EpiMark热启动Taq DNA聚合酶 货号：A-M0490L
EpiMark® Hot Start Taq DNA Polymerase Guidelines for PCR
背景资料：The Polymerase Chain Reaction (PCR) is a powerful and sensitive technique for DNA amplification. Taq DNA Polymerase is an enzyme widely used in PCR. EpiMark® Hot Start Taq DNA Polymerase allows for greater PCR sensitivity and permits room temperature PCR reaction set-up. The following guidelines are provided to ensure successful PCR using New England Biolabs’ EpiMark Hot Start Taq DNA Polymerase. These guidelines cover routine PCR.
产品描述：Quantify as little 20 femtograms of human DNA in as little as 1 µl of sample;High specificity and sensitivity for fungal DNA in a background of non-target DNA;Fast and simple: add samples to the PreMix... and quantify.
产品特点：
1Endonuclease Activity (Nicking):The product is tested in a reaction containing a supercoiled DNA substrate. After incubation for 4 hours the percent converted to the nicked form is determined by agarose gel electrophoresis；
2Inhibition of Primer Extension (Hot Start, Radioactivity Incorporation): The hot start polymerase is compared to the polymerase in non-hot start conditions using primer extension; inhibition is assessed by comparing radioactive incorporation；
3.PCR Amplification (Hot Start): The polymerase is tested in a hot start polymerase chain reaction (PCR) using Lambda DNA as the control template, specific primers and human genomic DNA, resulting in an increase in yield of the expected Lambda product and a decrease in non-specific genomic bands when compared to a non-hot start control reaction。
定购信息：
保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分。
