| DyLigh™系列荧 光标记是一种近年来被广泛应用的新型荧光染料，由于具有很好的光谱宽度、更强的荧光强度，更高的光学耐受性（抗淬灭性）、对pH不敏感、分子较小而渗透性 更好的优势，越来越受到科研客户的喜爱。DyLight™种类齐全，如Dylight 405是蓝色荧光，替代Alexa 405；DyLight 549是绿色荧光，可完全替代FITC/Cy2/Alexa 488；完全替代Cy3/Rhodamin/Alexa 546/Alexa 555的Dylight 549；替代Texas Red/Alexa 594的Dylight 594；Dylight 633和Dylight 649（可替代Cy5/Alexa 647）则是更强的红色荧光；另外，Dylight还有适配Odyssey系统的近红外和远红外二抗Dylight 680和DyLight 800。
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图 1：HEp-2细胞使用ANA一抗（Bio-Rad）与亮度相当的荧光二抗标记。荧光显微镜下，上排图片的细胞用ProLong Gold (Invitrogen)抗淬灭剂封片，下排细胞用VECTASHIELD（Vector Labs）抗淬灭剂封片。Nikon LABOPHOT荧光显微镜连接D-70 DSLR 摄像机，100×曝光1秒拍摄的图片。
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图2、 以时间为横坐标，起始荧光强度的百分比为纵坐标绘制的图谱，HEp-2细胞以ANA（Bio-Rad）和Cy3/DyLight 549/Alexa 555山羊抗人IgG（H+L）的荧光二抗标记，细胞用不含抗淬灭剂的PBS-90%甘油封片。
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图3、 以时间为横坐标，初始荧光强度的百分比为纵坐标绘制的图谱，HEp-2细胞以ANA（Bio-Rad）和Cy3/DyLight 549/Alexa 555山羊抗人IgG（H+L）的荧光二抗标记，细胞用ProLong Gold封片，其他抗淬灭封片剂得到相似结果。
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DyLight 549标记的抗体比传统的罗丹明、TRITC等荧光亮，与Cy3 和Alexa Fluor 555亮度相当。对不含抗淬灭剂的PBS-90%甘油处理的封片进行荧光标记，DyLight 549与Alexa 555具有相当的耐光性，稍微比Cy3强。在封片剂中加入抗淬灭剂如ProLong Gold、VECTASHIELD或含N-propyl gallate的PBS-甘油等可增加这三种荧光染料的耐光性。在可见光谱的橙-红区域内，Jackson公司的DyLight 549和Cy3标记的二抗在所有免疫荧光实验中表现出最大灵敏度。
 我们向您推荐美国Abbkine原装进口的液体形式的小包装常用DyLight系列二抗产品，以及Jackson各种规格的全系列DyLight二抗产品。 Abbkine是美国专业生产免疫试剂并提供细胞学研究解决方案的公司，其产品主要销往到全球的的大学、科研院所等研究机构。其中Abbkine为您提供 最常用的DyLight 549荧光标记二抗产品：
| 品名 |
品牌 |
货号 |
规格 |
| DyLight 549 AffiniPure Goat Anti-Mouse IgG(H+L) |
Abbkine |
A23310 |
100μl/500μl |
| DyLight 549 AffiniPure Goat Anti-Rabbit IgG(H+L) |
Abbkine |
A23320 |
100μl/500μl |
| DyLight 549 AffiniPure Rabbit Anti-Goat IgG(H+L) |
Abbkine |
A23330 |
100μl/500μl |
| DyLight 549 AffiniPure Goat Anti-Rat IgG(H+L) |
Abbkine |
A23340 |
100μl/500μl |
 Jackson 是全球专业从事生产和销售亲和纯化的二抗和纯化免疫球蛋白的公司，其抗体产品线完全，有全球最大的二抗产品库，能提供多种应用类型和需要的二抗产品，其二 抗产品本身也具有极高的产品质量和稳定性，其产品主要销往到世界各地的大学科研所和医院里的动物研究以及生物医学研究机构的科学家。Jackson能为您 提供最全的DyLight 549荧光标记二抗：
1、Jackson DyLight 549抗小鼠IgG二抗产品如下：
| 品名 |
品牌 |
货号 |
规格 |
| DyLight 549 AffiniPure Donkey Anti-Mouse IgG (H+L) (min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Rb, Shp Sr Prot) |
Jackson |
715-505-150 |
0.5mg |
| DyLight 549 AffiniPure Donkey Anti-Mouse IgG (H+L) (min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Rb, Rat, Shp Sr Prot) |
Jackson |
715-505-151 |
0.5mg |
| DyLight 549 AffiniPure Donkey Anti-Mouse IgM, µ chain specific |
Jackson |
715-505-020 |
2.0mg |
| DyLight 549 AffiniPure Donkey Anti-Mouse IgM, µ chain specific (min X Hu, Bov, Hrs, Rat Sr Prot) |
Jackson |
715-505-140 |
0.5mg |
| DyLight 549 AffiniPure Goat Anti-Mouse IgG (H+L) |
Jackson |
115-505-003 |
2.0mg |
| DyLight 549 AffiniPure Goat Anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) |
Jackson |
115-505-062 |
1.5mg |
| DyLight 549 AffiniPure Goat Anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs, Rb, Sw Sr Prot) |
Jackson |
115-505-146 |
1.5mg |
| DyLight 549 AffiniPure Goat Anti-Mouse IgG (H+L) (min X Rat, Hu, Bov, Hrs, Rb, Sr Prot) |
Jackson |
115-505-166 |
0.5mg |
| DyLight 549 AffiniPure Goat Anti-Mouse IgG, Light Chain Specific for Western blotting (min X Bov, Gt, Hrs, Hu, Rb, Rat, Shp Ig) |
Jackson |
115-505-174 |
0.5mg |
| DyLight 549 AffiniPure Goat Anti-Mouse IgG, Fcγ fragment specific |
Jackson |
115-505-008 |
2.0mg |
| DyLight 549 AffiniPure Goat Anti-Mouse IgG, Fcγ fragment specific (min X Hu, Bov, Hrs Sr Prot) |
Jackson |
115-505-071 |
1.5mg |
| DyLight 549 AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 1 specific (min X Hu, Bov, Rb Sr Prot) |
Jackson |
115-505-205 |
0.5mg |
| DyLight 549 AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2a specific (min X Hu, Bov, Rb Sr Prot) |
Jackson |
115-505-206 |
0.5mg |
| DyLight 549 AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2b specific (min X Hu, Bov, Rb Sr Prot) |
Jackson |
115-505-207 |
0.5mg |
| DyLight 549 AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2c specific (min X Hu, Bov, Rb Sr Prot) |
Jackson |
115-505-208 |
0.5mg |
| DyLight 549 AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 3 specific (min X Hu, Bov, Rb Sr Prot) |
Jackson |
115-505-209 |
0.5mg |
| DyLight 549 AffiniPure Goat Anti-Mouse IgG (subclasses 1+2a+2b+3), Fcγ fragment specific (min X Hu,Bov, Rb Sr Prot) |
Jackson |
115-505-164 |
1.0mg |
| DyLight 549 AffiniPure Goat Anti-Mouse IgG, F(ab')2 fragment specific |
Jackson |
115-505-006 |
2.0mg |
| DyLight 549 AffiniPure Goat Anti-Mouse IgG, F(ab')2 fragment specific (min X Hu, Bov, Hrs Sr Prot) |
Jackson |
115-505-072 |
1.5mg |
| DyLight 549 AffiniPure Goat Anti-Mouse IgG + IgM (H+L) |
Jackson |
115-505-044 |
2.0mg |
| DyLight 549 AffiniPure Goat Anti-Mouse IgG + IgM(H+L) (min X Hu, Bov,Hrs Sr Prot) |
Jackson |
115-505-068 |
1.5mg |
| DyLight 549 AffiniPure Goat Anti-Mouse IgM, µ chain specific |
Jackson |
115-505-020 |
2.0mg |
| DyLight 549 AffiniPure Goat Anti-Mouse IgM, µ chain specific (min X Hu, Bov, Hrs Sr Prot) |
Jackson |
115-505-075 |
1.0mg |
| DyLight 549 AffiniPure Rabbit Anti-Mouse IgG (H+L) |
Jackson |
315-505-003 |
1.5mg |
| DyLight 549 AffiniPure Rabbit Anti-Mouse IgG (H+L) (min X Hu Sr Prot) |
Jackson |
315-505-045 |
1.0mg |
| DyLight 549 AffiniPure Rabbit Anti-Mouse IgG, Fcγ fragment specific |
Jackson |
315-505-008 |
1.5mg |
| DyLight 549 AffiniPure Rabbit Anti-Mouse IgG, Fcγ fragment specific (min X Hu Sr Prot) |
Jackson |
315-505-046 |
1.0mg |
| DyLight 549 AffiniPure Rabbit Anti-Mouse IgG, F(ab')2 fragment specific |
Jackson |
315-505-006 |
1.5mg |
| DyLight 549 AffiniPure Rabbit Anti-Mouse IgG, F(ab')2 fragment specific (min X Hu Sr Prot) |
Jackson |
315-505-047 |
1.0mg |
| DyLight 549 AffiniPure Rabbit Anti-Mouse IgG + IgM (H+L) |
Jackson |
315-505-044 |
1.5mg |
| DyLight 549 AffiniPure Rabbit Anti-Mouse IgG + IgM (H+L) (min X Hu Sr Prot) |
Jackson |
315-505-048 |
1.0mg |
| DyLight 549 AffiniPure Rabbit Anti-Mouse IgM, µ chain specific |
Jackson |
315-505-020 |
1.5mg |
| DyLight 549 AffiniPure Rabbit Anti-Mouse IgM, µ chain specific (min X Hu Sr Prot) |
Jackson |
315-505-049 |
1.0mg |
| DyLight 549 AffiniPure Rat Anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs, Rat, Rb Sr Prot) |
Jackson |
415-505-166 |
1.0mg |
| DyLight 549 AffiniPure Sheep Anti-Mouse IgG (H+L) |
Jackson |
515-505-003 |
2.0mg |
| DyLight 549 AffiniPure Sheep Anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) |
Jackson |
515-505-062 |
1.5mg |
| DyLight 549 AffiniPure Sheep Anti-Mouse IgG, Fcγ fragment specific (min X Hu, Bov, Hrs Sr Prot) |
Jackson |
515-505-071 |
1.5mg |
| DyLight 549 AffiniPure Sheep Anti-Mouse IgG, F(ab')2 fragment specific (min X Hu, Bov, Hrs Sr Prot) |
Jackson |
515-505-072 |
1.5mg |
2、Jackson DyLight 549抗兔IgG二抗产品如下：
| 品名 |
品牌 |
货号 |
规格 |
| DyLight 549 AffiniPure Donkey Anti-Rabbit IgG (H+L) (min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Ms, Rat, Shp Sr Prot) |
Jackson |
711-505-152 |
0.5mg |
| DyLight 549 AffiniPure Goat Anti-Rabbit IgG (H+L) |
Jackson |
111-505-003 |
2.0mg |
| DyLight 549 AffiniPure Goat Anti-Rabbit IgG (H+L) (min X Hu Sr Prot) |
Jackson |
111-505-045 |
1.5mg |
| DyLight 549 AffiniPure Goat Anti-Rabbit IgG (H+L) (min X Hu, Ms, Rat Sr Prot) |
Jackson |
111-505-144 |
1.5mg |
| DyLight 549 AffiniPure IgG Fraction Monoclonal Mouse Anti-Rabbit IgG, Light Chain Specific for Western blotting (min X Bov, Gt, Ar Hms, Hrs, Hu, Ms, Rat, Shp Ig) |
Jackson |
211-502-171 |
0.5mg |
| DyLight 549 AffiniPure Goat Anti-Rabbit IgG, Fc fragment specific |
Jackson |
111-505-008 |
2.0mg |
| DyLight 549 AffiniPure Goat Anti-Rabbit IgG, Fc fragment specific (min X Hu Sr Prot) |
Jackson |
111-505-046 |
1.5mg |
| DyLight 549 AffiniPure Goat Anti-Rabbit IgG, F(ab')2 fragment specific |
Jackson |
111-505-006 |
2.0mg |
| DyLight 549 AffiniPure Goat Anti-Rabbit IgG, F(ab')2 fragment specific (min X Hu Sr Prot) |
Jackson |
111-505-047 |
1.5mg |
| DyLight 549 AffiniPure Polyclonal Mouse Anti-Rabbit IgG (H+L) (min X Hu, Gt, Ms, Shp Sr Prot) |
Jackson |
211-505-109 |
1.0mg |
3、Jackson DyLight 549抗山羊二抗产品如下：
| 品名 |
品牌 |
货号 |
规格 |
| DyLight 549 AffiniPure Bovine Anti-Goat IgG (H+L) (min X Bov, Ck, GP, Sy Hms, Hrs, Hu, Ms, Rb, Rat Sr Prot) |
Jackson |
805-505-180 |
0.5mg |
| DyLight 549 AffiniPure Donkey Anti-Goat IgG (H+L) |
Jackson |
705-505-003 |
1.0mg |
| DyLight 549 AffiniPure Donkey Anti-Goat IgG (H+L) (min X Ck, GP, Sy Hms, Hrs, Hu, Ms, Rb, Rat Sr Prot) |
Jackson |
705-505-147 |
0.5mg |
| DyLight 549 AffiniPure Mouse Anti-Goat IgG (H+L) (min X Ms, Hu, Rb Sr Prot) |
Jackson |
205-505-108 |
1.0mg |
| DyLight 549 AffiniPure Rabbit Anti-Goat IgG (H+L) |
Jackson |
305-505-003 |
1.5mg |
| DyLight 549 AffiniPure Rabbit Anti-Goat IgG (H+L) (min X Hu Sr Prot) |
Jackson |
305-505-045 |
1.0mg |
| DyLight 549 AffiniPure Rabbit Anti-Goat IgG, Fc fragment specific |
Jackson |
305-505-008 |
1.5mg |
| DyLight 549 AffiniPure Rabbit Anti-Goat IgG, Fc fragment specific (min X Hu Sr Prot) |
Jackson |
305-505-046 |
1.0mg |
| DyLight 549 AffiniPure Rabbit Anti-Goat IgG, F(ab')2 fragment specific |
Jackson |
305-505-006 |
1.5mg |
| DyLight 549 AffiniPure Rabbit Anti-Goat IgG, F(ab')2 fragment specific (min X Hu Sr Prot) |
Jackson |
305-505-047 |
1.0mg |
4、Jackson DyLight 549抗大鼠二抗产品如下：
| 品名 |
品牌 |
货号 |
规格 |
| DyLight 549 AffiniPure Donkey Anti-Rat IgG (H+L) (min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Rb, Shp Sr Prot) |
Jackson |
712-505-150 |
0.5mg |
| DyLight 549 AffiniPure Donkey Anti-Rat IgG (H+L) (min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Ms, Rb, Shp Sr Prot) |
Jackson |
712-505-153 |
0.5mg |
| DyLight 549 AffiniPure Goat Anti-Rat IgG (H+L) |
Jackson |
112-505-003 |
2.0mg |
| DyLight 549 AffiniPure Goat Anti-Rat IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) |
Jackson |
112-505-062 |
1.5mg |
| DyLight 549 AffiniPure Goat Anti-Rat IgG (H+L) (min X Hu, Bov, Hrs, Rb Sr Prot) |
Jackson |
112-505-143 |
1.5mg |
| DyLight 549 AffiniPure Goat Anti-Rat IgG (H+L) (min X Ms, Hu, Bov, Hrs, Rb Sr Prot) |
Jackson |
112-505-167 |
0.5mg |
| DyLight 549 AffiniPure Goat Anti-Rat IgG, Light Chain Specific for Western blotting (min X Bov, Gt, Hrs, Hu, Ms, Rat, Shp Ig) |
Jackson |
112-505-175 |
0.5mg |
| DyLight 549 AffiniPure Goat Anti-Rat IgG, Fcγ fragment specific |
Jackson |
112-505-008 |
2.0mg |
| DyLight 549 AffiniPure Goat Anti-Rat IgG, Fcγ fragment specific (min X Hu, Bov, Hrs Sr Prot) |
Jackson |
112-505-071 |
1.5mg |
| DyLight 549 AffiniPure Goat Anti-Rat IgG, F(ab')2 fragment specific |
Jackson |
112-505-006 |
2.0mg |
| DyLight 549 AffiniPure Goat Anti-Rat IgG, F(ab')2 fragment specific (min X Hu, Bov, Hrs Sr Prot) |
Jackson |
112-505-072 |
1.5mg |
| DyLight 549 AffiniPure Goat Anti-Rat IgG + IgM (H+L) |
Jackson |
112-505-044 |
2.0mg |
| DyLight 549 AffiniPure Goat Anti-Rat IgG + IgM (H+L) (min X Hu, Bov, Hrs Sr Prot) |
Jackson |
112-505-068 |
1.5mg |
| DyLight 549 AffiniPure Goat Anti-Rat IgM, µ chain specific |
Jackson |
112-505-020 |
2.0mg |
| DyLight 549 AffiniPure Goat Anti-Rat IgM, µ chain specific (min X Hu, Bov, Hrs Sr Prot) |
Jackson |
112-505-075 |
1.0mg |
| DyLight 549 AffiniPure Mouse Anti-Rat IgG (H+L) (min X Ms Sr Prot) |
Jackson |
212-505-082 |
1.5mg |
| DyLight 549 AffiniPure Mouse Anti-Rat IgG (H+L) (min X Hu, Bov, Hrs, Ms, Gt, Rb Sr Prot) |
Jackson |
212-505-168 |
1.0mg |
| DyLight 549 AffiniPure Mouse Anti-Rat IgG, Fcγ fragment specific (min X Hu, Bov, Hrs, Ms Sr Prot) |
Jackson |
212-505-104 |
1.0mg |
| DyLight 549 AffiniPure Mouse Anti-Rat IgG, F(ab')2 fragment specific (min X Hu, Bov, Hrs, Ms Sr Prot) |
Jackson |
212-505-106 |
1.0mg |
| DyLight 549 AffiniPure Rabbit Anti-Rat IgG (H+L) |
Jackson |
312-505-003 |
1.5mg |
| DyLight 549 AffiniPure Rabbit Anti-Rat IgG (H+L) (min X Hu Sr Prot) |
Jackson |
312-505-045 |
1.0mg |
| DyLight 549 AffiniPure Rabbit Anti-Rat IgG, Fcγ fragment specific (min X Hu Sr Prot) |
Jackson |
312-505-046 |
1.0mg |
| DyLight 549 AffiniPure Rabbit Anti-Rat IgG, F(ab')2 fragment specific (min X Hu Sr Prot) |
Jackson |
312-505-047 |
1.0mg |
| DyLight 549 AffiniPure Rabbit Anti-Rat IgG + IgM (H+L) |
Jackson |
312-505-044 |
1.5mg |
| DyLight 549 AffiniPure Rabbit Anti-Rat IgG + IgM (H+L) (min X Hu Sr Prot) |
Jackson |
312-505-048 |
1.0mg |
| DyLight 549 AffiniPure Rabbit Anti-Rat IgM, µ chain specific |
Jackson |
312-505-020 |
1.5mg |
| DyLight 549 AffiniPure Rabbit Anti-Rat IgM, µ chain specific (min X Hu Sr Prot) |
Jackson |
312-505-049 |
1.0mg |
5、Jackson DyLight 549抗人二抗产品如下：
| 品名 |
品牌 |
货号 |
规格 |
| DyLight 549 AffiniPure Donkey Anti-Human IgG (H+L) (min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Ms, Rb, Rat, Shp Sr Prot) |
Jackson |
709-505-149 |
0.5mg |
| DyLight 549 AffiniPure Donkey Anti-Human IgG, Fcγ fragment specific (min X Bov, Hrs, Ms Sr Prot) |
Jackson |
709-505-098 |
1.0mg |
| DyLight 549 AffiniPure Donkey Anti-Human IgM, Fc5µ fragment specific (min X Bov, Hrs Sr Prot) |
Jackson |
709-505-073 |
1.5mg |
| DyLight 549 AffiniPure Goat Anti-Human IgG (H+L) |
Jackson |
109-505-003 |
2.0mg |
| DyLight 549 AffiniPure Goat Anti-Human IgG (H+L) (min X Bov, Hrs, Ms Sr Prot) |
Jackson |
109-505-088 |
1.5mg |
| DyLight 549 AffiniPure Goat Anti-Human IgG, Fcγ fragment specific |
Jackson |
109-505-008 |
2.0mg |
| DyLight 549 AffiniPure Goat Anti-Human IgG, Fcγ fragment specific (min X Bov, Hrs, Ms Sr Prot) |
Jackson |
109-505-098 |
1.0mg |
| DyLight 549 AffiniPure Goat Anti-Human IgG, F(ab')2 fragment specific |
Jackson |
109-505-006 |
2.0mg |
| DyLight 549 AffiniPure Goat Anti-Human IgG, F(ab')2 fragment specific (min X Bov, Hrs, Ms Sr Prot) |
Jackson |
109-505-097 |
1.5mg |
| DyLight 549 AffiniPure Goat Anti-Human IgG + IgM (H+L) |
Jackson |
109-505-044 |
2.0mg |
| DyLight 549 AffiniPure Goat Anti-Human IgG + IgM (H+L) (min X Bov Sr Prot) |
Jackson |
109-505-127 |
1.5mg |
| DyLight 549 AffiniPure Goat Anti-Human IgA + IgG + IgM (H+L) |
Jackson |
109-505-064 |
2.0mg |
| DyLight 549 AffiniPure Goat Anti-Human IgM, Fc5µ fragment specific |
Jackson |
109-505-043 |
2.0mg |
| DyLight 549 AffiniPure Goat Anti-Human IgM, Fc5µ fragment specific (min X Bov Sr Prot) |
Jackson |
109-505-129 |
1.5mg |
| DyLight 549 AffiniPure Goat Anti-Human Serum IgA, α chain specific |
Jackson |
109-505-011 |
2.0mg |
| DyLight 549 AffiniPure Mouse Anti-Human IgG (H+L) (min X Ms Sr Prot) |
Jackson |
209-505-082 |
1.5mg |
| DyLight 549 AffiniPure Mouse Anti-Human IgG (H+L) (min X Bov, Hrs, Ms Sr Prot) |
Jackson |
209-505-088 |
1.0mg |
| DyLight 549 AffiniPure Mouse Anti-Human IgG, Fcγ fragment specific (min X Bov, Hrs, Ms Sr Prot) |
Jackson |
209-505-098 |
1.0mg |
| DyLight 549 AffiniPure Mouse Anti-Human IgG, F(ab')2 fragment specific (min X Bov, Hrs, Ms Sr Prot) |
Jackson |
209-505-097 |
1.0mg |
| DyLight 549 AffiniPure Rabbit Anti-Human IgG (H+L) |
Jackson |
309-505-003 |
1.5mg |
| DyLight 549 AffiniPure Rabbit Anti-Human IgG (H+L) (min X Ms Sr Prot) |
Jackson |
309-505-082 |
1.0mg |
| DyLight 549 AffiniPure Rabbit Anti-Human IgG, Fcγ fragment specific |
Jackson |
309-505-008 |
1.5mg |
| DyLight 549 AffiniPure Rabbit Anti-Human IgG, F(ab')2 fragment specific |
Jackson |
309-505-006 |
1.5mg |
| DyLight 549 AffiniPure Rabbit Anti-Human IgG + IgM (H+L) (min X Ms Sr Prot) |
Jackson |
309-505-107 |
1.0mg |
| DyLight 549 AffiniPure Rabbit Anti-Human IgA + IgG + IgM (H+L) |
Jackson |
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