DyLigh™系列荧光标记是一种近年来被广泛应用的新型荧光染料,由于具有很好的光谱宽度、更强的荧光强度,更高的光学耐受性(抗淬灭性)、对pH不敏感、分子较小而渗透性更好的优势,越来越受到科研客户的喜爱。DyLight™种类齐全,如Dylight 405是蓝色荧光,替代Alexa 405;DyLight 594是绿色荧光,可完全替代FITC/Cy2/Alexa 488;完全替代Cy3/Rhodamin/Alexa 546/Alexa 555的Dylight 549;替代Texas Red/Alexa 594的Dylight 594;Dylight 633和Dylight 649(可替代Cy5/Alexa 647)则是更强的红色荧光;另外,Dylight还有适配Odyssey系统的近红外和远红外二抗Dylight 680和DyLight 800。
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图 1:HEp-2细胞使用ANA一抗(BIO-RAD),并比较使用不同发色团的二抗标记。荧光显微镜下观察ProLong Gold (Invitrogen)封片的细胞。Nikon LABOPHOTNikon 荧光显微镜连接D-70 DSLR 摄像机,100×曝光拍摄的图片。图中Alexa 594和DyLight 594标记的细胞1秒曝光所拍,Texas Red标记的细胞2秒曝光所拍。其它封片剂处理的细胞获得类似的结果。
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图2、以时间为横坐标,初始荧光强度的百分比为纵坐标绘制的荧光强度图表,HEp-2细胞以ANA(Bio-Rad)和各种红色荧光标记的山羊抗人IgG(H+L)的二抗标记。细胞分别用ProLong Gold(左)、Vectashield(中)、含N-propyl gallate的 PBS-90%甘油封片(右)。由上图所示,ProLong Gold 封片的细胞经DyLight 594标记显色,耐光性强于Texas Red,与Alexa 594相当。Vectashield封片的细胞用DyLight 594和Alexa 594标记,耐光性弱于Texas Red。然而含N-propyl gallate的PBS-90%甘油封片的细胞DyLight 594标记抗体,耐光性强于其他标记抗体。
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DyLight 594标记的抗体荧光亮度明显强于Alexa 594标记抗体。相比较Texas Red而言,不仅更亮,而且具有更好的水溶性。与DyLight 549、Cy3或TRITC标记物相比,它与绿色标记染料表现出更大的色差;就其光学稳定性,DyLight 594在不含有抗淬灭剂的条件下,远较Texas Red稳定,与Alexa594相当;在含有某些抗淬灭剂的情况下,DyLight 594同样表现优异。总之,在可见光谱的深红色区域内,DyLight 594标记二抗是所有免疫荧光实验中最好的选择。另外,DyLight荧光标记的二抗比传统的FITC/Cy2以及Alexa 488具有更高的性价比。
我们向您推荐美国Abbkine原装进口的液体形式的小包装常用DyLight系列二抗产品,以及Jackson各种规格的全系列DyLight二抗产品。Abbkine是美国专业生产免疫试剂并提供细胞学研究解决方案的公司,其产品主要销往到全球的的大学、科研院所等研究机构。其中Abbkine为您提供 最常用的DyLight 594荧光标记二抗产品:
品名 |
品牌 |
货号 |
规格 |
DyLight 594 AffiniPure Goat Anti-Mouse IgG(H+L) |
Abbkine |
A23410 |
100μl/500μl |
DyLight 594 AffiniPure Goat Anti-Rabbit IgG(H+L) |
Abbkine |
A23420 |
100μl/500μl |
DyLight 594 AffiniPure Rabbit Anti-Goat IgG(H+L) |
Abbkine |
A23430 |
100μl/500μl |
DyLight 594 AffiniPure Goat Anti-Rat IgG(H+L) |
Abbkine |
A23440 |
100μl/500μl |
Jackson 是全球专业从事生产和销售亲和纯化的二抗和纯化免疫球蛋白的公司,其抗体产品线完全,有全球最大的二抗产品库,能提供多种应用类型和需要的二抗产品,其二抗产品本身也具有极高的产品质量和稳定性,其产品主要销往到世界各地的大学科研所和医院里的动物研究以及生物医学研究机构的科学家。Jackson能为您提供最全的DyLight 594荧光标记二抗:
1、Jackson DyLight 594抗小鼠IgG二抗产品如下:
品名 |
品牌 |
货号 |
规格 |
DyLight 594 AffiniPure Donkey Anti-Mouse IgG (H+L) (min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Rb, Shp Sr Prot) |
Jackson |
715-515-150 |
0.5mg |
DyLight 594 AffiniPure Donkey Anti-Mouse IgG (H+L) (min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Rb, Rat, Shp Sr Prot) |
Jackson |
715-515-151 |
0.5mg |
DyLight 594 AffiniPure Donkey Anti-Mouse IgM, µ chain specific |
Jackson |
715-515-020 |
2.0mg |
DyLight 594 AffiniPure Donkey Anti-Mouse IgM, µ chain specific (min X Hu, Bov, Hrs, Rat Sr Prot) |
Jackson |
715-515-140 |
0.5mg |
DyLight 594 AffiniPure Goat Anti-Mouse IgG (H+L) |
Jackson |
115-515-003 |
2.0mg |
DyLight 594 AffiniPure Goat Anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) |
Jackson |
115-515-062 |
1.5mg |
DyLight 594 AffiniPure Goat Anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs, Rb, Sw Sr Prot) |
Jackson |
115-515-146 |
1.5mg |
DyLight 594 AffiniPure Goat Anti-Mouse IgG (H+L) (min X Rat, Hu, Bov, Hrs, Rb, Sr Prot) |
Jackson |
115-515-166 |
0.5mg |
DyLight 594 AffiniPure Goat Anti-Mouse IgG, Light Chain Specific for Western blotting (min X Bov, Gt, Hrs, Hu, Rb, Rat, Shp Ig) |
Jackson |
115-515-174 |
0.5mg |
DyLight 594 AffiniPure Goat Anti-Mouse IgG, Fcγ fragment specific |
Jackson |
115-515-008 |
2.0mg |
DyLight 594 AffiniPure Goat Anti-Mouse IgG, Fcγ fragment specific (min X Hu, Bov, Hrs Sr Prot) |
Jackson |
115-515-071 |
1.5mg |
DyLight 594 AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 1 specific (min X Hu, Bov, Rb Sr Prot) |
Jackson |
115-515-205 |
0.5mg |
DyLight 594 AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2a specific (min X Hu, Bov, Rb Sr Prot) |
Jackson |
115-515-206 |
0.5mg |
DyLight 594 AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2b specific (min X Hu, Bov, Rb Sr Prot) |
Jackson |
115-515-207 |
0.5mg |
DyLight 594 AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2c specific (min X Hu, Bov, Rb Sr Prot) |
Jackson |
115-515-208 |
0.5mg |
DyLight 594 AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 3 specific (min X Hu, Bov, Rb Sr Prot) |
Jackson |
115-515-209 |
0.5mg |
DyLight 594 AffiniPure Goat Anti-Mouse IgG (subclasses 1+2a+2b+3), Fcγ fragment specific (min X Hu,Bov, Rb Sr Prot) |
Jackson |
115-515-164 |
1.0mg |
DyLight 594 AffiniPure Goat Anti-Mouse IgG, F(ab')2 fragment specific |
Jackson |
115-515-006 |
2.0mg |
DyLight 594 AffiniPure Goat Anti-Mouse IgG, F(ab')2 fragment specific (min X Hu, Bov, Hrs Sr Prot) |
Jackson |
115-515-072 |
1.5mg |
DyLight 594 AffiniPure Goat Anti-Mouse IgG + IgM (H+L) |
Jackson |
115-515-044 |
2.0mg |
DyLight 594 AffiniPure Goat Anti-Mouse IgG + IgM(H+L) (min X Hu, Bov,Hrs Sr Prot) |
Jackson |
115-515-068 |
1.5mg |
DyLight 594 AffiniPure Goat Anti-Mouse IgM, µ chain specific |
Jackson |
115-515-020 |
2.0mg |
DyLight 594 AffiniPure Goat Anti-Mouse IgM, µ chain specific (min X Hu, Bov, Hrs Sr Prot) |
Jackson |
115-515-075 |
1.0mg |
DyLight 594 AffiniPure Rabbit Anti-Mouse IgG (H+L) |
Jackson |
315-515-003 |
1.5mg |
DyLight 594 AffiniPure Rabbit Anti-Mouse IgG (H+L) (min X Hu Sr Prot) |
Jackson |
315-515-045 |
1.0mg |
DyLight 594 AffiniPure Rabbit Anti-Mouse IgG, Fcγ fragment specific |
Jackson |
315-515-008 |
1.5mg |
DyLight 594 AffiniPure Rabbit Anti-Mouse IgG, Fcγ fragment specific (min X Hu Sr Prot) |
Jackson |
315-515-046 |
1.0mg |
DyLight 594 AffiniPure Rabbit Anti-Mouse IgG, F(ab')2 fragment specific |
Jackson |
315-515-006 |
1.5mg |
DyLight 594 AffiniPure Rabbit Anti-Mouse IgG, F(ab')2 fragment specific (min X Hu Sr Prot) |
Jackson |
315-515-047 |
1.0mg |
DyLight 594 AffiniPure Rabbit Anti-Mouse IgG + IgM (H+L) |
Jackson |
315-515-044 |
1.5mg |
DyLight 594 AffiniPure Rabbit Anti-Mouse IgG + IgM (H+L) (min X Hu Sr Prot) |
Jackson |
315-515-048 |
1.0mg |
DyLight 594 AffiniPure Rabbit Anti-Mouse IgM, µ chain specific |
Jackson |
315-515-020 |
1.5mg |
DyLight 594 AffiniPure Rabbit Anti-Mouse IgM, µ chain specific (min X Hu Sr Prot) |
Jackson |
315-515-049 |
1.0mg |
DyLight 594 AffiniPure Rat Anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs, Rat, Rb Sr Prot) |
Jackson |
415-515-166 |
1.0mg |
DyLight 594 AffiniPure Sheep Anti-Mouse IgG (H+L) |
Jackson |
515-515-003 |
2.0mg |
DyLight 594 AffiniPure Sheep Anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) |
Jackson |
515-515-062 |
1.5mg |
DyLight 594 AffiniPure Sheep Anti-Mouse IgG, Fcγ fragment specific (min X Hu, Bov, Hrs Sr Prot) |
Jackson |
515-515-071 |
1.5mg |
DyLight 594 AffiniPure Sheep Anti-Mouse IgG, F(ab')2 fragment specific (min X Hu, Bov, Hrs Sr Prot) |
Jackson |
515-515-072 |
1.5mg |
2、Jackson DyLight 594抗兔IgG二抗产品如下:
品名 |
品牌 |
货号 |
规格 |
DyLight 594 AffiniPure Donkey Anti-Rabbit IgG (H+L) (min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Ms, Rat, Shp Sr Prot) |
Jackson |
711-515-152 |
0.5mg |
DyLight 594 AffiniPure Goat Anti-Rabbit IgG (H+L) |
Jackson |
111-515-003 |
2.0mg |
DyLight 594 AffiniPure Goat Anti-Rabbit IgG (H+L) (min X Hu Sr Prot) |
Jackson |
111-515-045 |
1.5mg |
DyLight 594 AffiniPure Goat Anti-Rabbit IgG (H+L) (min X Hu, Ms, Rat Sr Prot) |
Jackson |
111-515-144 |
1.5mg |
DyLight 594 AffiniPure IgG Fraction Monoclonal Mouse Anti-Rabbit IgG, Light Chain Specific for Western blotting (min X Bov, Gt, Ar Hms, Hrs, Hu, Ms, Rat, Shp Ig) |
Jackson |
211-512-171 |
0.5mg |
DyLight 594 AffiniPure Goat Anti-Rabbit IgG, Fc fragment specific |
Jackson |
111-515-008 |
2.0mg |
DyLight 594 AffiniPure Goat Anti-Rabbit IgG, Fc fragment specific (min X Hu Sr Prot) |
Jackson |
111-515-046 |
1.5mg |
DyLight 594 AffiniPure Goat Anti-Rabbit IgG, F(ab')2 fragment specific |
Jackson |
111-515-006 |
2.0mg |
DyLight 594 AffiniPure Goat Anti-Rabbit IgG, F(ab')2 fragment specific (min X Hu Sr Prot) |
Jackson |
111-515-047 |
1.5mg |
DyLight 594 AffiniPure Polyclonal Mouse Anti-Rabbit IgG (H+L) (min X Hu, Gt, Ms, Shp Sr Prot) |
Jackson |
211-515-109 |
1.0mg |
3、Jackson DyLight 594抗山羊二抗产品如下:
品名 |
品牌 |
货号 |
规格 |
DyLight 594 AffiniPure Bovine Anti-Goat IgG (H+L) (min X Bov, Ck, GP, Sy Hms, Hrs, Hu, Ms, Rb, Rat Sr Prot) |
Jackson |
805-515-180 |
0.5mg |
DyLight 594 AffiniPure Donkey Anti-Goat IgG (H+L) |
Jackson |
705-515-003 |
1.0mg |
DyLight 594 AffiniPure Donkey Anti-Goat IgG (H+L) (min X Ck, GP, Sy Hms, Hrs, Hu, Ms, Rb, Rat Sr Prot) |
Jackson |
705-515-147 |
0.5mg |
DyLight 594 AffiniPure Mouse Anti-Goat IgG (H+L) (min X Ms, Hu, Rb Sr Prot) |
Jackson |
205-515-108 |
1.0mg |
DyLight 594 AffiniPure Rabbit Anti-Goat IgG (H+L) |
Jackson |
305-515-003 |
1.5mg |
DyLight 594 AffiniPure Rabbit Anti-Goat IgG (H+L) (min X Hu Sr Prot) |
Jackson |
305-515-045 |
1.0mg |
DyLight 594 AffiniPure Rabbit Anti-Goat IgG, Fc fragment specific |
Jackson |
305-515-008 |
1.5mg |
DyLight 594 AffiniPure Rabbit Anti-Goat IgG, Fc fragment specific (min X Hu Sr Prot) |
Jackson |
305-515-046 |
1.0mg |
DyLight 594 AffiniPure Rabbit Anti-Goat IgG, F(ab')2 fragment specific |
Jackson |
305-515-006 |
1.5mg |
DyLight 594 AffiniPure Rabbit Anti-Goat IgG, F(ab')2 fragment specific (min X Hu Sr Prot) |
Jackson |
305-515-047 |
1.0mg |
4、Jackson DyLight 594抗大鼠二抗产品如下:
品名 |
品牌 |
货号 |
规格 |
DyLight 594 AffiniPure Donkey Anti-Rat IgG (H+L) (min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Rb, Shp Sr Prot) |
Jackson |
712-515-150 |
0.5mg |
DyLight 594 AffiniPure Donkey Anti-Rat IgG (H+L) (min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Ms, Rb, Shp Sr Prot) |
Jackson |
712-515-153 |
0.5mg |
DyLight 594 AffiniPure Goat Anti-Rat IgG (H+L) |
Jackson |
112-515-003 |
2.0mg |
DyLight 594 AffiniPure Goat Anti-Rat IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) |
Jackson |
112-515-062 |
1.5mg |
DyLight 594 AffiniPure Goat Anti-Rat IgG (H+L) (min X Hu, Bov, Hrs, Rb Sr Prot) |
Jackson |
112-515-143 |
1.5mg |
DyLight 594 AffiniPure Goat Anti-Rat IgG (H+L) (min X Ms, Hu, Bov, Hrs, Rb Sr Prot) |
Jackson |
112-515-167 |
0.5mg |
DyLight 594 AffiniPure Goat Anti-Rat IgG, Light Chain Specific for Western blotting (min X Bov, Gt, Hrs, Hu, Ms, Rat, Shp Ig) |
Jackson |
112-515-175 |
0.5mg |
DyLight 594 AffiniPure Goat Anti-Rat IgG, Fcγ fragment specific |
Jackson |
112-515-008 |
2.0mg |
DyLight 594 AffiniPure Goat Anti-Rat IgG, Fcγ fragment specific (min X Hu, Bov, Hrs Sr Prot) |
Jackson |
112-515-071 |
1.5mg |
DyLight 594 AffiniPure Goat Anti-Rat IgG, F(ab')2 fragment specific |
Jackson |
112-515-006 |
2.0mg |
DyLight 594 AffiniPure Goat Anti-Rat IgG, F(ab')2 fragment specific (min X Hu, Bov, Hrs Sr Prot) |
Jackson |
112-515-072 |
1.5mg |
DyLight 594 AffiniPure Goat Anti-Rat IgG + IgM (H+L) |
Jackson |
112-515-044 |
2.0mg |
DyLight 594 AffiniPure Goat Anti-Rat IgG + IgM (H+L) (min X Hu, Bov, Hrs Sr Prot) |
Jackson |
112-515-068 |
1.5mg |
DyLight 594 AffiniPure Goat Anti-Rat IgM, µ chain specific |
Jackson |
112-515-020 |
2.0mg |
DyLight 594 AffiniPure Goat Anti-Rat IgM, µ chain specific (min X Hu, Bov, Hrs Sr Prot) |
Jackson |
112-515-075 |
1.0mg |
DyLight 594 AffiniPure Mouse Anti-Rat IgG (H+L) (min X Ms Sr Prot) |
Jackson |
212-515-082 |
1.5mg |
DyLight 594 AffiniPure Mouse Anti-Rat IgG (H+L) (min X Hu, Bov, Hrs, Ms, Gt, Rb Sr Prot) |
Jackson |
212-515-168 |
1.0mg |
DyLight 594 AffiniPure Mouse Anti-Rat IgG, Fcγ fragment specific (min X Hu, Bov, Hrs, Ms Sr Prot) |
Jackson |
212-515-104 |
1.0mg |
DyLight 594 AffiniPure Mouse Anti-Rat IgG, F(ab')2 fragment specific (min X Hu, Bov, Hrs, Ms Sr Prot) |
Jackson |
212-515-106 |
1.0mg |
DyLight 594 AffiniPure Rabbit Anti-Rat IgG (H+L) |
Jackson |
312-515-003 |
1.5mg |
DyLight 594 AffiniPure Rabbit Anti-Rat IgG (H+L) (min X Hu Sr Prot) |
Jackson |
312-515-045 |
1.0mg |
DyLight 594 AffiniPure Rabbit Anti-Rat IgG, Fcγ fragment specific (min X Hu Sr Prot) |
Jackson |
312-515-046 |
1.0mg |
DyLight 594 AffiniPure Rabbit Anti-Rat IgG, F(ab')2 fragment specific (min X Hu Sr Prot) |
Jackson |
312-515-047 |
1.0mg |
DyLight 594 AffiniPure Rabbit Anti-Rat IgG + IgM (H+L) |
Jackson |
312-515-044 |
1.5mg |
DyLight 594 AffiniPure Rabbit Anti-Rat IgG + IgM (H+L) (min X Hu Sr Prot) |
Jackson |
312-515-048 |
1.0mg |
DyLight 594 AffiniPure Rabbit Anti-Rat IgM, µ chain specific |
Jackson |
312-515-020 |
1.5mg |
DyLight 594 AffiniPure Rabbit Anti-Rat IgM, µ chain specific (min X Hu Sr Prot) |
Jackson |
312-515-049 |
1.0mg |
5、Jackson DyLight 594抗人二抗产品如下:
品名 |
品牌 |
货号 |
规格 |
DyLight 594 AffiniPure Donkey Anti-Human IgG (H+L) (min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Ms, Rb, Rat, Shp Sr Prot) |
Jackson |
709-515-149 |
0.5mg |
DyLight 594 AffiniPure Donkey Anti-Human IgG, Fcγ fragment specific (min X Bov, Hrs, Ms Sr Prot) |
Jackson |
709-515-098 |
1.0mg |
DyLight 594 AffiniPure Donkey Anti-Human IgM, Fc5µ fragment specific (min X Bov, Hrs Sr Prot) |
Jackson |
709-515-073 |
1.5mg |
DyLight 594 AffiniPure Goat Anti-Human IgG (H+L) |
Jackson |
109-515-003 |
2.0mg |
DyLight 594 AffiniPure Goat Anti-Human IgG (H+L) (min X Bov, Hrs, Ms Sr Prot) |
Jackson |
109-515-088 |
1.5mg |
DyLight 594 AffiniPure Goat Anti-Human IgG, Fcγ fragment specific |
Jackson |
109-515-008 |
2.0mg |
DyLight 594 AffiniPure Goat Anti-Human IgG, Fcγ fragment specific (min X Bov, Hrs, Ms Sr Prot) |
Jackson |
109-515-098 |
1.0mg |
DyLight 594 AffiniPure Goat Anti-Human IgG, F(ab')2 fragment specific |
Jackson |
109-515-006 |
2.0mg |
DyLight 594 AffiniPure Goat Anti-Human IgG, F(ab')2 fragment specific (min X Bov, Hrs, Ms Sr Prot) |
Jackson |
109-515-097 |
1.5mg |
DyLight 594 AffiniPure Goat Anti-Human IgG + IgM (H+L) |
Jackson |
109-515-044 |
2.0mg |
DyLight 594 AffiniPure Goat Anti-Human IgG + IgM (H+L) (min X Bov Sr Prot) |
Jackson |
109-515-127 |
1.5mg |
DyLight 594 AffiniPure Goat Anti-Human IgA + IgG + IgM (H+L) |
Jackson |
109-515-064 |
2.0mg |
DyLight 594 AffiniPure Goat Anti-Human IgM, Fc5µ fragment specific |
Jackson |
109-515-043 |
2.0mg |
DyLight 594 AffiniPure Goat Anti-Human IgM, Fc5µ fragment specific (min X Bov Sr Prot) |
Jackson |
109-515-129 |
1.5mg |
DyLight 594 AffiniPure Goat Anti-Human Serum IgA, α chain specific |
Jackson |
109-515-011 |
2.0mg |
DyLight 594 AffiniPure Mouse Anti-Human IgG (H+L) (min X Ms Sr Prot) |
Jackson |
209-515-082 |
1.5mg |
DyLight 594 AffiniPure Mouse Anti-Human IgG (H+L) (min X Bov, Hrs, Ms Sr Prot) |
Jackson |
209-515-088 |
1.0mg |
DyLight 594 AffiniPure Mouse Anti-Human IgG, Fcγ fragment specific (min X Bov, Hrs, Ms Sr Prot) |
Jackson |
209-515-098 |
1.0mg |
DyLight 594 AffiniPure Mouse Anti-Human IgG, F(ab')2 fragment specific (min X Bov, Hrs, Ms Sr Prot) |
Jackson |
209-515-097 |
1.0mg |
DyLight 594 AffiniPure Rabbit Anti-Human IgG (H+L) |
Jackson |
309-515-003 |
1.5mg |
DyLight 594 AffiniPure Rabbit Anti-Human IgG (H+L) (min X Ms Sr Prot) |
Jackson |
309-515-082 |
1.0mg |
DyLight 594 AffiniPure Rabbit Anti-Human IgG, Fcγ fragment specific |
Jackson |
309-515-008 |
1.5mg |
DyLight 594 AffiniPure Rabbit Anti-Human IgG, F(ab')2 fragment specific |
Jackson |
309-515-006 |
1.5mg |
DyLight 594 AffiniPure Rabbit Anti-Human IgG + IgM (H+L) (min X Ms Sr Prot) |
Jackson |
309-515-107 |
1.0mg |
DyLight 594 AffiniPure Rabbit Anti-Human IgA + IgG + IgM (H+L) |
Jackson |
309-515-064 |
1.5mg |
DyLight 594 AffiniPure Rabbit Anti-Human IgM, Fc5µ fragment specific (min X Ms Sr Prot) |
Jackson |
309-515-095 |
1.0mg |
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