Hu, Bov, Hrs Sr Prot) Jackson 112-475-075 1.0mg
DyLight 405 AffiniPure Mouse Anti-Rat IgG (H+L) (min X Ms Sr Prot) Jackson 212-475-082 1.5mg
DyLight 405 AffiniPure Mouse Anti-Rat IgG (H+L) (min X Hu, Bov, Hrs, Ms, Gt, Rb Sr Prot) Jackson 212-475-168 1.0mg
DyLight 405 AffiniPure Mouse Anti-Rat IgG, Fcγ fragment specific (min X Hu, Bov, Hrs, Ms Sr Prot) Jackson 212-475-104 1.0mg
DyLight 405 AffiniPure Mouse Anti-Rat IgG, F(ab')2 fragment specific (min X Hu, Bov, Hrs, Ms Sr Prot) Jackson 212-475-106 1.0mg
DyLight 405 AffiniPure Rabbit Anti-Rat IgG (H+L) Jackson 312-475-003 1.5mg
DyLight 405 AffiniPure Rabbit Anti-Rat IgG (H+L) (min X Hu Sr Prot) Jackson 312-475-045 1.0mg
DyLight 405 AffiniPure Rabbit Anti-Rat IgG, Fcγ fragment specific (min X Hu Sr Prot) Jackson 312-475-046 1.0mg
DyLight 405 AffiniPure Rabbit Anti-Rat IgG, F(ab')2 fragment specific (min X Hu Sr Prot) Jackson 312-475-047 1.0mg
DyLight 405 AffiniPure Rabbit Anti-Rat IgG + IgM (H+L) Jackson 312-475-044 1.5mg
DyLight 405 AffiniPure Rabbit Anti-Rat IgG + IgM (H+L) (min X Hu Sr Prot) Jackson 312-475-048 1.0mg
DyLight 405 AffiniPure Rabbit Anti-Rat IgM, µ chain specific Jackson 312-475-020 1.5mg
DyLight 405 AffiniPure Rabbit Anti-Rat IgM, µ chain specific (min X Hu Sr Prot) Jackson 312-475-049 1.0mg

 

 

5、Jackson DyLight 405抗人二抗产品如下:

 

 

品名 品牌 货号 规格
DyLight 405 AffiniPure Donkey Anti-Human IgG (H+L) (min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Ms, Rb, Rat, Shp Sr Prot) Jackson 709-475-149 0.5mg
DyLight 405 AffiniPure Donkey Anti-Human IgG, Fcγ fragment specific (min X Bov, Hrs, Ms Sr Prot) Jackson 709-475-098 1.0mg
DyLight 405 AffiniPure Donkey Anti-Human IgM, Fc5µ fragment specific (min X Bov, Hrs Sr Prot) Jackson 709-475-073 1.5mg
DyLight 405 AffiniPure Goat Anti-Human IgG (H+L) Jackson 109-475-003 2.0mg
DyLight 405 AffiniPure Goat Anti-Human IgG (H+L) (min X Bov, Hrs, Ms Sr Prot) Jackson 109-475-088 1.5mg
DyLight 405 AffiniPure Goat Anti-Human IgG, Fcγ   fragment specific Jackson 109-475-008 2.0mg
DyLight 405 AffiniPure Goat Anti-Human IgG, Fcγ fragment specific (min X Bov, Hrs, Ms Sr Prot) Jackson 109-475-098 1.0mg
DyLight 405 AffiniPure Goat Anti-Human IgG, F(ab')2 fragment specific Jackson 109-475-006 2.0mg
DyLight 405 AffiniPure Goat Anti-Human IgG, F(ab')2 fragment specific (min X Bov, Hrs, Ms Sr Prot) Jackson 109-475-097 1.5mg
DyLight 405 AffiniPure Goat Anti-Human IgG + IgM (H+L) Jackson 109-475-044 2.0mg
DyLight 405 AffiniPure Goat Anti-Human IgG + IgM (H+L) (min X Bov Sr Prot) Jackson 109-475-127 1.5mg
DyLight 405 AffiniPure Goat Anti-Human IgA + IgG + IgM (H+L) Jackson 109-475-064 2.0mg
DyLight 405 AffiniPure Goat Anti-Human IgM, Fc5µ fragment specific Jackson 109-475-043 2.0mg
DyLight 405 AffiniPure Goat Anti-Human IgM, Fc5µ fragment specific (min X Bov Sr Prot) Jackson 109-475-129 1.5mg
DyLight 405 AffiniPure Goat Anti-Human Serum IgA, α chain specific Jackson 109-475-011 2.0mg
DyLight 405 AffiniPure Mouse Anti-Human IgG (H+L) (min X Ms Sr Prot) Jackson 209-475-082 1.5mg
DyLight 405 AffiniPure Mouse Anti-Human IgG (H+L) (min X Bov, Hrs, Ms Sr  Prot) Jackson 209-475-088 1.0mg
DyLight 405 AffiniPure Mouse Anti-Human IgG, Fcγ fragment specific (min X Bov, Hrs, Ms Sr Prot) Jackson 209-475-098 1.0mg
DyLight 405 AffiniPure Mouse Anti-Human IgG, F(ab')2 fragment specific (min X Bov, Hrs, Ms Sr Prot) Jackson 209-475-097 1.0mg
 
 
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